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Probing α-3(10) transitions in a voltage-sensing S4 helix.
Kubota T
,
Lacroix JJ
,
Bezanilla F
,
Correa AM
.
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The S4 helix of voltage sensor domains (VSDs) transfers its gating charges across the membrane electrical field in response to changes of the membrane potential. Recent studies suggest that this process may occur via the helical conversion of the entire S4 between α and 310 conformations. Here, using LRET and FRET, we tested this hypothesis by measuring dynamic changes in the transmembrane length of S4 from engineered VSDs expressed in Xenopus oocytes. Our results suggest that the native S4 from the Ciona intestinalis voltage-sensitive phosphatase (Ci-VSP) does not exhibit extended and long-lived 310 conformations and remains mostly α-helical. Although the S4 of NavAb displays a fully extended 310 conformation in x-ray structures, its transplantation in the Ci-VSP VSD scaffold yielded similar results as the native Ci-VSP S4. Taken together, our study does not support the presence of long-lived extended α-to-310 helical conversions of the S4 in Ci-VSP associated with voltage activation.
Barondeau,
Mechanism and energetics of green fluorescent protein chromophore synthesis revealed by trapped intermediate structures.
2003, Pubmed
Barondeau,
Mechanism and energetics of green fluorescent protein chromophore synthesis revealed by trapped intermediate structures.
2003,
Pubmed
Bezanilla,
The voltage sensor in voltage-dependent ion channels.
2000,
Pubmed
Catterall,
Ion channel voltage sensors: structure, function, and pathophysiology.
2010,
Pubmed
Cha,
Structural implications of fluorescence quenching in the Shaker K+ channel.
1998,
Pubmed
Clayton,
Structure of the transmembrane regions of a bacterial cyclic nucleotide-regulated channel.
2008,
Pubmed
Doyle,
The structure of the potassium channel: molecular basis of K+ conduction and selectivity.
1998,
Pubmed
Gamal El-Din,
A gating charge interaction required for late slow inactivation of the bacterial sodium channel NavAb.
2013,
Pubmed
Giraldez,
Let's twist (the S4) again.
2014,
Pubmed
Henrion,
Tracking a complete voltage-sensor cycle with metal-ion bridges.
2012,
Pubmed
,
Xenbase
HODGKIN,
A quantitative description of membrane current and its application to conduction and excitation in nerve.
1952,
Pubmed
Hyde,
Nano-positioning system for structural analysis of functional homomeric proteins in multiple conformations.
2012,
Pubmed
,
Xenbase
Kohout,
Subunit organization and functional transitions in Ci-VSP.
2008,
Pubmed
Kuzmenkin,
Gating of the bacterial sodium channel, NaChBac: voltage-dependent charge movement and gating currents.
2004,
Pubmed
Lacroix,
Properties of deactivation gating currents in Shaker channels.
2011,
Pubmed
Lee,
Dimeric subunit stoichiometry of the human voltage-dependent proton channel Hv1.
2008,
Pubmed
Li,
Structural mechanism of voltage-dependent gating in an isolated voltage-sensing domain.
2014,
Pubmed
,
Xenbase
Long,
Atomic structure of a voltage-dependent K+ channel in a lipid membrane-like environment.
2007,
Pubmed
Murata,
Phosphoinositide phosphatase activity coupled to an intrinsic voltage sensor.
2005,
Pubmed
,
Xenbase
Nitz,
Structural origin of the high affinity of a chemically evolved lanthanide-binding peptide.
2004,
Pubmed
Noda,
Primary structure of Electrophorus electricus sodium channel deduced from cDNA sequence.
,
Pubmed
Payandeh,
Crystal structure of a voltage-gated sodium channel in two potentially inactivated states.
2012,
Pubmed
Payandeh,
The crystal structure of a voltage-gated sodium channel.
2011,
Pubmed
Peng,
Purification and structural study of the voltage-sensor domain of the human KCNQ1 potassium ion channel.
2014,
Pubmed
Pless,
Contributions of counter-charge in a potassium channel voltage-sensor domain.
2011,
Pubmed
,
Xenbase
Posson,
Small vertical movement of a K+ channel voltage sensor measured with luminescence energy transfer.
2005,
Pubmed
,
Xenbase
Posson,
Extent of voltage sensor movement during gating of shaker K+ channels.
2008,
Pubmed
,
Xenbase
Ramsey,
A voltage-gated proton-selective channel lacking the pore domain.
2006,
Pubmed
Sandtner,
In vivo measurement of intramolecular distances using genetically encoded reporters.
2007,
Pubmed
,
Xenbase
Sasaki,
A voltage sensor-domain protein is a voltage-gated proton channel.
2006,
Pubmed
Schwaiger,
3₁₀-helix conformation facilitates the transition of a voltage sensor S4 segment toward the down state.
2011,
Pubmed
Selvin,
Principles and biophysical applications of lanthanide-based probes.
2002,
Pubmed
Seoh,
Voltage-sensing residues in the S2 and S4 segments of the Shaker K+ channel.
1996,
Pubmed
,
Xenbase
Shaner,
Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein.
2004,
Pubmed
Shu,
Novel chromophores and buried charges control color in mFruits.
2006,
Pubmed
Stefani,
Cut-open oocyte voltage-clamp technique.
1998,
Pubmed
,
Xenbase
Tao,
A gating charge transfer center in voltage sensors.
2010,
Pubmed
,
Xenbase
Tombola,
The voltage-gated proton channel Hv1 has two pores, each controlled by one voltage sensor.
2008,
Pubmed
,
Xenbase
Vargas,
In search of a consensus model of the resting state of a voltage-sensing domain.
2011,
Pubmed
Vieira-Pires,
3(10) helices in channels and other membrane proteins.
2010,
Pubmed
Villalba-Galea,
S4-based voltage sensors have three major conformations.
2008,
Pubmed
Yarov-Yarovoy,
Structural basis for gating charge movement in the voltage sensor of a sodium channel.
2012,
Pubmed
