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Structure and function of the nontranscribed spacer regions of yeast rDNA.
Skryabin KG
,
Eldarov MA
,
Larionov VL
,
Bayev AA
,
Klootwijk J
,
de Regt VC
,
Veldman GM
,
Planta RJ
,
Georgiev OI
,
Hadjiolov AA
.
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The sequences of the nontranscribed spacers (NTS) of cloned ribosomal DNA (rDNA) units from both Saccharomyces cerevisiae and Saccharomyces carlsbergensis were determined. The NTS sequences of both species were found to be 93% homologous. The major disparities comprise different frequencies of reiteration of short tracts of six to sixteen basepairs. Most of these reiterations are found within the 1100 basepairs long NTS between the 3'-ends of 26S and 5S rRNA (NTS1). The NTS between the starts of 5S rRNA and 37S pre-rRNA (NTS2) comprises about 1250 basepairs. The first 800 basepairs of NTS NTS2 (adjacent to the 5S rRNA gene) are virtually identical in both strains whereas a variable region is present at about 250 basepairs upstream of the RNA polymerase A transcription start. In contrast to the situation in Drosophila and Xenopus no reiterations of the putative RNA polymerase A promoter are present within the yeast NTS. The strands of the yeast NTS reveal a remarkable bias of G and C-residues. Yeast rDNA was previously shown to contain a sequence capable of autonomous replication (ARS) (Szostak, J.W. and Wu, R (1979), Plasmid 2, 536-554). This ARS, which may correspond to a chromosomal origin of replication, was located on a fragment of 570 basepairs within NTS2.
Bayev,
The structure of the yeast ribosomal RNA genes. 2. The nucleotide sequence of the initiation site for ribosomal RNA transcription.
1980, Pubmed
Bayev,
The structure of the yeast ribosomal RNA genes. 2. The nucleotide sequence of the initiation site for ribosomal RNA transcription.
1980,
Pubmed
Bayev,
The structure of the yeast ribosomal RNA genes. 3. Precise mapping of the 18 S and 25 S rRNA genes and structure of the adjacent regions.
1981,
Pubmed
Bell,
Ribosomal RNA genes of Saccharomyces cerevisiae. I. Physical map of the repeating unit and location of the regions coding for 5 S, 5.8 S, 18 S, and 25 S ribosomal RNAs.
1977,
Pubmed
Bicknell,
Nucleic acid homologies among species of Saccharomyces.
1970,
Pubmed
Botchan,
A specific replication origin in the chromosomal rDNA of Lytechinus variegatus.
1982,
Pubmed
Broach,
Localization and sequence analysis of yeast origins of DNA replication.
1983,
Pubmed
Broach,
Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene.
1979,
Pubmed
Cech,
Replication of the extrachromosomal ribosomal RNA genes of Tetrahymena thermophilia.
1981,
Pubmed
Clarke,
Isolation of a yeast centromere and construction of functional small circular chromosomes.
1980,
Pubmed
Clark-Walker,
Hybridizable sequences between cytoplasmic ribosomal RNAs and 3 micron circular DNAs of Saccharomyces cerevisiae and Torulopsis glabrata.
1980,
Pubmed
Clewell,
Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event.
1970,
Pubmed
Coen,
Multiple Pol I initiation sequences in rDNA spacers of Drosophila melanogaster.
1982,
Pubmed
,
Xenbase
Coen,
Dynamics of concerted evolution of ribosomal DNA and histone gene families in the melanogaster species subgroup of Drosophila.
1982,
Pubmed
Cramer,
Denaturation mapping of the ribosomal DNA of Saccharomyces cerevisiae.
1980,
Pubmed
Cramer,
Isolation and characterization of DNA of Saccharomyces cerevisiae.
1972,
Pubmed
Georgiev,
The structure of the yeast ribosomal RNA genes. 4. Complete sequence of the 25 S rRNA gene from Saccharomyces cerevisae.
1981,
Pubmed
,
Xenbase
Hsiao,
High-frequency transformation of yeast by plasmids containing the cloned yeast ARG4 gene.
1979,
Pubmed
Humphreys,
A simple method for the preparation of large quantities of pure plasmid DNA.
1975,
Pubmed
Klein,
Intrachromosomal gene conversion in yeast.
1981,
Pubmed
Koch,
Sequencing of the 16S-23S spacer in a ribosomal RNA operon of Zea mays chloroplast DNA reveals two split tRNA genes.
1981,
Pubmed
Larionov,
3 micron DNA - an extrachromosomal ribosomal DNA in the yeast Saccharomyces cerevisiae.
1980,
Pubmed
Long,
Repeated genes in eukaryotes.
1980,
Pubmed
,
Xenbase
Lund,
Transfer RNA genes between 16S and 23S rRNA genes in rRNA transcription units of E. coli.
1976,
Pubmed
Maxam,
A new method for sequencing DNA.
1977,
Pubmed
Meyerink,
Topographical analysis of yeast ribosomal DNA by cleavage with restriction endonucleases. II. The physical map of EcoRI fragments.
1976,
Pubmed
Miller,
Transcription of the 'non-transcribed' spacer of Drosophila melanogaster rDNA.
1983,
Pubmed
Moss,
A transcriptional function for the repetitive ribosomal spacer in Xenopus laevis.
,
Pubmed
,
Xenbase
Moss,
The putative promoter of a Xenopus laevis ribosomal gene is reduplicated.
1979,
Pubmed
,
Xenbase
Nath,
Organization of the yeast ribosomal RNA gene cluster via cloning and restriction analysis.
1977,
Pubmed
Nellen,
The nucleotide sequences of the actin genes from Saccharomyces carlsbergensis and Saccharomyces cerevisiae are identical except for their introns.
1981,
Pubmed
Petes,
Yeast ribosomal DNA genes are located on chromosome XII.
1979,
Pubmed
Petes,
Characterization of two types of yeast ribosomal DNA genes.
1978,
Pubmed
Petes,
Unequal meiotic recombination within tandem arrays of yeast ribosomal DNA genes.
1980,
Pubmed
Simeone,
Sequence organization of the ribosomal spacer of D.melanogaster.
1982,
Pubmed
Smith,
Evolution of repeated DNA sequences by unequal crossover.
1976,
Pubmed
Swanson,
RNA polymerase I-dependent selective transcription of yeast ribosomal DNA. Identification of a new cellular ribosomal RNA precursor.
1983,
Pubmed
Szostak,
Unequal crossing over in the ribosomal DNA of Saccharomyces cerevisiae.
1980,
Pubmed
Szostak,
Insertion of a genetic marker into the ribosomal DNA of yeast.
1979,
Pubmed
Truett,
The replication of ribosomal DNA in the macronucleus of Tetrahymena.
1977,
Pubmed
Tschumper,
Sequence of a yeast DNA fragment containing a chromosomal replicator and the TRP1 gene.
1980,
Pubmed
Valenzuela,
Ribosomal RNA genes of Saccharomyces cerevisiae. II. Physical map and nucleotide sequence of the 5 S ribosomal RNA gene and adjacent intergenic regions.
1977,
Pubmed
Veldman,
The primary and secondary structure of yeast 26S rRNA.
1981,
Pubmed
Verbeet,
Molecular cloning of the rDNA of Saccharomyces rosei and comparison of its transcription initiation region with that of Saccharomyces carlsbergensis.
1983,
Pubmed
Zamb,
Analysis of the junction between ribosomal RNA genes and single-copy chromosomal sequences in the yeast Saccharomyces cerevisiae.
1982,
Pubmed
