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Evidence for rapid metabolic turnover of hyaluronate synthetase in Swarm rat chondrosarcoma chondrocytes.
Bansal MK
,
Mason RM
.
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Synthesis of [3H]hyaluronate from [6-3H]glucosamine was investigated in cultures of Swarm rat chondrosarcoma chondrocytes treated with various concentrations (0.1 microM-0.1 mM) of cycloheximide for various times. Concentrations greater than 1 microM inhibited protein synthesis by greater than 90%. Hyaluronate synthesis was decreased, with a t1/2 for 50% inhibition of 80-120 min, depending on the concentration of cycloheximide present. Similar experiments using [1-3H]glucose as a precursor label gave similar results. Experiments using [6-3H]glucosamine as a precursor label and 0.18 mM-puromycin to inhibit protein synthesis inhibited hyaluronate synthesis (t1/2 = 82 min) with similar kinetics to cycloheximide-induced inhibition. Cultures incubated with 3.6 microM-cycloheximide for up to 9 h and supplemented with p-nitrophenyl beta-D-xyloside during the last 75 min of treatment showed increased synthesis of [3H,35S]chondroitin sulphate, demonstrating that UDP-hexose precursors for glycosaminoglycan synthesis are not rapidly depleted on blockage of protein synthesis. Rapid metabolic turnover of hyaluronate synthetase is the most likely cause for decreased hyaluronate synthesis in chondrocytes in which protein synthesis is inhibited.
ALLEN,
The effect of puromycin on rabbit reticulocyte ribosomes.
1962, Pubmed
ALLEN,
The effect of puromycin on rabbit reticulocyte ribosomes.
1962,
Pubmed
Caputo,
Effect of puromycin on cartilage proteoglycan structure and capacity to bind hyaluronic acid.
1984,
Pubmed
Kimata,
Nascent mucopolysaccharides attached to the Golgi membrane of chondrocytes.
1971,
Pubmed
Kimura,
Biosynthesis of proteoglycans and their assembly into aggregates in cultures of chondrocytes from the Swarm rat chondrosarcoma.
1979,
Pubmed
Kimura,
Identification of core protein, an intermediate in proteoglycan biosynthesis in cultured chondrocytes from the Swarm rat chondrosarcoma.
1981,
Pubmed
Kimura,
The effect of cycloheximide on synthesis of proteoglycans by cultured chondrocytes from the Swarm rat chondrosarcoma.
1981,
Pubmed
Kimura,
Studies on the biosynthesis of cartilage proteoglycan in a model system of cultured chondrocytes from the Swarm rat chondrosarcoma.
1984,
Pubmed
Kleine,
Hyaluronate-proteoglycan complex: evidence for separate biosynthesis mechanisms of the macromolecules.
1978,
Pubmed
Kubo,
Effect of puromycin on sugar transport in isolated rat adipocytes.
1985,
Pubmed
Lamberg,
Synthesis and degradation of hyaluronic acid in the cultured fibroblasts of Marfan's disease.
1973,
Pubmed
Mapleson,
Effect of cycloheximide and dexamethasone phosphate on hyaluronic acid synthesis and secretion in cultured human skin fibroblasts.
1981,
Pubmed
Maroudas,
Glycosaminoglycan turn-over in articular cartilage.
1975,
Pubmed
Martinez,
Sites of sulfatation in the chondrocytes of the articular cartilage of the rabbit. A study by quantitative radioautography of high resolution.
1977,
Pubmed
Mason,
Recent advances in the biochemistry of hyaluronic acid in cartilage.
1981,
Pubmed
Mason,
Absence of covalently linked core protein from newly synthesized hyaluronate.
1982,
Pubmed
Mason,
Biosynthesis of hyaluronic acid in cultures of chondrocytes from the Swarm rat chondrosarcoma.
1982,
Pubmed
Matalon,
The structure of acid mucopolysaccharides produced by Hurler fibroblasts in tissue culture.
1968,
Pubmed
Mikuni-Takagaki,
Hyaluronate-protein complex of rous sarcoma virus-transformed chick embryo fibroblasts.
1981,
Pubmed
Mitchell,
The effects of cycloheximide on the biosynthesis and secretion of proteoglycans by chondrocytes in culture.
1981,
Pubmed
NATHANS,
Amino acid transfer from aminoacyl-ribonucleic acids to protein on ribosomes of Escherichia coli.
1961,
Pubmed
Okayama,
The influence of p-nitrophenyl beta-d-xyloside on the synthesis of proteochondroitin sulfate by slices of embryonic chick cartilage.
1973,
Pubmed
Pestka,
The use of inhibitors in studies on protein synthesis.
1974,
Pubmed
Philipson,
Subcellular localization of hyaluronate synthetase in oligodendroglioma cells.
1984,
Pubmed
,
Xenbase
Pike,
Suppression of cytidylate biosynthesis by protein synthesis antagonists.
1980,
Pubmed
Prehm,
Synthesis of hyaluronate in differentiated teratocarcinoma cells. Characterization of the synthase.
1983,
Pubmed
,
Xenbase
Prehm,
Synthesis of hyaluronate in differentiated teratocarcinoma cells. Mechanism of chain growth.
1983,
Pubmed
Prehm,
Hyaluronate is synthesized at plasma membranes.
1984,
Pubmed
Robinson,
The effect of D-xylose, beta-D-xylosides and beta-D-galactosides on chondroitin sulphate biosynthesis in embryonic chicken cartilage.
1975,
Pubmed
Schwartz,
Stimulation of synthesis of free chondroitin sulfate chains by beta-D-xylosides in cultured cells.
1974,
Pubmed
Silbert,
Biosynthesis of chondroitin sulphate by a Golgi-apparatus-enriched preparation from cultures of mouse mastocytoma cells.
1980,
Pubmed
Smith,
Partial inhibition by cycloheximide of haluronate synthesis in cell culture.
1968,
Pubmed
Telser,
The biosynthesis of chondroitin-sulfate protein complex.
1965,
Pubmed
Thonar,
Biosynthesis of O-linked oligosaccharides on proteoglycans by chondrocytes from the swarm rat chondrosarcoma.
1983,
Pubmed
Volkin,
Suppression of the biosynthesis of guanosine triphosphate by protein synthesis inhibitors.
1980,
Pubmed
von Figura,
Differently labelled glucosamine-precursor pools for the biosynthesis of hyaluronate and heparan sulfate.
1973,
Pubmed
