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Genes Dev
1998 Aug 01;1215:2345-53. doi: 10.1101/gad.12.15.2345.
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A novel homeobox gene, dharma, can induce the organizer in a non-cell-autonomous manner.
Yamanaka Y
,
Mizuno T
,
Sasai Y
,
Kishi M
,
Takeda H
,
Kim CH
,
Hibi M
,
Hirano T
.
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The formation of Spemann organizer is one of the most important steps in dorsoventral axis determination in vertebrate development. However, whether the organizer forms autonomously or is induced non-cell-autonomously is controversial. In this report we have isolated a novel zebrafish homeobox gene, dharma, capable of inducing the organizer ectopically. The expression of dharma was first detected in several blastomeres at one side of the margin soon after the mid-blastula transition and continued in the dorsal side of the yolk syncytial layer (YSL) under the embryonic shield, the zebrafish organizer, until the onset of gastrulation. Furthermore, dharma expressed in the YSL induced the organizer in a non-cell-autonomous manner. These results provided the first identification of a zygotic gene to be implicated in the formation of an organizer-inducing center.
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9694799
???displayArticle.pmcLink???PMC317041 ???displayArticle.link???Genes Dev
Figure 1.
 Phenotypes of dharma mRNA-injected embryos. (A) dharma mRNA (5 pg)-injected embryos at 48 pfh. (Top) Normal embryo at 48 pfh. (Bottom)dharma mRNA-injected embryos with the Snh-like phenotype. (B–D) dharma RNA (12.5 pg)-injected embryos. (B) An embryo with football shape at 12 pfh. (C) An embryotail malformation at 24 pfh. (D) An embryo Snh-like phenotype at 24 pfh (dorsal view). (E) The double-axis phenotype in the Xenopus embryo induced by dharmaexpression. One-hundred picograms of dharma mRNA was injected into the ventral–vegetal cell with 40 pg of β-galactosidase mRNA. (White arrow head) Second axis. Out of 24 injected embryos, 9 embryos were normal, 11 contained double axes, 2 were dorsalized, and 2 had spina bifida. Of the 11 double-axis embryos, 9 exhibited X-gal staining only in the endoderm, and 2 exhibited X-gal staining in both the endoderm and the second axis. Control embryos injected with 100 pg of β-gal RNA were normal (data not shown).
Bauer,
The cleavage stage origin of Spemann's Organizer: analysis of the movements of blastomere clones before and during gastrulation in Xenopus.
1994, Pubmed,
Xenbase
Bauer,
The cleavage stage origin of Spemann's Organizer: analysis of the movements of blastomere clones before and during gastrulation in Xenopus.
1994,
Pubmed
,
Xenbase
Behrens,
Functional interaction of beta-catenin with the transcription factor LEF-1.
1996,
Pubmed
,
Xenbase
Boterenbrood,
The formation of the mesoderm in urodelean amphibians : V. Its regional induction by the endoderm.
1973,
Pubmed
Cadigan,
Wnt signaling: a common theme in animal development.
1997,
Pubmed
,
Xenbase
Cho,
Molecular nature of Spemann's organizer: the role of the Xenopus homeobox gene goosecoid.
1991,
Pubmed
,
Xenbase
Cooper,
A cluster of noninvoluting endocytic cells at the margin of the zebrafish blastoderm marks the site of embryonic shield formation.
1996,
Pubmed
D'Amico,
Spatially distinct domains of cell behavior in the zebrafish organizer region.
1997,
Pubmed
,
Xenbase
Darras,
Animal and vegetal pole cells of early Xenopus embryos respond differently to maternal dorsal determinants: implications for the patterning of the organiser.
1997,
Pubmed
,
Xenbase
Driever,
Axis formation in zebrafish.
1995,
Pubmed
,
Xenbase
Gallagher,
Autonomous differentiation of dorsal axial structures from an animal cap cleavage stage blastomere in Xenopus.
1991,
Pubmed
,
Xenbase
Gimlich,
Early cellular interactions promote embryonic axis formation in Xenopus laevis.
1984,
Pubmed
,
Xenbase
Gimlich,
Acquisition of developmental autonomy in the equatorial region of the Xenopus embryo.
1986,
Pubmed
,
Xenbase
Hammerschmidt,
Genetic analysis of dorsoventral pattern formation in the zebrafish: requirement of a BMP-like ventralizing activity and its dorsal repressor.
1996,
Pubmed
Heasman,
Patterning the Xenopus blastula.
1997,
Pubmed
,
Xenbase
Jowett,
Whole-mount in situ hybridizations on zebrafish embryos using a mixture of digoxigenin- and fluorescein-labelled probes.
1994,
Pubmed
Kageura,
Spatial distribution of the capacity to initiate a secondary embryo in the 32-cell embryo of Xenopus laevis.
1990,
Pubmed
,
Xenbase
Klein,
A molecular mechanism for the effect of lithium on development.
1996,
Pubmed
,
Xenbase
Larabell,
Establishment of the dorso-ventral axis in Xenopus embryos is presaged by early asymmetries in beta-catenin that are modulated by the Wnt signaling pathway.
1997,
Pubmed
,
Xenbase
Laurent,
The Xenopus homeobox gene twin mediates Wnt induction of goosecoid in establishment of Spemann's organizer.
1997,
Pubmed
,
Xenbase
Lemaire,
A role for cytoplasmic determinants in mesoderm patterning: cell-autonomous activation of the goosecoid and Xwnt-8 genes along the dorsoventral axis of early Xenopus embryos.
1994,
Pubmed
,
Xenbase
Lemaire,
The vertebrate organizer: structure and molecules.
1996,
Pubmed
,
Xenbase
Lemaire,
Expression cloning of Siamois, a Xenopus homeobox gene expressed in dorsal-vegetal cells of blastulae and able to induce a complete secondary axis.
1995,
Pubmed
,
Xenbase
Molenaar,
XTcf-3 transcription factor mediates beta-catenin-induced axis formation in Xenopus embryos.
1996,
Pubmed
,
Xenbase
Moon,
WNTs modulate cell fate and behavior during vertebrate development.
1997,
Pubmed
Moon,
Structurally related receptors and antagonists compete for secreted Wnt ligands.
1997,
Pubmed
,
Xenbase
Mullins,
Genes establishing dorsoventral pattern formation in the zebrafish embryo: the ventral specifying genes.
1996,
Pubmed
Nieuwkoop,
The formation of the mesoderm in urodelean amphibians : I. Induction by the endoderm.
1969,
Pubmed
Sagerström,
Anteroposterior patterning in the zebrafish, Danio rerio: an explant assay reveals inductive and suppressive cell interactions.
1996,
Pubmed
Sakai,
The vegetal determinants required for the Spemann organizer move equatorially during the first cell cycle.
1996,
Pubmed
,
Xenbase
Sasai,
Ectodermal patterning in vertebrate embryos.
1997,
Pubmed
,
Xenbase
Schneider,
Beta-catenin translocation into nuclei demarcates the dorsalizing centers in frog and fish embryos.
1996,
Pubmed
,
Xenbase
Schulte-Merker,
Expression of zebrafish goosecoid and no tail gene products in wild-type and mutant no tail embryos.
1994,
Pubmed
,
Xenbase
Schulte-Merker,
The protein product of the zebrafish homologue of the mouse T gene is expressed in nuclei of the germ ring and the notochord of the early embryo.
1992,
Pubmed
Slack,
Inducing factors in Xenopus early embryos.
1994,
Pubmed
,
Xenbase
Smith,
Expression cloning of noggin, a new dorsalizing factor localized to the Spemann organizer in Xenopus embryos.
1992,
Pubmed
,
Xenbase
Smith,
Injected Xwnt-8 RNA acts early in Xenopus embryos to promote formation of a vegetal dorsalizing center.
1991,
Pubmed
,
Xenbase
Stachel,
Lithium perturbation and goosecoid expression identify a dorsal specification pathway in the pregastrula zebrafish.
1993,
Pubmed
Taira,
The LIM domain-containing homeo box gene Xlim-1 is expressed specifically in the organizer region of Xenopus gastrula embryos.
1992,
Pubmed
,
Xenbase
Takasaki,
Fates and Roles of the Presumptive Organizer Region in the 32-cell Embryo in Normal Development of Xenopus laevis.
1987,
Pubmed
,
Xenbase
Wylie,
Maternal beta-catenin establishes a 'dorsal signal' in early Xenopus embryos.
1996,
Pubmed
,
Xenbase
