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In vitro microtubule-nucleating activity of spindle pole bodies in fission yeast Schizosaccharomyces pombe: cell cycle-dependent activation in xenopus cell-free extracts.
Masuda H
,
Sevik M
,
Cande WZ
.
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The spindle pole body (SPB) is the equivalent of the centrosome in fission yeast. In vivo it nucleates microtubules (MTs) during mitosis, but, unlike animal centrosomes, does not act as a microtubule organizing center (MTOC) during interphase. We have studied the MT-nucleating activity of SPBs in vitro and have found that SPBs in permeabilized cells retain in vivo characteristics. SPBs in cells permeabilized during mitosis can nucleate MTs, and are recognized by two antibodies: anti-gamma-tubulin and MPM-2 which recognizes phosphoepitopes. SPBs in cells permeabilized during interphase cannot nucleate MTs and are only recognized by anti-gamma-tubulin. Interphase SPBs which cannot nucleate can be converted to a nucleation competent state by incubation in cytostatic factor (CSF)-arrested Xenopus egg extracts. After incubation, they are recognized by MPM-2, and can nucleate MTs. The conversion does not occur in Xenopus interphase extract, but occurs in Xenopus interphase extract driven into mitosis by preincubation with exogenous cyclin B. The conversion is ATP dependent and inhibited by protein kinase inhibitors and alkaline phosphatase. Purified, active, cdc2 kinase/cyclin B complex in itself is not effective for activation of MT nucleation, although some interphase SPBs are now stained with MPM-2. These results suggest that the ability of SPBs in vitro to nucleate MTs after exposure to CSF-arrested extracts is activated through a downstream pathway which is regulated by cdc2 kinase.
Alfa,
Distinct nuclear and spindle pole body population of cyclin-cdc2 in fission yeast.
1990, Pubmed
Alfa,
Distinct nuclear and spindle pole body population of cyclin-cdc2 in fission yeast.
1990,
Pubmed
Bailly,
p34cdc2 is located in both nucleus and cytoplasm; part is centrosomally associated at G2/M and enters vesicles at anaphase.
1989,
Pubmed
Belmont,
Real-time visualization of cell cycle-dependent changes in microtubule dynamics in cytoplasmic extracts.
1990,
Pubmed
,
Xenbase
Bré,
Effects of brain microtubule-associated proteins on microtubule dynamics and the nucleating activity of centrosomes.
1990,
Pubmed
Byers,
Behavior of spindles and spindle plaques in the cell cycle and conjugation of Saccharomyces cerevisiae.
1975,
Pubmed
Centonze,
Nucleation of microtubules from mitotic centrosomes is modulated by a phosphorylated epitope.
1990,
Pubmed
Davis,
Monoclonal antibodies to mitotic cells.
1983,
Pubmed
,
Xenbase
Engle,
Cell-cycle modulation of MPM-2-specific spindle pole body phosphorylation in Aspergillus nidulans.
1988,
Pubmed
Gorbsky,
Microtubule distribution in cultured cells and intact tissues: improved immunolabeling resolution through the use of reversible embedment cytochemistry.
1985,
Pubmed
Gotoh,
In vitro effects on microtubule dynamics of purified Xenopus M phase-activated MAP kinase.
1991,
Pubmed
,
Xenbase
Gould,
The pericentriolar material in Chinese hamster ovary cells nucleates microtubule formation.
1977,
Pubmed
Hagan,
The use of cell division cycle mutants to investigate the control of microtubule distribution in the fission yeast Schizosaccharomyces pombe.
1988,
Pubmed
Hirano,
A temperature-sensitive mutation of the Schizosaccharomyces pombe gene nuc2+ that encodes a nuclear scaffold-like protein blocks spindle elongation in mitotic anaphase.
1988,
Pubmed
Horio,
The fission yeast gamma-tubulin is essential for mitosis and is localized at microtubule organizing centers.
1991,
Pubmed
Kanbe,
The transition of cells of the fission yeast beta-tubulin mutant nda3-311 as seen by freeze-substitution electron microscopy. Requirement of functional tubulin for spindle pole body duplication.
1990,
Pubmed
Kuriyama,
Microtubule-nucleating activity of centrosomes in Chinese hamster ovary cells is independent of the centriole cycle but coupled to the mitotic cycle.
1981,
Pubmed
Leslie,
Recruitment: the ins and outs of spindle pole formation.
1990,
Pubmed
Leslie,
A component of the interphase cytoskeleton is cyclically recruited into spindle poles during mitosis.
1991,
Pubmed
Lohka,
Induction of nuclear envelope breakdown, chromosome condensation, and spindle formation in cell-free extracts.
1985,
Pubmed
,
Xenbase
Masuda,
In vitro reactivation of spindle elongation in fission yeast nuc2 mutant cells.
1990,
Pubmed
Mazia,
Centrosomes and mitotic poles.
1984,
Pubmed
McCully,
Mitosis in the fission yeast Schizosaccharomyces pombe: a comparative study with light and electron microscopy.
1971,
Pubmed
Mitchison,
Properties of the kinetochore in vitro. II. Microtubule capture and ATP-dependent translocation.
1985,
Pubmed
Murray,
Cyclin synthesis drives the early embryonic cell cycle.
1989,
Pubmed
,
Xenbase
Murray,
The role of cyclin synthesis and degradation in the control of maturation promoting factor activity.
1989,
Pubmed
,
Xenbase
Novak,
CO2 production after induction synchrony of the fission yeast Schizosaccharomyces pombe: the origin and nature of entrainment.
1990,
Pubmed
Nurse,
Universal control mechanism regulating onset of M-phase.
1990,
Pubmed
Oakley,
Identification of gamma-tubulin, a new member of the tubulin superfamily encoded by mipA gene of Aspergillus nidulans.
1989,
Pubmed
Oakley,
Gamma-tubulin is a component of the spindle pole body that is essential for microtubule function in Aspergillus nidulans.
1990,
Pubmed
Pfaller,
Assembly/disassembly of the nuclear envelope membrane: cell cycle-dependent binding of nuclear membrane vesicles to chromatin in vitro.
1991,
Pubmed
,
Xenbase
Riabowol,
The cdc2 kinase is a nuclear protein that is essential for mitosis in mammalian cells.
1989,
Pubmed
,
Xenbase
Rout,
Components of the yeast spindle and spindle pole body.
1990,
Pubmed
Rüegg,
Staurosporine, K-252 and UCN-01: potent but nonspecific inhibitors of protein kinases.
1989,
Pubmed
Sager,
A novel mitotic spindle pole component that originates from the cytoplasm during prophase.
1986,
Pubmed
Saxton,
Tubulin dynamics in cultured mammalian cells.
1984,
Pubmed
Snyder,
Initiation and growth of microtubules from mitotic centers in lysed mammalian cells.
1975,
Pubmed
Solomon,
Cyclin activation of p34cdc2.
1990,
Pubmed
,
Xenbase
Stearns,
Gamma-tubulin is a highly conserved component of the centrosome.
1991,
Pubmed
,
Xenbase
Tanaka,
Mitosis in the fission yeast Schizosaccharomyces pombe as revealed by freeze-substitution electron microscopy.
1986,
Pubmed
Uzawa,
The fission yeast cut1+ gene regulates spindle pole body duplication and has homology to the budding yeast ESP1 gene.
1990,
Pubmed
Vale,
Severing of stable microtubules by a mitotically activated protein in Xenopus egg extracts.
1991,
Pubmed
,
Xenbase
Vandre,
Phosphoproteins are components of mitotic microtubule organizing centers.
1984,
Pubmed
Verde,
Regulation of microtubule dynamics by cdc2 protein kinase in cell-free extracts of Xenopus eggs.
1990,
Pubmed
,
Xenbase
Verde,
Taxol-induced microtubule asters in mitotic extracts of Xenopus eggs: requirement for phosphorylated factors and cytoplasmic dynein.
1991,
Pubmed
,
Xenbase
Zeligs,
Mitosis in rat thyroid epithelial cells in vivo. II. Centrioles and pericentriolar material.
1979,
Pubmed
Zheng,
Gamma-tubulin is present in Drosophila melanogaster and Homo sapiens and is associated with the centrosome.
1991,
Pubmed
