Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Biol Chem
2009 Aug 28;28435:23312-21. doi: 10.1074/jbc.M109.003202.
Show Gene links
Show Anatomy links
Acetylation of histone H3 at the nucleosome dyad alters DNA-histone binding.
Manohar M
,
Mooney AM
,
North JA
,
Nakkula RJ
,
Picking JW
,
Edon A
,
Fishel R
,
Poirier MG
,
Ottesen JJ
.
???displayArticle.abstract???
Histone post-translational modifications are essential for regulating and facilitating biological processes such as RNA transcription and DNA repair. Fifteen modifications are located in the DNA-histone dyad interface and include the acetylation of H3-K115 (H3-K115Ac) and H3-K122 (H3-K122Ac), but the functional consequences of these modifications are unknown. We have prepared semisynthetic histone H3 acetylated at Lys-115 and/or Lys-122 by expressed protein ligation and incorporated them into single nucleosomes. Competitive reconstitution analysis demonstrated that the acetylation of H3-K115 and H3-K122 reduces the free energy of histone octamer binding. Restriction enzyme kinetic analysis suggests that these histone modifications do not alter DNA accessibility near the sites of modification. However, acetylation of H3-K122 increases the rate of thermal repositioning. Remarkably, Lys --> Gln substitution mutations, which are used to mimic Lys acetylation, do not fully duplicate the effects of the H3-K115Ac or H3-K122Ac modifications. Our results are consistent with the conclusion that acetylation in the dyad interface reduces DNA-histone interaction(s), which may facilitate nucleosome repositioning and/or assembly/disassembly.
Adkins,
Chromatin disassembly mediated by the histone chaperone Asf1 is essential for transcriptional activation of the yeast PHO5 and PHO8 genes.
2004, Pubmed
Adkins,
Chromatin disassembly mediated by the histone chaperone Asf1 is essential for transcriptional activation of the yeast PHO5 and PHO8 genes.
2004,
Pubmed
Anderson,
Sequence and position-dependence of the equilibrium accessibility of nucleosomal DNA target sites.
2000,
Pubmed
Anderson,
Effects of histone acetylation on the equilibrium accessibility of nucleosomal DNA target sites.
2001,
Pubmed
Becker,
ATP-dependent nucleosome remodeling.
2002,
Pubmed
Cocklin,
Identification of methylation and acetylation sites on mouse histone H3 using matrix-assisted laser desorption/ionization time-of-flight and nanoelectrospray ionization tandem mass spectrometry.
2003,
Pubmed
Cosgrove,
Regulated nucleosome mobility and the histone code.
2004,
Pubmed
English,
Structural basis for the histone chaperone activity of Asf1.
2006,
Pubmed
,
Xenbase
Ferreira,
Histone modifications influence the action of Snf2 family remodelling enzymes by different mechanisms.
2007,
Pubmed
Fischle,
Histone and chromatin cross-talk.
2003,
Pubmed
Flaus,
Sin mutations alter inherent nucleosome mobility.
2004,
Pubmed
He,
Facile synthesis of site-specifically acetylated and methylated histone proteins: reagents for evaluation of the histone code hypothesis.
2003,
Pubmed
,
Xenbase
Hyland,
Insights into the role of histone H3 and histone H4 core modifiable residues in Saccharomyces cerevisiae.
2005,
Pubmed
Kornberg,
Chromatin structure; oligomers of the histones.
1974,
Pubmed
Kouzarides,
Chromatin modifications and their function.
2007,
Pubmed
Kurumizaka,
Sin mutations of histone H3: influence on nucleosome core structure and function.
1997,
Pubmed
,
Xenbase
Li,
Acetylation of p53 inhibits its ubiquitination by Mdm2.
2002,
Pubmed
Li,
Nucleosomes facilitate their own invasion.
2004,
Pubmed
,
Xenbase
Lowary,
New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning.
1998,
Pubmed
Luger,
Expression and purification of recombinant histones and nucleosome reconstitution.
1999,
Pubmed
Luger,
Crystal structure of the nucleosome core particle at 2.8 A resolution.
1997,
Pubmed
Maier,
ATP-dependent chromatosome remodeling.
2008,
Pubmed
McGinty,
Chemically ubiquitylated histone H2B stimulates hDot1L-mediated intranucleosomal methylation.
2008,
Pubmed
,
Xenbase
Mersfelder,
The tale beyond the tail: histone core domain modifications and the regulation of chromatin structure.
2006,
Pubmed
Muir,
Semisynthesis of proteins by expressed protein ligation.
2003,
Pubmed
Muthurajan,
Crystal structures of histone Sin mutant nucleosomes reveal altered protein-DNA interactions.
2004,
Pubmed
Ottesen,
An amalgamation of solid phase peptide synthesis and ribosomal peptide synthesis.
2008,
Pubmed
Park,
Histone chaperones in nucleosome eviction and histone exchange.
2008,
Pubmed
Poirier,
Spontaneous access to DNA target sites in folded chromatin fibers.
2008,
Pubmed
Polach,
Mechanism of protein access to specific DNA sequences in chromatin: a dynamic equilibrium model for gene regulation.
1995,
Pubmed
Schwabish,
Asf1 mediates histone eviction and deposition during elongation by RNA polymerase II.
2006,
Pubmed
Segal,
A genomic code for nucleosome positioning.
2006,
Pubmed
Shogren-Knaak,
Histone H4-K16 acetylation controls chromatin structure and protein interactions.
2006,
Pubmed
Shogren-Knaak,
Creating designer histones by native chemical ligation.
2004,
Pubmed
,
Xenbase
Shrader,
Artificial nucleosome positioning sequences.
1989,
Pubmed
Simon,
The site-specific installation of methyl-lysine analogs into recombinant histones.
2007,
Pubmed
,
Xenbase
Strahl,
The language of covalent histone modifications.
2000,
Pubmed
Taverna,
How chromatin-binding modules interpret histone modifications: lessons from professional pocket pickers.
2007,
Pubmed
Thåström,
Nucleosomal locations of dominant DNA sequence motifs for histone-DNA interactions and nucleosome positioning.
2004,
Pubmed
Thåström,
Measurement of histone-DNA interaction free energy in nucleosomes.
2004,
Pubmed
Ura,
Histone acetylation: influence on transcription, nucleosome mobility and positioning, and linker histone-dependent transcriptional repression.
1997,
Pubmed
,
Xenbase
Wang,
Acetylation mimics within individual core histone tail domains indicate distinct roles in regulating the stability of higher-order chromatin structure.
2008,
Pubmed
,
Xenbase
Wang,
Chromatin remodeling and cancer, Part I: Covalent histone modifications.
2007,
Pubmed
Widlund,
DNA sequence-dependent contributions of core histone tails to nucleosome stability: differential effects of acetylation and proteolytic tail removal.
2000,
Pubmed
Yuan,
Genome-scale identification of nucleosome positions in S. cerevisiae.
2005,
Pubmed
Zhang,
Identification of novel histone post-translational modifications by peptide mass fingerprinting.
2003,
Pubmed
Zhang,
Identification of acetylation and methylation sites of histone H3 from chicken erythrocytes by high-accuracy matrix-assisted laser desorption ionization-time-of-flight, matrix-assisted laser desorption ionization-postsource decay, and nanoelectrospray ionization tandem mass spectrometry.
2002,
Pubmed
