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EMBO J
2000 Oct 16;1920:5492-501. doi: 10.1093/emboj/19.20.5492.
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Human DNA ligase I efficiently seals nicks in nucleosomes.
Chafin DR
,
Vitolo JM
,
Henricksen LA
,
Bambara RA
,
Hayes JJ
.
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The access to DNA within nucleosomes is greatly restricted for most enzymes and trans-acting factors that bind DNA. We report here that human DNA ligase I, which carries out the final step of Okazaki fragment processing and of many DNA repair pathways, can access DNA that is wrapped about the surface of a nucleosome in vitro and carry out its enzymatic function with high efficiency. In addition, we find that ligase activity is not affected by the binding of linker histone (H1) but is greatly influenced by the disposition of the core histone tail domains. These results suggest that the window of opportunity for human DNA ligase I may extend well beyond the first stages of chromatin reassembly after DNA replication or repair.
Almouzni,
Chromatin assembly on replicating DNA in vitro.
1990, Pubmed,
Xenbase
Almouzni,
Chromatin assembly on replicating DNA in vitro.
1990,
Pubmed
,
Xenbase
Almouzni,
Assembly of spaced chromatin promoted by DNA synthesis in extracts from Xenopus eggs.
1988,
Pubmed
,
Xenbase
Anderson,
Sequence and position-dependence of the equilibrium accessibility of nucleosomal DNA target sites.
2000,
Pubmed
Anderson,
Metabolism of Okazaki fragments during simian virus 40 DNA replication.
1979,
Pubmed
Annunziato,
Maturation of nucleosomal and nonnucleosomal components of nascent chromatin: differential requirements for concurrent protein synthesis.
1982,
Pubmed
Bambara,
Enzymes and reactions at the eukaryotic DNA replication fork.
1997,
Pubmed
Bielinsky,
Chromosomal ARS1 has a single leading strand start site.
1999,
Pubmed
Böhm,
Proteases as structural probes for chromatin: the domain structure of histones.
1984,
Pubmed
Cirillo,
Binding of the winged-helix transcription factor HNF3 to a linker histone site on the nucleosome.
1998,
Pubmed
Cusick,
Structure of chromatin at deoxyribonucleic acid replication forks: nuclease hypersensitivity results from both prenucleosomal deoxyribonucleic acid and an immature chromatin structure.
1983,
Pubmed
Gaillard,
Initiation and bidirectional propagation of chromatin assembly from a target site for nucleotide excision repair.
1997,
Pubmed
,
Xenbase
Gale,
UV-induced formation of pyrimidine dimers in nucleosome core DNA is strongly modulated with a period of 10.3 bases.
1987,
Pubmed
Garcia-Ramirez,
Modulation of chromatin folding by histone acetylation.
1995,
Pubmed
Georgel,
Role of histone tails in nucleosome remodeling by Drosophila NURF.
1997,
Pubmed
Gordenin,
Repeat expansion--all in a flap?
1997,
Pubmed
Hayes,
In vitro reconstitution and analysis of mononucleosomes containing defined DNAs and proteins.
1997,
Pubmed
,
Xenbase
Hayes,
The interaction of transcription factors with nucleosomal DNA.
1992,
Pubmed
Herman,
Structure of chromatin at deoxyribonucleic acid replication forks: location of the first nucleosomes on newly synthesized simian virus 40 deoxyribonucleic acid.
1981,
Pubmed
Howe,
Transcriptionally active Xenopus laevis somatic 5 S ribosomal RNA genes are packaged with hyperacetylated histone H4, whereas transcriptionally silent oocyte genes are not.
1998,
Pubmed
,
Xenbase
Kingston,
ATP-dependent remodeling and acetylation as regulators of chromatin fluidity.
1999,
Pubmed
Lee,
A positive role for histone acetylation in transcription factor access to nucleosomal DNA.
1993,
Pubmed
,
Xenbase
Lee,
Linker DNA and H1-dependent reorganization of histone-DNA interactions within the nucleosome.
1998,
Pubmed
,
Xenbase
Liu,
Ultraviolet damage and nucleosome folding of the 5S ribosomal RNA gene.
2000,
Pubmed
,
Xenbase
Logie,
The core histone N-terminal domains are required for multiple rounds of catalytic chromatin remodeling by the SWI/SNF and RSC complexes.
1999,
Pubmed
Mann,
DNA damage can alter the stability of nucleosomes: effects are dependent on damage type.
1997,
Pubmed
,
Xenbase
Meersseman,
Mobile nucleosomes--a general behavior.
1992,
Pubmed
Pennings,
Linker histones H1 and H5 prevent the mobility of positioned nucleosomes.
1994,
Pubmed
Perlmann,
Specific glucocorticoid receptor binding to DNA reconstituted in a nucleosome.
1988,
Pubmed
Polach,
Effects of core histone tail domains on the equilibrium constants for dynamic DNA site accessibility in nucleosomes.
2000,
Pubmed
Polach,
Mechanism of protein access to specific DNA sequences in chromatin: a dynamic equilibrium model for gene regulation.
1995,
Pubmed
Prigent,
Aberrant DNA repair and DNA replication due to an inherited enzymatic defect in human DNA ligase I.
1994,
Pubmed
Reddy,
The complex pathology of trinucleotide repeats.
1997,
Pubmed
Seale,
Nucleosomes associated with newly replicated DNA have an altered conformation.
1978,
Pubmed
Sera,
Role of histone H1 as an architectural determinant of chromatin structure and as a specific repressor of transcription on Xenopus oocyte 5S rRNA genes.
1998,
Pubmed
,
Xenbase
Smerdon,
Site-specific DNA repair at the nucleosome level in a yeast minichromosome.
1990,
Pubmed
Smith,
Purification and characterization of CAF-I, a human cell factor required for chromatin assembly during DNA replication in vitro.
1989,
Pubmed
Smith,
Stepwise assembly of chromatin during DNA replication in vitro.
1991,
Pubmed
Sogo,
Structure of replicating simian virus 40 minichromosomes. The replication fork, core histone segregation and terminal structures.
1986,
Pubmed
Suquet,
UV damage to DNA strongly influences its rotational setting on the histone surface of reconstituted nucleosomes.
1993,
Pubmed
Tse,
Disruption of higher-order folding by core histone acetylation dramatically enhances transcription of nucleosomal arrays by RNA polymerase III.
1998,
Pubmed
,
Xenbase
Ura,
Histone acetylation: influence on transcription, nucleosome mobility and positioning, and linker histone-dependent transcriptional repression.
1997,
Pubmed
,
Xenbase
Usachenko,
Rearrangement of the histone H2A C-terminal domain in the nucleosome.
1994,
Pubmed
van Holde,
The nucleosome core particle: does it have structural and physiologic relevance?
1999,
Pubmed
Vettese-Dadey,
Role of the histone amino termini in facilitated binding of a transcription factor, GAL4-AH, to nucleosome cores.
1994,
Pubmed
Vignali,
ATP-dependent chromatin-remodeling complexes.
2000,
Pubmed
Vitolo,
The H3-H4 N-terminal tail domains are the primary mediators of transcription factor IIIA access to 5S DNA within a nucleosome.
2000,
Pubmed
,
Xenbase
Waga,
The DNA replication fork in eukaryotic cells.
1998,
Pubmed
Widom,
Physicochemical studies of the folding of the 100 A nucleosome filament into the 300 A filament. Cation dependence.
1986,
Pubmed
