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Mol Cell Biol
1999 Dec 01;1912:8412-21. doi: 10.1128/MCB.19.12.8412.
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Nuclear retention elements of U3 small nucleolar RNA.
Speckmann W
,
Narayanan A
,
Terns R
,
Terns MP
.
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The processing and methylation of precursor rRNA is mediated by the box C/D small nucleolar RNAs (snoRNAs). These snoRNAs differ from most cellular RNAs in that they are not exported to the cytoplasm. Instead, these RNAs are actively retained in the nucleus where they assemble with proteins into mature small nucleolar ribonucleoprotein particles and are targeted to their intranuclear site of action, the nucleolus. In this study, we have identified the cis-acting sequences responsible for the nuclear retention of U3 box C/D snoRNA by analyzing the nucleocytoplasmic distributions of an extensive panel of U3 RNA variants after injection of the RNAs into Xenopus oocyte nuclei. Our data indicate the importance of two conserved sequence motifs in retaining U3 RNA in the nucleus. The first motif is comprised of the conserved box C' and box D sequences that characterize the box C/D family. The second motif contains conserved box sequences B and C. Either motif is sufficient for nuclear retention, but disruption of both motifs leads to mislocalization of the RNAs to the cytoplasm. Variant RNAs that are not retained also lack 5' cap hypermethylation and fail to associate with fibrillarin. Furthermore, our results indicate that nuclear retention of U3 RNA does not simply reflect its nucleolar localization. A fragment of U3 containing the box B/C motif is not localized to nucleoli but retained in coiled bodies. Thus, nuclear retention and nucleolar localization are distinct processes with differing sequence requirements.
Andrade,
Human autoantibody to a novel protein of the nuclear coiled body: immunological characterization and cDNA cloning of p80-coilin.
1991, Pubmed
Andrade,
Human autoantibody to a novel protein of the nuclear coiled body: immunological characterization and cDNA cloning of p80-coilin.
1991,
Pubmed
Bachellerie,
Antisense snoRNAs: a family of nucleolar RNAs with long complementarities to rRNA.
1995,
Pubmed
Balakin,
The RNA world of the nucleolus: two major families of small RNAs defined by different box elements with related functions.
1996,
Pubmed
Baserga,
An intact Box C sequence in the U3 snRNA is required for binding of fibrillarin, the protein common to the major family of nucleolar snRNPs.
1991,
Pubmed
Bauer,
In vitro assembly of coiled bodies in Xenopus egg extract.
1994,
Pubmed
,
Xenbase
Beltrame,
Mutational analysis of an essential binding site for the U3 snoRNA in the 5' external transcribed spacer of yeast pre-rRNA.
1994,
Pubmed
Beltrame,
Base pairing between U3 and the pre-ribosomal RNA is required for 18S rRNA synthesis.
1995,
Pubmed
Birkenmeier,
A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes.
1978,
Pubmed
,
Xenbase
Boelens,
Nuclear retention of RNA as a mechanism for localization.
1995,
Pubmed
,
Xenbase
Bringmann,
Purification of snRNPs U1, U2, U4, U5 and U6 with 2,2,7-trimethylguanosine-specific antibody and definition of their constituent proteins reacting with anti-Sm and anti-(U1)RNP antisera.
1983,
Pubmed
Cheng,
Diverse effects of the guanine nucleotide exchange factor RCC1 on RNA transport.
1995,
Pubmed
Dunbar,
Mpp10p, a U3 small nucleolar ribonucleoprotein component required for pre-18S rRNA processing in yeast.
1997,
Pubmed
Enright,
5'ETS rRNA processing facilitated by four small RNAs: U14, E3, U17, and U3.
1996,
Pubmed
Epstein,
Multiple states of U3 RNA in Novikoff hepatoma nucleoli.
1984,
Pubmed
Gall,
Is the sphere organelle/coiled body a universal nuclear component?
1995,
Pubmed
,
Xenbase
Ganot,
The family of box ACA small nucleolar RNAs is defined by an evolutionarily conserved secondary structure and ubiquitous sequence elements essential for RNA accumulation.
1997,
Pubmed
Gao,
Human genes encoding U3 snRNA associate with coiled bodies in interphase cells and are clustered on chromosome 17p11.2 in a complex inverted repeat structure.
1997,
Pubmed
Gerbi,
Small nucleolar RNA.
1995,
Pubmed
Ginisty,
Nucleolin functions in the first step of ribosomal RNA processing.
1998,
Pubmed
,
Xenbase
Glibetic,
Regulation of U3 snRNA expression during myoblast differentiation.
1992,
Pubmed
Greenwood,
Molecular characterization of U3 small nucleolar RNA from the early diverging protist, Euglena gracilis.
1996,
Pubmed
Hamm,
Monomethylated cap structures facilitate RNA export from the nucleus.
1990,
Pubmed
,
Xenbase
Hartshorne,
A common core structure for U3 small nucleolar RNAs.
1994,
Pubmed
Hughes,
The yeast homologue of U3 snRNA.
1987,
Pubmed
,
Xenbase
Hughes,
Depletion of U3 small nucleolar RNA inhibits cleavage in the 5' external transcribed spacer of yeast pre-ribosomal RNA and impairs formation of 18S ribosomal RNA.
1991,
Pubmed
Hughes,
Functional base-pairing interaction between highly conserved elements of U3 small nucleolar RNA and the small ribosomal subunit RNA.
1996,
Pubmed
Izaurralde,
Nuclear localization of a cap-binding protein complex.
1995,
Pubmed
,
Xenbase
Izaurralde,
A cap binding protein that may mediate nuclear export of RNA polymerase II-transcribed RNAs.
1992,
Pubmed
,
Xenbase
Jansen,
A U3 snoRNP protein with homology to splicing factor PRP4 and G beta domains is required for ribosomal RNA processing.
1993,
Pubmed
,
Xenbase
Jarmolowski,
Nuclear export of different classes of RNA is mediated by specific factors.
1994,
Pubmed
,
Xenbase
Jeppesen,
Nucleotide sequence determination and secondary structure of Xenopus U3 snRNA.
1988,
Pubmed
,
Xenbase
Jiménez-García,
Nucleologenesis: U3 snRNA-containing prenucleolar bodies move to sites of active pre-rRNA transcription after mitosis.
1994,
Pubmed
Kass,
The U3 small nucleolar ribonucleoprotein functions in the first step of preribosomal RNA processing.
1990,
Pubmed
Kiss,
Molecular analysis of a U3 RNA gene locus in tomato: transcription signals, the coding region, expression in transgenic tobacco plants and tandemly repeated pseudogenes.
1990,
Pubmed
Kiss,
Alteration of the RNA polymerase specificity of U3 snRNA genes during evolution and in vitro.
1991,
Pubmed
Kiss-László,
Sequence and structural elements of methylation guide snoRNAs essential for site-specific ribose methylation of pre-rRNA.
1998,
Pubmed
Lafontaine,
Nop58p is a common component of the box C+D snoRNPs that is required for snoRNA stability.
1999,
Pubmed
Lange,
Nucleolar localization elements in U8 snoRNA differ from sequences required for rRNA processing.
1998,
Pubmed
,
Xenbase
Lange,
Conserved boxes C and D are essential nucleolar localization elements of U14 and U8 snoRNAs.
1998,
Pubmed
,
Xenbase
Lange,
Nucleolar localization elements of Xenopus laevis U3 small nucleolar RNA.
1998,
Pubmed
,
Xenbase
Lapeyre,
Molecular cloning of Xenopus fibrillarin, a conserved U3 small nuclear ribonucleoprotein recognized by antisera from humans with autoimmune disease.
1990,
Pubmed
,
Xenbase
Leader,
Characterisation and expression of a maize U3 snRNA gene.
1994,
Pubmed
Lee,
Imp3p and Imp4p, two specific components of the U3 small nucleolar ribonucleoprotein that are essential for pre-18S rRNA processing.
1999,
Pubmed
Lübben,
Isolation of U3 snoRNP from CHO cells: a novel 55 kDa protein binds to the central part of U3 snoRNA.
1993,
Pubmed
Matera,
Of coiled bodies, gems, and salmon.
1998,
Pubmed
Mattaj,
Cap trimethylation of U snRNA is cytoplasmic and dependent on U snRNP protein binding.
1986,
Pubmed
,
Xenbase
Maxwell,
The small nucleolar RNAs.
1995,
Pubmed
Mazan,
Structure of the differentially expressed mouse U3A gene.
1992,
Pubmed
Méreau,
An in vivo and in vitro structure-function analysis of the Saccharomyces cerevisiae U3A snoRNP: protein-RNA contacts and base-pair interaction with the pre-ribosomal RNA.
1997,
Pubmed
Mougey,
A U3 small nuclear ribonucleoprotein-requiring processing event in the 5' external transcribed spacer of Xenopus precursor rRNA.
1993,
Pubmed
,
Xenbase
Munns,
Antibody-nucleic acid complexes. Immunospecific retention of globin messenger ribonucleic acid with antibodies specific for 7-methylguanosine.
1982,
Pubmed
Narayanan,
Nucleolar localization signals of box H/ACA small nucleolar RNAs.
1999,
Pubmed
,
Xenbase
Narayanan,
Role of the box C/D motif in localization of small nucleolar RNAs to coiled bodies and nucleoli.
1999,
Pubmed
,
Xenbase
Orum,
Sequence and proposed secondary structure of the Tetrahymena thermophila U3-snRNA.
1993,
Pubmed
Parker,
Structural analysis of the human U3 ribonucleoprotein particle reveal a conserved sequence available for base pairing with pre-rRNA.
1987,
Pubmed
Peculis,
Sequence and structural elements critical for U8 snRNP function in Xenopus oocytes are evolutionarily conserved.
1994,
Pubmed
,
Xenbase
Pluk,
cDNA cloning and characterization of the human U3 small nucleolar ribonucleoprotein complex-associated 55-kilodalton protein.
1998,
Pubmed
Porter,
The sequence of U3 from Schizosaccharomyces pombe suggests structural divergence of this snRNA between metazoans and unicellular eukaryotes.
1988,
Pubmed
Reimer,
Monoclonal autoantibody from a (New Zealand black x New Zealand white)F1 mouse and some human scleroderma sera target an Mr 34,000 nucleolar protein of the U3 RNP particle.
1987,
Pubmed
Samarsky,
The snoRNA box C/D motif directs nucleolar targeting and also couples snoRNA synthesis and localization.
1998,
Pubmed
Samarsky,
Functional mapping of the U3 small nucleolar RNA from the yeast Saccharomyces cerevisiae.
1998,
Pubmed
Savino,
In vivo disruption of Xenopus U3 snRNA affects ribosomal RNA processing.
1990,
Pubmed
,
Xenbase
Schimmang,
A yeast nucleolar protein related to mammalian fibrillarin is associated with small nucleolar RNA and is essential for viability.
1989,
Pubmed
Sharma,
Base pairing between U3 small nucleolar RNA and the 5' end of 18S rRNA is required for pre-rRNA processing.
1999,
Pubmed
Shaw,
Localization and processing from a polycistronic precursor of novel snoRNAs in maize.
1998,
Pubmed
Sienna,
Altered subcellular distribution of U3 snRNA in response to serum in mouse fibroblasts.
1996,
Pubmed
Singh,
Gamma-monomethyl phosphate: a cap structure in spliceosomal U6 small nuclear RNA.
1989,
Pubmed
Smith,
Sno storm in the nucleolus: new roles for myriad small RNPs.
1997,
Pubmed
Suh,
Human U3 small nucleolar RNA genes are localized to the nucleoplasm.
1987,
Pubmed
Terns,
Retention and 5' cap trimethylation of U3 snRNA in the nucleus.
1994,
Pubmed
,
Xenbase
Terns,
Multiple cis-acting signals for export of pre-U1 snRNA from the nucleus.
1993,
Pubmed
,
Xenbase
Terns,
A common maturation pathway for small nucleolar RNAs.
1995,
Pubmed
,
Xenbase
Terns,
Nuclear transport of RNAs in microinjected Xenopus oocytes.
1998,
Pubmed
,
Xenbase
Tollervey,
The small nucleolar RNP protein NOP1 (fibrillarin) is required for pre-rRNA processing in yeast.
1991,
Pubmed
Tollervey,
Function and synthesis of small nucleolar RNAs.
1997,
Pubmed
Tuma,
Identification and characterization of a sphere organelle protein.
1993,
Pubmed
,
Xenbase
Tyc,
U3, U8 and U13 comprise a new class of mammalian snRNPs localized in the cell nucleolus.
1989,
Pubmed
Wiederkehr,
Synthetic lethal interactions with conditional poly(A) polymerase alleles identify LCP5, a gene involved in 18S rRNA maturation.
1998,
Pubmed
Wu,
Nop5p is a small nucleolar ribonucleoprotein component required for pre-18 S rRNA processing in yeast.
1998,
Pubmed
