Zhang JY et al. (1999), A novel cytoplasmic protein with RNA-binding mo...

XB-ART-13255

J Exp Med 1999 Apr 05;1897:1101-10. doi: 10.1084/jem.189.7.1101.

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A novel cytoplasmic protein with RNA-binding motifs is an autoantigen in human hepatocellular carcinoma.

Zhang JY , Chan EK , Peng XX , Tan EM .

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In hepatocellular carcinoma (HCC), autoantibodies to intracellular antigens are detected in 30-40% of patients. Patients with chronic hepatitis or liver cirrhosis develop HCC, and when this occurs, some patients exhibit autoantibodies of new specificities. It has been suggested that these novel autoantibody responses may be immune system reactions to proteins involved in transformation-associated cellular events. One HCC serum shown to contain antibodies to unidentified cellular antigens was used to immunoscreen a cDNA expression library, and a full length cDNA clone was isolated with an open reading frame encoding 556 amino acids with a predicted molecular mass of 62 kD. The 62-kD protein contained two types of RNA-binding motifs, the consensus sequence RNA-binding domain (CS-RBD) and four hnRNP K homology (KH) domains. This protein, provisionally called p62, has close identity (66-70%) to three other proteins at the amino acid sequence level, and all four proteins may belong to a family having CS-RBD in the NH2-terminal region and four KH domains in the mid-to-COOH- terminal region. The homologous proteins are: KH domain-containing protein overexpressed in cancer (Koc); zipcode binding protein, a protein which binds to a conserved nucleotide element in chicken beta-actin mRNA (ZBP1); and a protein which binds to a promoter cis element in Xenopus laevis TFIIIA gene (B3). p62 protein is cytoplasmic in location, and autoantibodies were found in 21% of a cohort of HCC patients. Patients with chronic hepatitis and liver cirrhosis, conditions which are frequent precursors to HCC, were negative for these autoantibodies, suggesting that the immune response might be related to cellular events leading to transformation. However, the possible involvement of p62 autoantigen as a factor in the transformation process remains to be elucidated.

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Species referenced: Xenopus laevis
Genes referenced: actb actl6a gtf3a hnrnpc igf2bp3 nup62 sqstm1 ssb zbp1

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	Figure 1. Reactivity of three HCC sera in Western blotting against whole cell extracts from MOLT-4, T24, and HepG2 cell lines. Lanes 1, 5, and 9 were normal human sera. Serum YZ (lanes 2, 6, and 10) showed strong reactivity with a 90-kD protein in MOLT-4 cells and reacted weakly with a 62-kD protein in MOLT-4 cells and T24 cell extracts. A strong reaction with the 62-kD protein was detected with HepG2 cell extracts, together with a strong reaction with a 50-kD protein. Sera YL (lanes 3, 7, and 11) and CH (lanes 4, 8, and 12) demonstrate other types of reactions (see text). These representative data demonstrate that HCC sera are heterogeneous in their antibody repertoires and that different cell lines apparently have different expressions of 90-, 62-, and 50-kD proteins.
	Figure 3. Similarity between p62 and three RNA-binding motif proteins Koc, ZBP1, and B3. (A) The identity between p62 and the other three proteins are shown (*). (B) The great similarity of the domain structures is shown. The RNA-binding domains with two conserved RNP regions, RNP1 and RNP2, and four KH domains are boxed. A nineâamino acid sequence (VGAIIGKE/KG) of unknown function in the first three KH domains is shown in bold as is the REV-like nuclear export signal in the second KH domain.
	Figure 4. p62 recombinant protein expressed as a 6Ã histidine tag protein in E. coli was purified using nickel column chromatography. (A) Molecular mass markers (lane M). A 62-kD peptide that corresponded to the size of the ORF of p62 was detected in SDS-PAGE with Coomassie blue staining (lane 1). HCC serum YZ, which was used for cDNA cloning, was reactive with p62 recombinant protein in Western blot analysis (lane 3), but the recombinant protein was not reactive with normal human serum (lane 2) or HCC sera that did not contain detectable antibodies to the 62-kD protein (not shown). A lower molecular size product, presumably a degradation product, detectable by Coomassie blue staining was also reactive with HCC serum (lanes 1 and 3). (B) Immunoprecipitation of in vitroâtranslated p62 cDNA. Anti-p62 prototype serum (lane 4), normal human serum (lane 5), and in vitroâtranslated products alone (lane 6). (C) Comigration of the in vitroâtranslated products of p62 with the 62-kD cellular protein in HepG2 cells. The panel (lanes 7â9) was processed by autoradiography and the same panel (7aâ9a) used subsequently for Western blot analysis. Lanes 7/7a contained HepG2 cell extracts alone, lanes 8/8a contained a mixture of in vitroâtranslated radiolabeled products and HepG2 cell extracts, and lanes 9/9a contained in vitroâtranslated radiolabeled products alone. The in vitroâtranslated radiolabeled product of p62 cDNA comigrated exactly with cellular p62.
	Figure 5. Human antiâ62-kD antibody and rabbit antiârecombinant p62 antibodies recognize recombinant antigen. (A) Immunoprecipitation using in vitroâtranslated products of p62. Lane 1, in vitroâtranslated products; lane 2, preimmune rabbit serum (No. 3366); lane 3, immune rabbit serum (No. 3366). (B) Immunoprecipitation using [35S]methionineâlabeled HeLa cell extracts. Lane 4, normal human serum; lane 5, anti-SSB/La prototype serum, which recognizes an irrelevant 48-kD autoantigen used as a positive control for the assay; lane 6, HCC serum without p62 autoantibodies; lanes 7 and 8, p62 prototype sera; lane 9, preimmune rabbit serum (No. 3366); lane 10, immune rabbit serum (No. 3366).
	Figure 6. p62 is a cytoplasmic protein, as revealed by immunofluorescence analysis of HEp-2 cell substrate using human p62 prototype serum YZ (A) and affinity-purified rabbit antiâp62 serum No. 3366 (B).
	Figure 7. Northern blot analysis of p62 mRNA. The nylon membrane blotted with poly A+ RNA isolated from multiple human tissues (A) and human cancer cell lines (B) was purchased from Clontech. Each lane contained 2 Î¼g of purified poly A+ RNA. An antisense riboprobe was generated from the 480-bp fragments corresponding to the COOH-terminal domain of p62 (see Fig. 2 A) and hybridized to the membrane at a concentration of 1.0 Ã 106 cpm of the probe per milliliter of hybridization solution. Two transcripts of 3.7 and 5.2 kb were detected in human tissues (A, arrows) and cancer cell lines (B, arrows). The 3.7-kb transcript of p62 was found in heart and placenta, but its occurrence was lower or negative in brain, lung, liver, kidney, and pancreas. A signal for skeletal muscle migrated somewhat more slowly than the 3.7-kb transcript (see text). High levels of the 3.7-kb transcript were detected in HeLa, K-562, SW480, A549, and G361 cell lines, but low expression was observed in HL-60, MOLT-4, and Raji cell lines. The 3.7-kb transcript expression corresponded to detectable cellular p62 protein (see text), but the significance of the 5.2-kb RNA is unknown. A 2.0-kb human Î²-actin cDNA probe was used as a control for monitoring RNA levels. As described in the manufacturer's (Clontech) instructions, there are two forms of Î²-actin mRNA, a 2- and 1.6â1.8-kb form, in heart and skeletal muscle.

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Bravo, Cyclin/PCNA is the auxiliary protein of DNA polymerase-delta. , Pubmed