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XB-ART-299
Methods Mol Biol 2006 Jan 01;322:55-67. doi: 10.1007/978-1-59745-000-3_5.
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Oocyte expression with injection of purified T7 RNA polymerase.

Altafaj X , Joux N , Ronjat M , De Waard M .


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The Xenopus oocyte is a widely used system for protein expression. Investigators have had the choice between two different techniques: injection into the cytoplasm of in vitro transcribed complementary RNA (cRNA) or injection into the nucleus of complementary DNA (cDNA). We report on a third expression technique that is based on the combined injection of cDNA and purified T7 RNA polymerase directly into the cytoplasm of oocytes.

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???displayArticle.link??? Methods Mol Biol