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	Figure 1. The two different ASIC1a/2a heteromers can be distinguished via their apparent affinity for protons.(a) Representative current traces of homo- and heteromeric ASICs, which were activated for 10 s with solutions of decreasing pH and allowed to recover for 50 s at pH 7.4. (b) Concentration-response curves of normalized currents. Maximal current amplitudes were 5.0 ± 0.8 μA (ASIC1a), 16.1 ± 2.7 μA (ASIC1a/2a), 13.3 ± 3.4 μA (ASIC1a-2a-1a), 11.3 ± 3.3 μA (ASIC1a-2a-2a), and 1.9 ± 0.3 μA (ASIC2a), respectively. The EC50 values of ASIC1a-2a-1a and ASIC1a-2a-2a were significantly different (p < 0.001). There was also a slight significant difference in the Hill-coefficients (p < 0.05). Data are reported as mean ± SEM (n = 12–17 individual oocytes).
	Figure 2. The two different ASIC1a/2a heteromers can be distinguished via their steady-state desensitization curves.(a) Representative current traces of homo- and heteromeric ASICs, which were activated for 10 s with pH 4.0 (pH 6.0 for ASIC1a) and allowed to recover for 120 s in a bath solution with decreasing pH. (b) Concentration-response curves of normalized currents. Maximal current amplitudes were 9.0 ± 2.2 μA (ASIC1a), 22.9 ± 2.3 μA (ASIC1a/2a), 25.7 ± 2.1 μA (ASIC1a-2a-1a), 12.5 ± 1.7 μA (ASIC1a-2a-2a), and 2.8 ± 0.4 μA (ASIC2a), respectively. The curves of both concatamers differ in their IC50 values (p < 0.001) as well as in their Hill-coefficients (p < 0.001), which were similar to that of the dominant ASIC form in the constructs. Data are reported as mean ± SEM (n = 8–16 individual oocytes).
	Figure 3. Tachyphylaxis is absent in ASIC1a/2a heteromers.ASICs were repeatedly activated for 10 s with pH 4.0 and allowed to recover for 50 s at pH 7.4. Only homomeric ASIC1a showed tachyphylaxis. Maximal current amplitudes were 30.6 ± 3.6 μA (ASIC1a), 26.2 ± 3.6 μA (ASIC1a/2a), 20.3 ± 5.0 μA (ASIC1a-2a-1a), and 15.1 ± 4.0 μA (ASIC1a-2a-2a), respectively. Data are reported as mean ± SEM (n = 4–9 individual oocytes; measurements for ASIC1a are from one week).
	Figure 4. Both concatamers of ASIC1a/2a are potentiated by Zn2+.Concentration-response curves for normalized currents with and without Zn2+. The EC50 values of ASIC1a/2a (p = 0.045), ASIC1a-2a-1a () and ASIC1a-2a-2a (p < 0.01) were significantly left-shifted in the presence of 300 μm Zn2+. There was also a significant increase in the Hill-coefficient of ASIC1a-2a-1a (p < 0.001) and ASIC1a/2a (p < 0.01) but not of ASIC1a-2a-2a (p = 0.8). Maximal current amplitudes were 14.5 ± 3.1 μA (ASIC1a/2a), 20.3 ± 3.5 μA (ASIC1a/2a + Zn2+), 14.2 ± 3.1 μA (ASIC1a-2a-1a), 17.6 ± 3.4 μA (ASIC1a-2a-1a + Zn2+), 12.3 ± 1.8 μA (ASIC1a-2a-2a) and 11.9 ± 3.3 μA (ASIC1a-2a-2a + Zn2+), respectively. Data are reported as mean ± SEM (n = 10–13 individual oocytes).
	Figure 5. Inhibition by PcTx1 can distinguish between the two ASIC1a/2a heteromers.(a) Representative current traces showing the inhibition of different ASICs by PcTx1. ASICs were activated with pH 4.0 (pH 6.0 for homomeric ASIC1a) for 10 s and allowed to recover for 120 s in pH 6.95 (pH 7.4 for ASIC1a). 50 nM PcTx1 was applied in the conditioning solution before the second activation. (b) Comparison of the inhibition by PcTx1 of the different ASICs. The difference between ASIC1a-2a-1a and ASIC1a-2a-2a (p < 0.001) as well as the difference between ASIC1a-2a-1a and ASIC1a/2a (p < 0.001) were significant. There was no significant difference between ASIC1a/2a and ASIC1a-2a-2a. Maximal current amplitudes were 9.6 ± 1.9 μA (ASIC1a), 15.2 ± 1.7 μA (ASIC1a/2a), 12.9 ± 2.8 μA (ASIC1a-2a-1a), and 9.1 ± 1.4 μA (ASIC1a-2a-2a), respectively. Data are reported as mean ± SEM (n = 8–10).
	Figure 6. PcTx1 inhibits ASIC1a/2a heteromers in rat cortical neurons.(a) Representative current trace showing the inhibition of ASICs by PcTx1 at conditioning pH 6.95. ASICs were activated with pH 6.0 for 15 s and allowed to recover for 60 s in pH 6.95. 50 nM PcTx1 was applied in the conditioning solution before the third activation. (b) Quantitative analysis of six measurements like the one shown in (a). Symbols represent six individual neurons, bars represent mean and SD, respectively. Conditioning pH 6.95 desensitized homomeric ASIC1a and heteromeric ASIC1a/2b. Co-application of PcTx1 revealed the PcTx1-sensitivity of the remaining ASIC1a/2a heteromers in these neurons.
	Figure 7. Possible interactions of PcTx1 with subunit interfaces of the ASIC1a-2a-2a heteromer.The homology model of the heteromer with ASIC1a (blue) and ASIC2a (red) is shown in cartoon and PcTx1 (green) in solvent-accessible surface representation. Boxes illustrate the amino acids (stick and spheres representation) in the ASIC1a/2a subunit interfaces that interact with PcTx1, namely Gly214, Gly216, Glu236, Thr238, Glu241, and Glu352 in rASIC2a and Gly215, Gly217, Asp237, Thr239, Glu242, Asp349, and Glu353 in rASIC1a. Five interacting amino acids are completely conserved between cASIC1, rASIC1a and rASIC2a. Numbers of amino acids refer to rASIC1a and rASIC2a, respectively.

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