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EMBO J
2001 Dec 17;2024:7041-51. doi: 10.1093/emboj/20.24.7041.
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Coupled and uncoupled proton movement by amino acid transport system N.
Chaudhry FA
,
Krizaj D
,
Larsson P
,
Reimer RJ
,
Wreden C
,
Storm-Mathisen J
,
Copenhagen D
,
Kavanaugh M
,
Edwards RH
.
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The system N transporter SN1 has been proposed to mediate the efflux of glutamine from cells required to sustain the urea cycle and the glutamine-glutamate cycle that regenerates glutamate and gamma-aminobutyric acid (GABA) for synaptic release. We now show that SN1 also mediates an ionic conductance activated by glutamine, and this conductance is selective for H(+). Although SN1 couples amino acid uptake to H(+) exchange, the glutamine-gated H(+) conductance is not stoichiometrically coupled to transport. Protons thus permeate SN1 both coupled to and uncoupled from amino acid flux, providing novel mechanisms to regulate the transfer of glutamine between cells.
Barnett,
Inhibition of Müller cell glutamine synthetase rapidly impairs the retinal response to light.
2000, Pubmed
Barnett,
Inhibition of Müller cell glutamine synthetase rapidly impairs the retinal response to light.
2000,
Pubmed
Chaudhry,
Molecular analysis of system N suggests novel physiological roles in nitrogen metabolism and synaptic transmission.
1999,
Pubmed
Chaudhry,
Glutamine uptake by neurons: interaction of protons with system a transporters.
2002,
Pubmed
Chaudhry,
Glutamate transporters in glial plasma membranes: highly differentiated localizations revealed by quantitative ultrastructural immunocytochemistry.
1995,
Pubmed
Conti,
Glutamate immunoreactivity in rat cerebral cortex is reversibly abolished by 6-diazo-5-oxo-L-norleucine (DON), an inhibitor of phosphate-activated glutaminase.
1994,
Pubmed
Fairman,
Arachidonic acid elicits a substrate-gated proton current associated with the glutamate transporter EAAT4.
1998,
Pubmed
,
Xenbase
Fei,
Primary structure, genomic organization, and functional and electrogenic characteristics of human system N 1, a Na+- and H+-coupled glutamine transporter.
2000,
Pubmed
,
Xenbase
Hamberger,
Extra- and intracellular amino acids in the hippocampus during development of hepatic encephalopathy.
1984,
Pubmed
Hamberger,
Glutamate as a CNS transmitter. I. Evaluation of glucose and glutamine as precursors for the synthesis of preferentially released glutamate.
1979,
Pubmed
Haüssinger,
Nitrogen metabolism in liver: structural and functional organization and physiological relevance.
1990,
Pubmed
Kanner,
Sodium-coupled neurotransmitter transport: structure, function and regulation.
1994,
Pubmed
Kilberg,
Characteristics of an amino acid transport system in rat liver for glutamine, asparagine, histidine, and closely related analogs.
1980,
Pubmed
Krizaj,
Compartmentalization of calcium extrusion mechanisms in the outer and inner segments of photoreceptors.
1998,
Pubmed
Laake,
Glutamine from glial cells is essential for the maintenance of the nerve terminal pool of glutamate: immunogold evidence from hippocampal slice cultures.
1995,
Pubmed
Liman,
Subunit stoichiometry of a mammalian K+ channel determined by construction of multimeric cDNAs.
1992,
Pubmed
,
Xenbase
McGale,
Studies of the inter-relationship between cerebrospinal fluid and plasma amino acid concentrations in normal individuals.
1977,
Pubmed
Nagaraja,
Glutamine transport in mouse cerebral astrocytes.
1996,
Pubmed
Patel,
Concentration of free amino acids in primary cultures of neurones and astrocytes.
1985,
Pubmed
Pouysségur,
Use of tritium-labeled precursors to select mutants.
1987,
Pubmed
Pow,
Glutamate in some retinal neurons is derived solely from glia.
1994,
Pubmed
Reimer,
Amino acid transport system A resembles system N in sequence but differs in mechanism.
2000,
Pubmed
Rothstein,
Localization of neuronal and glial glutamate transporters.
1994,
Pubmed
Rothstein,
Alteration of striatal glutamate release after glutamine synthetase inhibition.
1984,
Pubmed
Schousboe,
Phosphate activated glutaminase activity and glutamine uptake in primary cultures of astrocytes.
1979,
Pubmed
Seal,
Excitatory amino acid transporters: a family in flux.
1999,
Pubmed
Sonders,
Channels in transporters.
1996,
Pubmed
Storm-Mathisen,
Ultrastructural immunocytochemical observations on the localization, metabolism and transport of glutamate in normal and ischemic brain tissue.
1992,
Pubmed
Sugawara,
Cloning of an amino acid transporter with functional characteristics and tissue expression pattern identical to that of system A.
2000,
Pubmed
,
Xenbase
Taylor,
Transport of glutamine in Xenopus laevis oocytes: relationship with transport of other amino acids.
1989,
Pubmed
,
Xenbase
Thanki,
In vivo release from cerebral cortex of [14C]glutamate synthesized from [U-14C]glutamine.
1983,
Pubmed
Tzingounis,
Arachidonic acid activates a proton current in the rat glutamate transporter EAAT4.
1998,
Pubmed
,
Xenbase
Varoqui,
Cloning and functional identification of a neuronal glutamine transporter.
2000,
Pubmed
Wadiche,
Ion fluxes associated with excitatory amino acid transport.
1995,
Pubmed
,
Xenbase
Webb,
Direct measurement of intracellular pH changes in Xenopus eggs at fertilization and cleavage.
1981,
Pubmed
,
Xenbase
Yao,
A novel system A isoform mediating Na+/neutral amino acid cotransport.
2000,
Pubmed
,
Xenbase
