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Mol Cell Biol
2002 Jun 01;2211:3653-62. doi: 10.1128/MCB.22.11.3653-3662.2002.
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Nucleosome remodeling by the human SWI/SNF complex requires transient global disruption of histone-DNA interactions.
Aoyagi S
,
Narlikar G
,
Zheng C
,
Sif S
,
Kingston RE
,
Hayes JJ
.
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We utilized a site-specific cross-linking technique to investigate the mechanism of nucleosome remodeling by hSWI/SNF. We found that a single cross-link between H2B and DNA virtually eliminates the accumulation of stably remodeled species as measured by restriction enzyme accessibility assays. However, cross-linking the histone octamer to nucleosomal DNA does not inhibit remodeling as monitored by DNase I digestion assays. Importantly, we found that the restriction enzyme-accessible species can be efficiently cross-linked after remodeling and that the accessible state does not require continued ATP hydrolysis. These results imply that the generation of stable remodeled states requires at least transient disruption of histone-DNA interactions throughout the nucleosome, while hSWI/SNF-catalyzed disruption of just local histone-DNA interactions yields less-stable remodeled states that still display an altered DNase I cleavage pattern. The implications of these results for models of the mechanism of SWI/SNF-catalyzed nucleosome remodeling are discussed.
Angelov,
Differential remodeling of the HIV-1 nucleosome upon transcription activators and SWI/SNF complex binding.
2000, Pubmed
Angelov,
Differential remodeling of the HIV-1 nucleosome upon transcription activators and SWI/SNF complex binding.
2000,
Pubmed
Bazett-Jones,
The SWI/SNF complex creates loop domains in DNA and polynucleosome arrays and can disrupt DNA-histone contacts within these domains.
1999,
Pubmed
Boyer,
Roles of the histone H2A-H2B dimers and the (H3-H4)(2) tetramer in nucleosome remodeling by the SWI-SNF complex.
2000,
Pubmed
Cairns,
RSC, an essential, abundant chromatin-remodeling complex.
1996,
Pubmed
Chafin,
Human DNA ligase I efficiently seals nicks in nucleosomes.
2000,
Pubmed
,
Xenbase
Côté,
Perturbation of nucleosome core structure by the SWI/SNF complex persists after its detachment, enhancing subsequent transcription factor binding.
1998,
Pubmed
Côté,
Stimulation of GAL4 derivative binding to nucleosomal DNA by the yeast SWI/SNF complex.
1994,
Pubmed
Gavin,
SWI/SNF chromatin remodeling requires changes in DNA topology.
2001,
Pubmed
Guyon,
Stability of a human SWI-SNF remodeled nucleosomal array.
2001,
Pubmed
Hansen,
Structure and function of the core histone N-termini: more than meets the eye.
1998,
Pubmed
Havas,
Generation of superhelical torsion by ATP-dependent chromatin remodeling activities.
2000,
Pubmed
,
Xenbase
Imbalzano,
Nucleosome disruption by human SWI/SNF is maintained in the absence of continued ATP hydrolysis.
1996,
Pubmed
Imbalzano,
Facilitated binding of TATA-binding protein to nucleosomal DNA.
1994,
Pubmed
Jaskelioff,
SWI-SNF-mediated nucleosome remodeling: role of histone octamer mobility in the persistence of the remodeled state.
2000,
Pubmed
Kingston,
ATP-dependent remodeling and acetylation as regulators of chromatin fluidity.
1999,
Pubmed
Kornberg,
Twenty-five years of the nucleosome, fundamental particle of the eukaryote chromosome.
1999,
Pubmed
Kwon,
Nucleosome disruption and enhancement of activator binding by a human SW1/SNF complex.
1994,
Pubmed
,
Xenbase
Längst,
ISWI induces nucleosome sliding on nicked DNA.
2001,
Pubmed
Lee,
The N-terminal tail of histone H2A binds to two distinct sites within the nucleosome core.
1997,
Pubmed
,
Xenbase
Lee,
hSWI/SNF disrupts interactions between the H2A N-terminal tail and nucleosomal DNA.
1999,
Pubmed
,
Xenbase
Logie,
Catalytic activity of the yeast SWI/SNF complex on reconstituted nucleosome arrays.
1997,
Pubmed
Lorch,
RSC unravels the nucleosome.
2001,
Pubmed
,
Xenbase
Luger,
Crystal structure of the nucleosome core particle at 2.8 A resolution.
1997,
Pubmed
Narlikar,
Generation and interconversion of multiple distinct nucleosomal states as a mechanism for catalyzing chromatin fluidity.
2001,
Pubmed
Peterson,
Characterization of the yeast SWI1, SWI2, and SWI3 genes, which encode a global activator of transcription.
1992,
Pubmed
Peterson,
Promoter targeting and chromatin remodeling by the SWI/SNF complex.
2000,
Pubmed
Peterson,
Multiple SWItches to turn on chromatin?
1996,
Pubmed
Polach,
Mechanism of protein access to specific DNA sequences in chromatin: a dynamic equilibrium model for gene regulation.
1995,
Pubmed
Schnitzler,
Direct imaging of human SWI/SNF-remodeled mono- and polynucleosomes by atomic force microscopy employing carbon nanotube tips.
2001,
Pubmed
Schnitzler,
Human SWI/SNF interconverts a nucleosome between its base state and a stable remodeled state.
1998,
Pubmed
Sengupta,
The interactions of yeast SWI/SNF and RSC with the nucleosome before and after chromatin remodeling.
2001,
Pubmed
Strahl,
The language of covalent histone modifications.
2000,
Pubmed
Tamkun,
brahma: a regulator of Drosophila homeotic genes structurally related to the yeast transcriptional activator SNF2/SWI2.
1992,
Pubmed
Vignali,
ATP-dependent chromatin-remodeling complexes.
2000,
Pubmed
Wade,
Transcriptional regulation: SWItching circuitry.
1999,
Pubmed
Whitehouse,
Nucleosome mobilization catalysed by the yeast SWI/SNF complex.
1999,
Pubmed
Winston,
Yeast SNF/SWI transcriptional activators and the SPT/SIN chromatin connection.
1992,
Pubmed
Wolffe,
Chromatin disruption and modification.
1999,
Pubmed
Workman,
Alteration of nucleosome structure as a mechanism of transcriptional regulation.
1998,
Pubmed
