Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Physiol Sci
2014 Mar 01;642:141-50. doi: 10.1007/s12576-013-0303-6.
Show Gene links
Show Anatomy links
Electrostatic charge at position 552 affects the activation and permeation of FMRFamide-gated Na+ channels.
Kodani Y
,
Furukawa Y
.
???displayArticle.abstract???
The FMRFamide-gated Na(+) channel (FaNaC) is a unique peptide-gated sodium channel and a member of the epithelial sodium channel/degenerin family. Previous studies have shown that an aspartate residue (Asp(552)) in the second transmembrane domain is involved in activation of the FaNaC. To examine the significance of a negative charge at position 552, we used a cysteine-modification method. Macroscopic currents of a cysteine mutant (D552C) were potentiated or inhibited by use of positively or negatively charged sulfhydryl reagents ([2-(trimethylammonium)ethyl]methanethiosulfonate bromide, MTSET, and sodium (2-sulfonatoethyl)methanethiosulfonate, MTSES, respectively). Dose-response analysis showed that treatment with MTSET increased the potency of the FMRFamide in the FaNaC whereas treatment with MTSES reduced the maximum response. Negative charge at position 552 was necessary for the characteristic inward rectification of the FaNaC. These results suggest that negative electric charge at position 552 is important to the activation and permeation properties of the FaNaC.
Adams,
Protons activate brain Na+ channel 1 by inducing a conformational change that exposes a residue associated with neurodegeneration.
1998, Pubmed,
Xenbase
Adams,
Protons activate brain Na+ channel 1 by inducing a conformational change that exposes a residue associated with neurodegeneration.
1998,
Pubmed
,
Xenbase
Brown,
Gain-of-function mutations in the MEC-4 DEG/ENaC sensory mechanotransduction channel alter gating and drug blockade.
2007,
Pubmed
,
Xenbase
Canessa,
Epithelial sodium channel related to proteins involved in neurodegeneration.
1993,
Pubmed
,
Xenbase
Driscoll,
The mec-4 gene is a member of a family of Caenorhabditis elegans genes that can mutate to induce neuronal degeneration.
1991,
Pubmed
Furukawa,
Molecular cloning and functional characterization of the Aplysia FMRFamide-gated Na+ channel.
2006,
Pubmed
,
Xenbase
Golubovic,
A peptide-gated ion channel from the freshwater polyp Hydra.
2007,
Pubmed
Gonzales,
Pore architecture and ion sites in acid-sensing ion channels and P2X receptors.
2009,
Pubmed
Guerois,
Predicting changes in the stability of proteins and protein complexes: a study of more than 1000 mutations.
2002,
Pubmed
Huang,
Gene interactions affecting mechanosensory transduction in Caenorhabditis elegans.
1994,
Pubmed
Jasti,
Structure of acid-sensing ion channel 1 at 1.9 A resolution and low pH.
2007,
Pubmed
Karlin,
Substituted-cysteine accessibility method.
1998,
Pubmed
Kellenberger,
Epithelial sodium channel/degenerin family of ion channels: a variety of functions for a shared structure.
2002,
Pubmed
Kodani,
Position 552 in a FMRFamide-gated Na(+) channel affects the gating properties and the potency of FMRFamide.
2010,
Pubmed
,
Xenbase
Krissinel,
Inference of macromolecular assemblies from crystalline state.
2007,
Pubmed
Krivov,
Improved prediction of protein side-chain conformations with SCWRL4.
2009,
Pubmed
Li,
Interaction of the aromatics Tyr-72/Trp-288 in the interface of the extracellular and transmembrane domains is essential for proton gating of acid-sensing ion channels.
2009,
Pubmed
,
Xenbase
Lingueglia,
FMRFamide-gated sodium channel and ASIC channels: a new class of ionotropic receptors for FMRFamide and related peptides.
2006,
Pubmed
Passero,
Conformational changes associated with proton-dependent gating of ASIC1a.
2009,
Pubmed
,
Xenbase
Pau,
EBImage--an R package for image processing with applications to cellular phenotypes.
2010,
Pubmed
Paukert,
Identification of the Ca2+ blocking site of acid-sensing ion channel (ASIC) 1: implications for channel gating.
2004,
Pubmed
,
Xenbase
Pettersen,
UCSF Chimera--a visualization system for exploratory research and analysis.
2004,
Pubmed
Sali,
Comparative protein modelling by satisfaction of spatial restraints.
1993,
Pubmed
Schreibmayer,
Voltage clamping of Xenopus laevis oocytes utilizing agarose-cushion electrodes.
1994,
Pubmed
,
Xenbase
Schymkowitz,
Prediction of water and metal binding sites and their affinities by using the Fold-X force field.
2005,
Pubmed
Snyder,
Gating induces a conformational change in the outer vestibule of ENaC.
2000,
Pubmed
,
Xenbase
Teichman,
Aspartate-444 is essential for productive substrate interactions in a neuronal glutamate transporter.
2007,
Pubmed
,
Xenbase
Tolino,
Insights into the mechanism of pore opening of acid-sensing ion channel 1a.
2011,
Pubmed
,
Xenbase
Voss,
3V: cavity, channel and cleft volume calculator and extractor.
2010,
Pubmed
Waldmann,
The mammalian degenerin MDEG, an amiloride-sensitive cation channel activated by mutations causing neurodegeneration in Caenorhabditis elegans.
1996,
Pubmed
Waldmann,
Molecular cloning of a non-inactivating proton-gated Na+ channel specific for sensory neurons.
1997,
Pubmed
Yu,
A nonproton ligand sensor in the acid-sensing ion channel.
2010,
Pubmed
