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J Biol Chem
2010 Dec 17;28551:39790-800. doi: 10.1074/jbc.M110.151373.
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Gating of a G protein-sensitive mammalian Kir3.1 prokaryotic Kir channel chimera in planar lipid bilayers.
Leal-Pinto E
,
Gómez-Llorente Y
,
Sundaram S
,
Tang QY
,
Ivanova-Nikolova T
,
Mahajan R
,
Baki L
,
Zhang Z
,
Chavez J
,
Ubarretxena-Belandia I
,
Logothetis DE
.
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Kir3 channels control heart rate and neuronal excitability through GTP-binding (G) protein and phosphoinositide signaling pathways. These channels were the first characterized effectors of the βγ subunits of G proteins. Because we currently lack structures of complexes between G proteins and Kir3 channels, their interactions leading to modulation of channel function are not well understood. The recent crystal structure of a chimera between the cytosolic domain of a mammalian Kir3.1 and the transmembrane region of a prokaryotic KirBac1.3 (Kir3.1 chimera) has provided invaluable structural insight. However, it was not known whether this chimera could form functional K(+) channels. Here, we achieved the functional reconstitution of purified Kir3.1 chimera in planar lipid bilayers. The chimera behaved like a bona fide Kir channel displaying an absolute requirement for PIP(2) and Mg(2+)-dependent inward rectification. The channel could also be blocked by external tertiapin Q. The three-dimensional reconstruction of the chimera by single particle electron microscopy revealed a structure consistent with the crystal structure. Channel activity could be stimulated by ethanol and activated G proteins. Remarkably, the presence of both activated Gα and Gβγ subunits was required for gating of the channel. These results confirm the Kir3.1 chimera as a valid structural and functional model of Kir3 channels.
Aleksandrov,
Inward rectification of the IRK1 K+ channel reconstituted in lipid bilayers.
1996, Pubmed,
Xenbase
Aleksandrov,
Inward rectification of the IRK1 K+ channel reconstituted in lipid bilayers.
1996,
Pubmed
,
Xenbase
Aryal,
A discrete alcohol pocket involved in GIRK channel activation.
2009,
Pubmed
Chan,
Control of channel activity through a unique amino acid residue of a G protein-gated inwardly rectifying K+ channel subunit.
1996,
Pubmed
,
Xenbase
Chan,
A recombinant inwardly rectifying potassium channel coupled to GTP-binding proteins.
1996,
Pubmed
,
Xenbase
Dascal,
Expression of an atrial G-protein-activated potassium channel in Xenopus oocytes.
1993,
Pubmed
,
Xenbase
Doyle,
The structure of the potassium channel: molecular basis of K+ conduction and selectivity.
1998,
Pubmed
Gao,
Cloning and expression of a widely distributed (type IV) adenylyl cyclase.
1991,
Pubmed
Gaudet,
Crystal structure at 2.4 angstroms resolution of the complex of transducin betagamma and its regulator, phosducin.
1996,
Pubmed
He,
Identification of critical residues controlling G protein-gated inwardly rectifying K(+) channel activity through interactions with the beta gamma subunits of G proteins.
2002,
Pubmed
He,
Identification of a potassium channel site that interacts with G protein betagamma subunits to mediate agonist-induced signaling.
1999,
Pubmed
Herlitze,
Modulation of Ca2+ channels by G-protein beta gamma subunits.
1996,
Pubmed
Heymann,
Bsoft: image and molecular processing in electron microscopy.
2001,
Pubmed
Hibino,
Inwardly rectifying potassium channels: their structure, function, and physiological roles.
2010,
Pubmed
Huang,
Direct activation of inward rectifier potassium channels by PIP2 and its stabilization by Gbetagamma.
1998,
Pubmed
,
Xenbase
Ikeda,
Voltage-dependent modulation of N-type calcium channels by G-protein beta gamma subunits.
1996,
Pubmed
Inanobe,
Structural diversity in the cytoplasmic region of G protein-gated inward rectifier K+ channels.
2007,
Pubmed
Jin,
Mechanisms of inward-rectifier K+ channel inhibition by tertiapin-Q.
1999,
Pubmed
,
Xenbase
Jin,
A novel high-affinity inhibitor for inward-rectifier K+ channels.
1998,
Pubmed
,
Xenbase
Jin,
Synthesis of a stable form of tertiapin: a high-affinity inhibitor for inward-rectifier K+ channels.
1999,
Pubmed
,
Xenbase
Kobayashi,
Ethanol opens G-protein-activated inwardly rectifying K+ channels.
1999,
Pubmed
,
Xenbase
Krapivinsky,
The G-protein-gated atrial K+ channel IKACh is a heteromultimer of two inwardly rectifying K(+)-channel proteins.
1995,
Pubmed
,
Xenbase
Kubo,
Primary structure and functional expression of a rat G-protein-coupled muscarinic potassium channel.
1993,
Pubmed
,
Xenbase
Kuo,
Two different conformational states of the KirBac3.1 potassium channel revealed by electron crystallography.
2005,
Pubmed
Kuo,
Crystal structure of the potassium channel KirBac1.1 in the closed state.
2003,
Pubmed
Leal-Pinto,
Molecular cloning and functional reconstitution of a urate transporter/channel.
1997,
Pubmed
Leal-Pinto,
Reconstitution of hepatic uricase in planar lipid bilayer reveals a functional organic anion channel.
1995,
Pubmed
Lesage,
Molecular properties of neuronal G-protein-activated inwardly rectifying K+ channels.
1995,
Pubmed
,
Xenbase
Lesage,
Cloning provides evidence for a family of inward rectifier and G-protein coupled K+ channels in the brain.
1994,
Pubmed
,
Xenbase
Lewohl,
G-protein-coupled inwardly rectifying potassium channels are targets of alcohol action.
1999,
Pubmed
,
Xenbase
Logothetis,
Dynamic changes in phosphoinositide levels control ion channel activity.
2007,
Pubmed
Logothetis,
Channelopathies linked to plasma membrane phosphoinositides.
2010,
Pubmed
Logothetis,
Phosphoinositide-mediated gating of inwardly rectifying K(+) channels.
2007,
Pubmed
Logothetis,
Diverse Kir modulators act in close proximity to residues implicated in phosphoinositide binding.
2007,
Pubmed
Logothetis,
The beta gamma subunits of GTP-binding proteins activate the muscarinic K+ channel in heart.
,
Pubmed
Lopes,
Alterations in conserved Kir channel-PIP2 interactions underlie channelopathies.
2002,
Pubmed
,
Xenbase
Ludtke,
EMAN: semiautomated software for high-resolution single-particle reconstructions.
1999,
Pubmed
Marabini,
Xmipp: An Image Processing Package for Electron Microscopy.
1996,
Pubmed
Mindell,
Accurate determination of local defocus and specimen tilt in electron microscopy.
2003,
Pubmed
Mirshahi,
Molecular determinants responsible for differential cellular distribution of G protein-gated inwardly rectifying K+ channels.
2004,
Pubmed
,
Xenbase
Muench,
Cryo-electron microscopy of the vacuolar ATPase motor reveals its mechanical and regulatory complexity.
2009,
Pubmed
Nishida,
Structural basis of inward rectification: cytoplasmic pore of the G protein-gated inward rectifier GIRK1 at 1.8 A resolution.
2002,
Pubmed
Nishida,
Crystal structure of a Kir3.1-prokaryotic Kir channel chimera.
2007,
Pubmed
Pegan,
Cytoplasmic domain structures of Kir2.1 and Kir3.1 show sites for modulating gating and rectification.
2005,
Pubmed
,
Xenbase
Pettersen,
UCSF Chimera--a visualization system for exploratory research and analysis.
2004,
Pubmed
Reuveny,
Activation of the cloned muscarinic potassium channel by G protein beta gamma subunits.
1994,
Pubmed
,
Xenbase
Rohács,
Distinct specificities of inwardly rectifying K(+) channels for phosphoinositides.
1999,
Pubmed
,
Xenbase
Rostovtseva,
Voltage gating of VDAC is regulated by nonlamellar lipids of mitochondrial membranes.
2006,
Pubmed
Rubinstein,
Galphai3 primes the G protein-activated K+ channels for activation by coexpressed Gbetagamma in intact Xenopus oocytes.
2007,
Pubmed
,
Xenbase
Rubinstein,
Structural analysis of membrane protein complexes by single particle electron microscopy.
2007,
Pubmed
Scheres,
Modeling experimental image formation for likelihood-based classification of electron microscopy data.
2007,
Pubmed
Scheres,
Image processing for electron microscopy single-particle analysis using XMIPP.
2008,
Pubmed
Schreibmayer,
Inhibition of an inwardly rectifying K+ channel by G-protein alpha-subunits.
1996,
Pubmed
,
Xenbase
Stanfield,
Constitutively active and G-protein coupled inward rectifier K+ channels: Kir2.0 and Kir3.0.
2002,
Pubmed
Stansfeld,
PIP(2)-binding site in Kir channels: definition by multiscale biomolecular simulations.
2009,
Pubmed
Sui,
Activation of the atrial KACh channel by the betagamma subunits of G proteins or intracellular Na+ ions depends on the presence of phosphatidylinositol phosphates.
1998,
Pubmed
,
Xenbase
Tang,
Type-specific regulation of adenylyl cyclase by G protein beta gamma subunits.
1991,
Pubmed
Tao,
Crystal structure of the eukaryotic strong inward-rectifier K+ channel Kir2.2 at 3.1 A resolution.
2009,
Pubmed
,
Xenbase
Vivaudou,
Probing the G-protein regulation of GIRK1 and GIRK4, the two subunits of the KACh channel, using functional homomeric mutants.
1997,
Pubmed
,
Xenbase
Yamada,
G protein regulation of potassium ion channels.
1998,
Pubmed
