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The dorsal-ventral axis in frog embryos is specified during the first cell cycle, when the cortex rotates relative to the cytoplasmic core along parallel microtubules associated with the core. Cytoplasmic transfer experiments suggest that dorsal determinants are transported 90 degrees from the vegetal pole to the dorsal equator, even though the cortex rotates only 30 degrees. Here we show that, during rotation, small endogenous organelles are rapidly propelled along the subcortical microtubules toward the future dorsal side and that fluorescent carboxylated beads injected into the vegetal pole are transported at least 60 degrees toward the equator. We also show that deuterium oxide, which broadens the zone of dorsalization even though it reduces the extent of rotation and is known to randomize the microtubules, also randomizes the direction of organelle transport. Moreover, beta-catenin, a component of the Wnt signaling pathway that possesses dorsalizing activity in Xenopus, colocalizes with subcortical microtubules at the dorsal side of the egg at the end of rotation. We propose that cortical rotation functions to align subcortical microtubules, which then mediate the transport of dorsal determinants toward their plus ends on one side of the egg.
Adams,
Rapid transport of foreign particles microinjected into crab axons.
, Pubmed
Adams,
Rapid transport of foreign particles microinjected into crab axons.
,
Pubmed
Cui,
Xwnt-8b: a maternally expressed Xenopus Wnt gene with a potential role in establishing the dorsoventral axis.
1995,
Pubmed
,
Xenbase
DeMarais,
The armadillo homologs beta-catenin and plakoglobin are differentially expressed during early development of Xenopus laevis.
1992,
Pubmed
,
Xenbase
Du,
Identification of distinct classes and functional domains of Wnts through expression of wild-type and chimeric proteins in Xenopus embryos.
1995,
Pubmed
,
Xenbase
Elinson,
A transient array of parallel microtubules in frog eggs: potential tracks for a cytoplasmic rotation that specifies the dorso-ventral axis.
1988,
Pubmed
,
Xenbase
Elinson,
Specifying the dorsoanterior axis in frogs: 70 years since Spemann and Mangold.
1995,
Pubmed
,
Xenbase
Fagotto,
Binding to cadherins antagonizes the signaling activity of beta-catenin during axis formation in Xenopus.
1996,
Pubmed
,
Xenbase
Fujisue,
Occurrence of dorsal axis-inducing activity around the vegetal pole of an uncleaved Xenopus egg and displacement to the equatorial region by cortical rotation.
1993,
Pubmed
,
Xenbase
Gerhart,
Cortical rotation of the Xenopus egg: consequences for the anteroposterior pattern of embryonic dorsal development.
1989,
Pubmed
,
Xenbase
Greenspan,
Nile red: a selective fluorescent stain for intracellular lipid droplets.
1985,
Pubmed
Guger,
beta-Catenin has Wnt-like activity and mimics the Nieuwkoop signaling center in Xenopus dorsal-ventral patterning.
1995,
Pubmed
,
Xenbase
Heasman,
Overexpression of cadherins and underexpression of beta-catenin inhibit dorsal mesoderm induction in early Xenopus embryos.
1994,
Pubmed
,
Xenbase
Holowacz,
Cortical cytoplasm, which induces dorsal axis formation in Xenopus, is inactivated by UV irradiation of the oocyte.
1993,
Pubmed
,
Xenbase
Houliston,
Evidence for the involvement of microtubules, ER, and kinesin in the cortical rotation of fertilized frog eggs.
1991,
Pubmed
,
Xenbase
Kelly,
Induction of a secondary embryonic axis in zebrafish occurs following the overexpression of beta-catenin.
1995,
Pubmed
,
Xenbase
Larabell,
Establishment of the dorso-ventral axis in Xenopus embryos is presaged by early asymmetries in beta-catenin that are modulated by the Wnt signaling pathway.
1997,
Pubmed
,
Xenbase
Larabell,
Confocal microscopy analysis of living Xenopus eggs and the mechanism of cortical rotation.
1996,
Pubmed
,
Xenbase
Moon,
Dissecting Wnt signalling pathways and Wnt-sensitive developmental processes through transient misexpression analyses in embryos of Xenopus laevis.
1993,
Pubmed
,
Xenbase
Munemitsu,
Regulation of intracellular beta-catenin levels by the adenomatous polyposis coli (APC) tumor-suppressor protein.
1995,
Pubmed
Papkoff,
Wnt-1 regulates free pools of catenins and stabilizes APC-catenin complexes.
1996,
Pubmed
Rubinfeld,
Association of the APC gene product with beta-catenin.
1993,
Pubmed
Sakai,
The vegetal determinants required for the Spemann organizer move equatorially during the first cell cycle.
1996,
Pubmed
,
Xenbase
Savage,
Dynamics of germ plasm localization and its inhibition by ultraviolet irradiation in early cleavage Xenopus embryos.
1993,
Pubmed
,
Xenbase
Scharf,
Hyperdorsoanterior embryos from Xenopus eggs treated with D2O.
1989,
Pubmed
,
Xenbase
Schneider,
Beta-catenin translocation into nuclei demarcates the dorsalizing centers in frog and fish embryos.
1996,
Pubmed
,
Xenbase
Su,
Association of the APC tumor suppressor protein with catenins.
1993,
Pubmed
Terasaki,
Localization of endoplasmic reticulum in living and glutaraldehyde-fixed cells with fluorescent dyes.
1984,
Pubmed
Terasaki,
Transport of cytoskeletal elements in the squid giant axon.
1995,
Pubmed
Vincent,
Kinematics of gray crescent formation in Xenopus eggs: the displacement of subcortical cytoplasm relative to the egg surface.
1986,
Pubmed
,
Xenbase
Yost,
The axis-inducing activity, stability, and subcellular distribution of beta-catenin is regulated in Xenopus embryos by glycogen synthase kinase 3.
1996,
Pubmed
,
Xenbase
