Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1998 Jun 01;1711:3016-28. doi: 10.1093/emboj/17.11.3016.
Show Gene links
Show Anatomy links
P2X1 and P2X3 receptors form stable trimers: a novel structural motif of ligand-gated ion channels.
Nicke A
,
Bäumert HG
,
Rettinger J
,
Eichele A
,
Lambrecht G
,
Mutschler E
,
Schmalzing G
.
???displayArticle.abstract???
P2X receptors are cation channels gated by extracellular ATP. The seven known P2X isoforms possess no sequence homology with other proteins. Here we studied the quaternary structure of P2X receptors by chemical cross-linking and blue native PAGE. P2X1 and P2X3 were N-terminally tagged with six histidine residues to allow for non-denaturing receptor isolation from cRNA-injected, [35S]methionine-labeled oocytes. The His-tag did not change the electrophysiological properties of the P2X1 receptor. His-P2X1 was found to carry four N-glycans per polypeptide chain, only one of which acquired Endo H resistance en route to the plasma membrane. 3, 3'-Dithiobis(sulfosuccinimidylpropionate) (DTSSP) and two of three bifunctional analogues of the P2X receptor antagonist pyridoxalphosphate-6-azophenyl-2',4'-disulfonic acid (PPADS) cross-linked digitonin-solubilized His-P2X1 and His-P2X3 quantitatively to homo-trimers. Likewise, when analyzed by blue native PAGE, P2X receptors purified in digitonin or dodecyl-beta-D-maltoside migrated entirely as non-covalently linked homo-trimers, whereas the alpha2 beta gamma delta nicotinic acetylcholine receptor (used as a positive control) migrated as the expected pentamer. P2X monomers remained undetected soon after synthesis, indicating that trimerization occurred in the endoplasmic reticulum. The plasma membrane form of His-P2X1 was also identified as a homo-trimer. If n-octylglucoside was used for P2X receptor solubilization, homo-hexamers were observed, suggesting that trimers can aggregate to form larger complexes. We conclude that trimers represent an essential element of P2X receptor structure. blue native PAGE/cross-linking/P2X receptor/quaternary structure.
Abbracchio,
Purinoceptors: are there families of P2X and P2Y purinoceptors?
1994, Pubmed
Abbracchio,
Purinoceptors: are there families of P2X and P2Y purinoceptors?
1994,
Pubmed
Anand,
Neuronal nicotinic acetylcholine receptors expressed in Xenopus oocytes have a pentameric quaternary structure.
1991,
Pubmed
,
Xenbase
Betz,
Ligand-gated ion channels in the brain: the amino acid receptor superfamily.
1990,
Pubmed
Blount,
Membrane topology and multimeric structure of a mechanosensitive channel protein of Escherichia coli.
1996,
Pubmed
Bo,
Solubilization and molecular size determination of the P2x purinoceptor from rat vas deferens.
1992,
Pubmed
Boess,
Ultrastructure of the 5-hydroxytryptamine3 receptor.
1995,
Pubmed
Boulay,
Posttranslational oligomerization and cooperative acid activation of mixed influenza hemagglutinin trimers.
1988,
Pubmed
Buell,
An antagonist-insensitive P2X receptor expressed in epithelia and brain.
1996,
Pubmed
Buell,
P2X receptors: an emerging channel family.
1996,
Pubmed
Bültmann,
P2-purinoceptor antagonists: I. Blockade of P2-purinoceptor subtypes and ecto-nucleotidases by small aromatic isothiocyanato-sulphonates.
1996,
Pubmed
Burnashev,
Dimensions and ion selectivity of recombinant AMPA and kainate receptor channels and their dependence on Q/R site residues.
1996,
Pubmed
Burnstock,
Development and perspectives of the purinoceptor concept.
1996,
Pubmed
Burnstock,
Purinergic receptors: their role in nociception and primary afferent neurotransmission.
1996,
Pubmed
Cake,
Effect of pyridoxal phosphate on the DNA binding site of activated hepatic glucocorticoid receptor.
1978,
Pubmed
Chen,
A P2X purinoceptor expressed by a subset of sensory neurons.
1995,
Pubmed
,
Xenbase
Copeland,
Assembly of influenza hemagglutinin trimers and its role in intracellular transport.
1986,
Pubmed
Cruickshank,
Estimation of the pore size of the large-conductance mechanosensitive ion channel of Escherichia coli.
1997,
Pubmed
Dani,
Structure and function of glutamate and nicotinic acetylcholine receptors.
1995,
Pubmed
Doms,
Quaternary structure of influenza virus hemagglutinin after acid treatment.
1986,
Pubmed
Dwyer,
The permeability of the endplate channel to organic cations in frog muscle.
1980,
Pubmed
Evans,
Ionic permeability of, and divalent cation effects on, two ATP-gated cation channels (P2X receptors) expressed in mammalian cells.
1996,
Pubmed
Ferrer-Montiel,
Pentameric subunit stoichiometry of a neuronal glutamate receptor.
1996,
Pubmed
,
Xenbase
Firsov,
The heterotetrameric architecture of the epithelial sodium channel (ENaC).
1998,
Pubmed
,
Xenbase
Garcia-Guzman,
Characterization of recombinant human P2X4 receptor reveals pharmacological differences to the rat homologue.
1997,
Pubmed
,
Xenbase
Gloor,
A vector for the synthesis of cRNAs encoding Myc epitope-tagged proteins in Xenopus laevis oocytes.
1995,
Pubmed
,
Xenbase
Green,
Ion-channel assembly.
1995,
Pubmed
Hammond,
Quality control in the secretory pathway.
1995,
Pubmed
Hansen,
Structural motif and characteristics of the extracellular domain of P2X receptors.
1997,
Pubmed
Hollmann,
Cloned glutamate receptors.
1994,
Pubmed
Hucho,
The emerging three-dimensional structure of a receptor. The nicotinic acetylcholine receptor.
1996,
Pubmed
Johnston,
The multisubunit structure of synaptophysin. Relationship between disulfide bonding and homo-oligomerization.
1990,
Pubmed
Kim,
Disulfide-linked aggregation of thyroglobulin normally occurs during nascent protein folding.
1993,
Pubmed
Kim,
Molecular assembly of the extracellular domain of P2X2, an ATP-gated ion channel.
1997,
Pubmed
Kuner,
Structure of the NMDA receptor channel M2 segment inferred from the accessibility of substituted cysteines.
1996,
Pubmed
,
Xenbase
Lambrecht,
Design and pharmacology of selective P2-purinoceptor antagonists.
1996,
Pubmed
Lambrecht,
PPADS, a novel functionally selective antagonist of P2 purinoceptor-mediated responses.
1992,
Pubmed
Langosch,
Conserved quaternary structure of ligand-gated ion channels: the postsynaptic glycine receptor is a pentamer.
1988,
Pubmed
Laube,
Evidence for a tetrameric structure of recombinant NMDA receptors.
1998,
Pubmed
,
Xenbase
Lewis,
Coexpression of P2X2 and P2X3 receptor subunits can account for ATP-gated currents in sensory neurons.
1995,
Pubmed
Lingueglia,
Cloning of the amiloride-sensitive FMRFamide peptide-gated sodium channel.
1995,
Pubmed
,
Xenbase
Lutter,
The use of a new series of cleavable protein-crosslinkers on the Escherichia coli ribosome.
1974,
Pubmed
Mano,
A tetrameric subunit stoichiometry for a glutamate receptor-channel complex.
1998,
Pubmed
,
Xenbase
Montal,
Protein folds in channel structure.
1996,
Pubmed
Nayeem,
Quaternary structure of the native GABAA receptor determined by electron microscopic image analysis.
1994,
Pubmed
North,
Families of ion channels with two hydrophobic segments.
1996,
Pubmed
North,
P2X receptors: a third major class of ligand-gated ion channels.
1996,
Pubmed
Ortells,
Evolutionary history of the ligand-gated ion-channel superfamily of receptors.
1995,
Pubmed
Portoghese,
Bivalent ligands and the message-address concept in the design of selective opioid receptor antagonists.
1989,
Pubmed
Rassendren,
Identification of amino acid residues contributing to the pore of a P2X receptor.
1997,
Pubmed
Rassendren,
The permeabilizing ATP receptor, P2X7. Cloning and expression of a human cDNA.
1997,
Pubmed
Rossier,
Epithelial sodium channels.
1994,
Pubmed
Schägger,
Analysis of molecular masses and oligomeric states of protein complexes by blue native electrophoresis and isolation of membrane protein complexes by two-dimensional native electrophoresis.
1994,
Pubmed
Schägger,
Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form.
1991,
Pubmed
Schmalzing,
Isoform-specific interactions of Na,K-ATPase subunits are mediated via extracellular domains and carbohydrates.
1997,
Pubmed
,
Xenbase
Schmalzing,
Evidence for intracellular sodium pumps in permeabilized Xenopus laevis oocytes.
1989,
Pubmed
,
Xenbase
Schmalzing,
The adhesion molecule on glia (AMOG/beta 2) and alpha 1 subunits assemble to functional sodium pumps in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Schrempf,
A prokaryotic potassium ion channel with two predicted transmembrane segments from Streptomyces lividans.
1995,
Pubmed
Snyder,
Electrophysiological and biochemical evidence that DEG/ENaC cation channels are composed of nine subunits.
1998,
Pubmed
,
Xenbase
Surprenant,
The cytolytic P2Z receptor for extracellular ATP identified as a P2X receptor (P2X7).
1996,
Pubmed
Tatu,
Membrane glycoprotein folding, oligomerization and intracellular transport: effects of dithiothreitol in living cells.
1993,
Pubmed
Thomas,
Identification of synaptophysin as a hexameric channel protein of the synaptic vesicle membrane.
1988,
Pubmed
Thompson,
Selective radiolabeling of cell surface proteins to a high specific activity.
1987,
Pubmed
Unwin,
Acetylcholine receptor channel imaged in the open state.
1995,
Pubmed
Valera,
Characterization and chromosomal localization of a human P2X receptor from the urinary bladder.
1995,
Pubmed
,
Xenbase
Valera,
A new class of ligand-gated ion channel defined by P2x receptor for extracellular ATP.
1994,
Pubmed
,
Xenbase
Villarroel,
Dimensions of the narrow portion of a recombinant NMDA receptor channel.
1995,
Pubmed
,
Xenbase
Watty,
Fixation of allosteric states of the nicotinic acetylcholine receptor by chemical cross-linking.
1997,
Pubmed
,
Xenbase
Witzemann,
Primary structure and functional expression of the alpha-, beta-, gamma-, delta- and epsilon-subunits of the acetylcholine receptor from rat muscle.
1990,
Pubmed
,
Xenbase
Yang,
Determination of the subunit stoichiometry of an inwardly rectifying potassium channel.
1995,
Pubmed
,
Xenbase
Yeager,
Structure of gap junction intercellular channels.
1996,
Pubmed
