Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Functional activity and chromatin configuration of SV40 enhancer injected in Xenopus laevis oocytes.
Spinelli G
,
Ciliberto G
.
???displayArticle.abstract???
The SV40 enhancer microinjected into the nuclei of X. laevis oocytes is able to activate transcription about 100-fold when cloned upstream of the SV40 early promoter region or about 10-fold regardless of its orientation when located at a long distance from a test gene (bacterial chloramphenicol acetyl transferase). This effect is qualitatively and quantitatively similar to that observed when analogous constructions were transfected into mammalian cells. Making use of a direct labelling technique for the analysis of the chromatin structure we could show that the SV40 enhancer region in the microinjected plasmids is particularly accessible to cleavage with MNase or DNAse I. Single sites in the 72 bp repeats have been mapped at the nucleotide level.
Alton,
Nucleotide sequence analysis of the chloramphenicol resistance transposon Tn9.
, Pubmed
Alton,
Nucleotide sequence analysis of the chloramphenicol resistance transposon Tn9.
,
Pubmed
Augereau,
The MLV and SV40 enhancers have a similar pattern of transcriptional activation.
1984,
Pubmed
Banerji,
Expression of a beta-globin gene is enhanced by remote SV40 DNA sequences.
1981,
Pubmed
Banerji,
A lymphocyte-specific cellular enhancer is located downstream of the joining region in immunoglobulin heavy chain genes.
1983,
Pubmed
Berk,
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
1977,
Pubmed
Cereghini,
Assembly of transfected DNA into chromatin: structural changes in the origin-promoter-enhancer region upon replication.
1984,
Pubmed
Cremisi,
The appearance of DNase I hypersensitive sites at the 5' end of the late SV40 genes is correlated with the transcriptional switch.
1981,
Pubmed
de Villiers,
A small segment of polyoma virus DNA enhances the expression of a cloned beta-globin gene over a distance of 1400 base pairs.
1981,
Pubmed
de Villiers,
Transcriptional 'enhancers' from SV40 and polyoma virus show a cell type preference.
1982,
Pubmed
Fromm,
Simian virus 40 early- and late-region promoter functions are enhanced by the 72-base-pair repeat inserted at distant locations and inverted orientations.
1983,
Pubmed
Gargiulo,
Analysis of the chromatin assembled in germinal vesicles of Xenopus oocytes.
1983,
Pubmed
,
Xenbase
Gillies,
A tissue-specific transcription enhancer element is located in the major intron of a rearranged immunoglobulin heavy chain gene.
1983,
Pubmed
Gorman,
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
1982,
Pubmed
Gruss,
Magic enhancers?
1984,
Pubmed
Gurdon,
The use of Xenopus oocytes for the expression of cloned genes.
1983,
Pubmed
,
Xenbase
Harland,
Regulated replication of DNA microinjected into eggs of Xenopus laevis.
1980,
Pubmed
,
Xenbase
Herbomel,
Fine structure of the origin-proximal DNAase I-hypersensitive region in wild-type and EC mutant polyoma.
1981,
Pubmed
Innis,
Chromatin structure of simian virus 40-pBR322 recombinant plasmids in COS-1 cells.
1983,
Pubmed
Jakobovits,
A nucleosome-free region in SV40 minichromosomes.
1980,
Pubmed
Jongstra,
Induction of altered chromatin structures by simian virus 40 enhancer and promoter elements.
,
Pubmed
Khoury,
Enhancer elements.
1983,
Pubmed
Laimins,
Host-specific activation of transcription by tandem repeats from simian virus 40 and Moloney murine sarcoma virus.
1982,
Pubmed
Messing,
New M13 vectors for cloning.
1983,
Pubmed
Moreau,
The SV40 72 base repair repeat has a striking effect on gene expression both in SV40 and other chimeric recombinants.
1981,
Pubmed
Mosthaf,
A viral enhancer element specifically active in human haematopoietic cells.
,
Pubmed
Queen,
Immunoglobulin gene transcription is activated by downstream sequence elements.
1983,
Pubmed
Saragosti,
Absence of nucleosomes in a fraction of SV40 chromatin between the origin of replication and the region coding for the late leader RNA.
1980,
Pubmed
Sassone-Corsi,
A trans-acting factor is responsible for the simian virus 40 enhancer activity in vitro.
,
Pubmed
Sassone-Corsi,
Stimulation of in vitro transcription from heterologous promoters by the simian virus 40 enhancer.
1984,
Pubmed
Schöler,
Specific interaction between enhancer-containing molecules and cellular components.
1984,
Pubmed
Scott,
Sites in simian virus 40 chromatin which are preferentially cleaved by endonucleases.
1978,
Pubmed
Treisman,
Simian virus 40 enhancer increases number of RNA polymerase II molecules on linked DNA.
,
Pubmed
Trendelenburg,
Transcription of cloned Xenopus ribosomal genes visualised after injection into oocyte nuclei.
1978,
Pubmed
,
Xenbase
Varshavsky,
A stretch of "late" SV40 viral DNA about 400 bp long which includes the origin of replication is specifically exposed in SV40 minichromosomes.
1979,
Pubmed
Walker,
Cell-specific expression controlled by the 5'-flanking region of insulin and chymotrypsin genes.
,
Pubmed
Wasylyk,
The SV40 72 bp repeat preferentially potentiates transcription starting from proximal natural or substitute promoter elements.
1983,
Pubmed
Weber,
Simian virus 40 enhancer increases RNA polymerase density within the linked gene.
,
Pubmed
Wyllie,
Selective DNA conservation and chromatin assembly after injection of SV40 DNA into Xenopus oocytes.
1978,
Pubmed
,
Xenbase
