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Proc Natl Acad Sci U S A
2010 Nov 02;10744:18862-7. doi: 10.1073/pnas.1010354107.
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Stoichiometry of the KCNQ1 - KCNE1 ion channel complex.
Nakajo K
,
Ulbrich MH
,
Kubo Y
,
Isacoff EY
.
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The KCNQ1 voltage-gated potassium channel and its auxiliary subunit KCNE1 play a crucial role in the regulation of the heartbeat. The stoichiometry of KCNQ1 and KCNE1 complex has been debated, with some results suggesting that the four KCNQ1 subunits that form the channel associate with two KCNE1 subunits (a 42 stoichiometry), while others have suggested that the stoichiometry may not be fixed. We applied a single molecule fluorescence bleaching method to count subunits in many individual complexes and found that the stoichiometry of the KCNQ1 - KCNE1 complex is flexible, with up to four KCNE1 subunits associating with the four KCNQ1 subunits of the channel (a 44 stoichiometry). The proportion of the various stoichiometries was found to depend on the relative expression densities of KCNQ1 and KCNE1. Strikingly, both the voltage-dependence and kinetics of gating were found to depend on the relative densities of KCNQ1 and KCNE1, suggesting the heart rhythm may be regulated by the relative expression of the auxiliary subunit and the resulting stoichiometry of the channel complex.
Angelo,
KCNE5 induces time- and voltage-dependent modulation of the KCNQ1 current.
2002, Pubmed,
Xenbase
Angelo,
KCNE5 induces time- and voltage-dependent modulation of the KCNQ1 current.
2002,
Pubmed
,
Xenbase
Barhanin,
K(V)LQT1 and lsK (minK) proteins associate to form the I(Ks) cardiac potassium current.
1996,
Pubmed
,
Xenbase
Chandrasekhar,
KCNE1 subunits require co-assembly with K+ channels for efficient trafficking and cell surface expression.
2006,
Pubmed
Chen,
Charybdotoxin binding in the I(Ks) pore demonstrates two MinK subunits in each channel complex.
2003,
Pubmed
,
Xenbase
Chung,
Location of KCNE1 relative to KCNQ1 in the I(KS) potassium channel by disulfide cross-linking of substituted cysteines.
2009,
Pubmed
Doyle,
The structure of the potassium channel: molecular basis of K+ conduction and selectivity.
1998,
Pubmed
Grunnet,
KCNE4 is an inhibitory subunit to the KCNQ1 channel.
2002,
Pubmed
,
Xenbase
Jiang,
Crystal structure and mechanism of a calcium-gated potassium channel.
2002,
Pubmed
Jiang,
X-ray structure of a voltage-dependent K+ channel.
2003,
Pubmed
Jiang,
Dynamic partnership between KCNQ1 and KCNE1 and influence on cardiac IKs current amplitude by KCNE2.
2009,
Pubmed
Kang,
Structure of KCNE1 and implications for how it modulates the KCNQ1 potassium channel.
2008,
Pubmed
Kang,
Functional delivery of a membrane protein into oocyte membranes using bicelles.
2010,
Pubmed
,
Xenbase
Kohout,
Subunit organization and functional transitions in Ci-VSP.
2008,
Pubmed
Krumerman,
An LQT mutant minK alters KvLQT1 trafficking.
2004,
Pubmed
Long,
Atomic structure of a voltage-dependent K+ channel in a lipid membrane-like environment.
2007,
Pubmed
Long,
Voltage sensor of Kv1.2: structural basis of electromechanical coupling.
2005,
Pubmed
Long,
Crystal structure of a mammalian voltage-dependent Shaker family K+ channel.
2005,
Pubmed
Manderfield,
KCNE4 can co-associate with the I(Ks) (KCNQ1-KCNE1) channel complex.
2008,
Pubmed
Melman,
KCNE1 binds to the KCNQ1 pore to regulate potassium channel activity.
2004,
Pubmed
Morin,
A derivatized scorpion toxin reveals the functional output of heteromeric KCNQ1-KCNE K+ channel complexes.
2007,
Pubmed
,
Xenbase
Morin,
Counting membrane-embedded KCNE beta-subunits in functioning K+ channel complexes.
2008,
Pubmed
,
Xenbase
Morokuma,
Modulation of potassium channel function confers a hyperproliferative invasive phenotype on embryonic stem cells.
2008,
Pubmed
,
Xenbase
Morokuma,
KCNQ1 and KCNE1 K+ channel components are involved in early left-right patterning in Xenopus laevis embryos.
2008,
Pubmed
,
Xenbase
Nakajo,
KCNE1 and KCNE3 stabilize and/or slow voltage sensing S4 segment of KCNQ1 channel.
2007,
Pubmed
,
Xenbase
Neyroud,
A novel mutation in the potassium channel gene KVLQT1 causes the Jervell and Lange-Nielsen cardioauditory syndrome.
1997,
Pubmed
Osteen,
The cardiac IKs channel, complex indeed.
2010,
Pubmed
,
Xenbase
Panaghie,
The role of S4 charges in voltage-dependent and voltage-independent KCNQ1 potassium channel complexes.
2007,
Pubmed
,
Xenbase
Poulsen,
The KCNE1 beta-subunit exerts a transient effect on the KCNQ1 K+ channel.
2007,
Pubmed
,
Xenbase
Rocheleau,
KCNE peptides differently affect voltage sensor equilibrium and equilibration rates in KCNQ1 K+ channels.
2008,
Pubmed
,
Xenbase
Romey,
Molecular mechanism and functional significance of the MinK control of the KvLQT1 channel activity.
1997,
Pubmed
Sanguinetti,
Coassembly of K(V)LQT1 and minK (IsK) proteins to form cardiac I(Ks) potassium channel.
1996,
Pubmed
,
Xenbase
Schroeder,
A constitutively open potassium channel formed by KCNQ1 and KCNE3.
2000,
Pubmed
,
Xenbase
Shamgar,
KCNE1 constrains the voltage sensor of Kv7.1 K+ channels.
2008,
Pubmed
,
Xenbase
Smith,
Structural models for the KCNQ1 voltage-gated potassium channel.
2007,
Pubmed
Sobolevsky,
X-ray structure, symmetry and mechanism of an AMPA-subtype glutamate receptor.
2009,
Pubmed
Takumi,
Cloning of a membrane protein that induces a slow voltage-gated potassium current.
1988,
Pubmed
,
Xenbase
Tinel,
KCNE2 confers background current characteristics to the cardiac KCNQ1 potassium channel.
2000,
Pubmed
,
Xenbase
Tombola,
The voltage-gated proton channel Hv1 has two pores, each controlled by one voltage sensor.
2008,
Pubmed
,
Xenbase
Toyoda,
Modulation of functional properties of KCNQ1 channel by association of KCNE1 and KCNE2.
2006,
Pubmed
Ulbrich,
Rules of engagement for NMDA receptor subunits.
2008,
Pubmed
,
Xenbase
Ulbrich,
Subunit counting in membrane-bound proteins.
2007,
Pubmed
,
Xenbase
Wang,
MinK-KvLQT1 fusion proteins, evidence for multiple stoichiometries of the assembled IsK channel.
1998,
Pubmed
Wang,
Subunit composition of minK potassium channels.
1995,
Pubmed
,
Xenbase
Xu,
KCNQ1 and KCNE1 in the IKs channel complex make state-dependent contacts in their extracellular domains.
2008,
Pubmed
,
Xenbase
Yu,
Structural and molecular basis of the assembly of the TRPP2/PKD1 complex.
2009,
Pubmed
,
Xenbase
