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Autoinduction of activin genes in early Xenopus embryos.
Suzuki A
,
Nagai T
,
Nishimatsu S
,
Sugino H
,
Eto Y
,
Shibai H
,
Murakami K
,
Ueno N
.
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Activin exhibits a potent mesoderm inducing activity towards the ectodermal tissue (animal cap) of Xenopus laevis blastulae. Thus in order to investigate the role of activin in morphogenesis of early Xenopus embryos, activation of genes for activin beta A and beta B was examined by the reverse transcription polymerase chain reaction. In vivo, activin beta B mRNA appears to be present in embryonic stage 1 whereas beta A mRNA is undetectable prior to gastrulation. beta B and beta A mRNAs were noted to accumulate after stages 9 and 15 respectively. Activin gene expression in Xenopus animal caps was examined after treatment with various concentrations of activin A. Under these treatment conditions, both activin beta A and beta B mRNAs accumulated in a dose-dependent fashion after 24 h. The same effect was noted for treatment with similar concentrations of activin B. Accumulation of mRNAs was inhibited by the addition of cycloheximide to the culture medium, consistent with the proposition that activin gene expression requires certain protein factors. In total, therefore, these data suggest that an autoinduction mechanism is involved in the regulation of activin mRNA levels in normal Xenopus embryos and that this mechanism may play a pivotal role during early embryonic development.
Asashima,
Presence of activin (erythroid differentiation factor) in unfertilized eggs and blastulae of Xenopus laevis.
1991, Pubmed,
Xenbase
Asashima,
Presence of activin (erythroid differentiation factor) in unfertilized eggs and blastulae of Xenopus laevis.
1991,
Pubmed
,
Xenbase
Cho,
Molecular nature of Spemann's organizer: the role of the Xenopus homeobox gene goosecoid.
1991,
Pubmed
,
Xenbase
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Coffey,
Production and auto-induction of transforming growth factor-alpha in human keratinocytes.
,
Pubmed
Dirksen,
A novel, activin-inducible, blastopore lip-specific gene of Xenopus laevis contains a fork head DNA-binding domain.
1992,
Pubmed
,
Xenbase
Dohrmann,
Expression of activin mRNA during early development in Xenopus laevis.
1993,
Pubmed
,
Xenbase
Durston,
Retinoic acid causes an anteroposterior transformation in the developing central nervous system.
1989,
Pubmed
,
Xenbase
Ellinger-Ziegelbauer,
A retinoic acid receptor expressed in the early development of Xenopus laevis.
1991,
Pubmed
,
Xenbase
Eto,
Purification and characterization of erythroid differentiation factor (EDF) isolated from human leukemia cell line THP-1.
1987,
Pubmed
Fujimoto,
Purification of megakaryocyte differentiation activity from a human fibrous histiocytoma cell line: N-terminal sequence homology with activin A.
1991,
Pubmed
Green,
Graded changes in dose of a Xenopus activin A homologue elicit stepwise transitions in embryonic cell fate.
1990,
Pubmed
,
Xenbase
Green,
The biological effects of XTC-MIF: quantitative comparison with Xenopus bFGF.
1990,
Pubmed
,
Xenbase
Hashimoto,
Activin/EDF as an inhibitor of neural differentiation.
1990,
Pubmed
Hashimoto,
Functional regulation of osteoblastic cells by the interaction of activin-A with follistatin.
1992,
Pubmed
Krieg,
The mRNA encoding elongation factor 1-alpha (EF-1 alpha) is a major transcript at the midblastula transition in Xenopus.
1989,
Pubmed
,
Xenbase
Ling,
Pituitary FSH is released by a heterodimer of the beta-subunits from the two forms of inhibin.
,
Pubmed
Mason,
Complementary DNA sequences of ovarian follicular fluid inhibin show precursor structure and homology with transforming growth factor-beta.
,
Pubmed
Mason,
Activin B: precursor sequences, genomic structure and in vitro activities.
1989,
Pubmed
Massagué,
The TGF-beta family of growth and differentiation factors.
1987,
Pubmed
Mathews,
Cloning of a second type of activin receptor and functional characterization in Xenopus embryos.
1992,
Pubmed
,
Xenbase
Melton,
Pattern formation during animal development.
1991,
Pubmed
,
Xenbase
Murata,
Erythroid differentiation factor is encoded by the same mRNA as that of the inhibin beta A chain.
1988,
Pubmed
Nakamura,
Isolation and characterization of native activin B.
1992,
Pubmed
,
Xenbase
Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1982,
Pubmed
,
Xenbase
Nishimatsu,
Multiple genes for Xenopus activin receptor expressed during early embryogenesis.
1992,
Pubmed
,
Xenbase
Nishimatsu,
A carboxyl-terminal truncated version of the activin receptor mediates activin signals in early Xenopus embryos.
1992,
Pubmed
,
Xenbase
Ogawa,
Bovine bone activin enhances bone morphogenetic protein-induced ectopic bone formation.
1992,
Pubmed
Paulsson,
Possible positive autocrine feedback in the prereplicative phase of human fibroblasts.
,
Pubmed
Rosa,
Mix.1, a homeobox mRNA inducible by mesoderm inducers, is expressed mostly in the presumptive endodermal cells of Xenopus embryos.
1989,
Pubmed
,
Xenbase
Ruiz i Altaba,
Retinoic acid modifies the pattern of cell differentiation in the central nervous system of neurula stage Xenopus embryos.
1991,
Pubmed
,
Xenbase
Ruiz i Altaba,
Retinoic acid modifies mesodermal patterning in early Xenopus embryos.
1991,
Pubmed
,
Xenbase
Rupp,
Ubiquitous MyoD transcription at the midblastula transition precedes induction-dependent MyoD expression in presumptive mesoderm of X. laevis.
1991,
Pubmed
,
Xenbase
Sanger,
Determination of nucleotide sequences in DNA.
1981,
Pubmed
Schubert,
Activin is a nerve cell survival molecule.
1990,
Pubmed
Sive,
Identification of a retinoic acid-sensitive period during primary axis formation in Xenopus laevis.
1990,
Pubmed
,
Xenbase
Smith,
Identification of a potent Xenopus mesoderm-inducing factor as a homologue of activin A.
1990,
Pubmed
,
Xenbase
Suzuki,
Differential expression of Xenopus BMPs in early embryos and tissues.
1993,
Pubmed
,
Xenbase
Tabin,
Retinoids, homeoboxes, and growth factors: toward molecular models for limb development.
1991,
Pubmed
Taira,
The LIM domain-containing homeo box gene Xlim-1 is expressed specifically in the organizer region of Xenopus gastrula embryos.
1992,
Pubmed
,
Xenbase
Tashiro,
Expression of mRNA for activin-binding protein (follistatin) during early embryonic development of Xenopus laevis.
1991,
Pubmed
,
Xenbase
Thomsen,
Activins are expressed early in Xenopus embryogenesis and can induce axial mesoderm and anterior structures.
1990,
Pubmed
,
Xenbase
Tickle,
Local application of retinoic acid to the limb bond mimics the action of the polarizing region.
1982,
Pubmed
Ueno,
Activin as a cell differentiation factor.
1990,
Pubmed
,
Xenbase
Vale,
Purification and characterization of an FSH releasing protein from porcine ovarian follicular fluid.
,
Pubmed
van den Eijnden-Van Raaij,
Activin-like factor from a Xenopus laevis cell line responsible for mesoderm induction.
1990,
Pubmed
,
Xenbase
Van Obberghen-Schilling,
Transforming growth factor beta 1 positively regulates its own expression in normal and transformed cells.
1988,
Pubmed
