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Globin mRNAs of Xenopus borealis and Xenopus tropicalis have been cloned and sequenced. The nucleotide and derived amino acid sequences were compared with each other and with already available data from Xenopus laevis. This analysis rendered clear evidence that the common ancestor of X. laevis and X. borealis, but not of X. tropicalis, had lost one amino acid of the beta-globins prior to a genome duplication event that preceded the segregation of the former two species. Replacement-site substitutions were used to calculate a rough time scale of genome duplication and species segregation. The results suggest an ancient separation between the X. laevis and the X. tropicalis groups occurring approximately 110-120 million years ago. Analysis of the amino acid chains demonstrated various alterations. However, some functional domains, like heme-binding sites and alpha 1 beta 2 contact sites, were subject to a high degree of conservation, indicating the existence of functional constraints on them also in the genus Xenopus.
Andres,
Comparative analysis of the cDNA sequences derived from the larval and the adult alpha 1-globin mRNAs of Xenopus laevis.
1984, Pubmed,
Xenbase
Andres,
Comparative analysis of the cDNA sequences derived from the larval and the adult alpha 1-globin mRNAs of Xenopus laevis.
1984,
Pubmed
,
Xenbase
Aviv,
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
1972,
Pubmed
Birnboim,
A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
1979,
Pubmed
Bisbee,
Albumin phylogeny for clawed frogs (Xenopus).
1977,
Pubmed
,
Xenbase
Bürki,
Evolution of globin expression in the genus Xenopus (Anura: Pipidae).
1985,
Pubmed
,
Xenbase
Efstratiadis,
The structure and evolution of the human beta-globin gene family.
1980,
Pubmed
FANELLI,
Studies on the structure of hemoglobin. I. Physicochemical properties of human globin.
1958,
Pubmed
Fermi,
Three-dimensional fourier synthesis of human deoxyhaemoglobin at 2-5 A resolution: refinement of the atomic model.
1975,
Pubmed
Goodman,
The phylogeny of human globin genes investigated by the maximum parsimony method.
1974,
Pubmed
Goodman,
Darwinian evolution in the genealogy of haemoglobin.
1975,
Pubmed
Hosbach,
The Xenopus laevis globin gene family: chromosomal arrangement and gene structure.
1983,
Pubmed
,
Xenbase
Jeffreys,
Linkage of adult alpha- and beta-globin genes in X. laevis and gene duplication by tetraploidization.
1980,
Pubmed
,
Xenbase
Kay,
Complete nucleotide sequence of a cloned cDNA derived from the major adult alpha-globin mRNA of X. laevis.
1983,
Pubmed
,
Xenbase
Kimura,
Preponderance of synonymous changes as evidence for the neutral theory of molecular evolution.
1977,
Pubmed
Knöchel,
Cloning of cDNA sequences derived from poly(A)+ nuclear RNA ofXenopus laevis at different developmental stages: Evidence for stage specific regulation.
1981,
Pubmed
,
Xenbase
Knöchel,
Molecular cloning and sequencing of mRNAs coding for minor adult globin polypeptides of Xenopus laevis.
1983,
Pubmed
,
Xenbase
Knöchel,
No evidence for 'stress' alpha-globin genes in chicken.
1982,
Pubmed
Kobel,
Two transitions of haemoglobin expression in Xenopus: from embryonic to larval and from larval to adult.
1983,
Pubmed
,
Xenbase
Lee,
Superoxide dismutase: an evolutionary puzzle.
1985,
Pubmed
Lewin,
Molecular clocks scrutinized.
1985,
Pubmed
Marotta,
Human beta-globin messenger RNA. III. Nucleotide sequences derived from complementary DNA.
1977,
Pubmed
Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed
May,
Isolation and characterisation of the Xenopus laevis albumin genes: loss of 74K albumin gene sequences by library amplification.
1982,
Pubmed
,
Xenbase
Meyerhof,
The primary structure of the larval beta 1-globin gene of Xenopus laevis and its flanking regions.
1984,
Pubmed
,
Xenbase
Meyerhof,
Sequence analysis of the larval beta II-globin gene of Xenopus laevis.
1986,
Pubmed
,
Xenbase
Patient,
The complete nucleotide sequence of the major adult beta globin gene of Xenopus laevis.
1983,
Pubmed
,
Xenbase
Perler,
The evolution of genes: the chicken preproinsulin gene.
1980,
Pubmed
Richards,
Molecular cloning and sequence analysis of adult chicken betal globin cDNA.
1979,
Pubmed
Richardson,
Partial sequence analysis of Xenopus alpha- and beta-globin mRNA as determined from recombinant DNA plasmids.
1980,
Pubmed
,
Xenbase
Stalder,
Conserved sequences and cell-specific DNase I hypersensitive sites upstream from the co-ordinately expressed alpha I- and alpha II-globin genes of Xenopus laevis.
1986,
Pubmed
,
Xenbase
Takeshita,
Cloning and sequence analysis of a cDNA for the alpha-globin mRNA of carp, Cyprinus carpio.
1984,
Pubmed
Thiébaud,
DNA content in the genus Xenopus.
1977,
Pubmed
,
Xenbase
Tymowska,
The karyotype of the hexaploid species Xenopus ruwenzoriensis Fischberg and Kobel (Anura: Pipidae).
1980,
Pubmed
,
Xenbase
Tymowska,
A comparison of the karyotype, constitutive heterochromatin, and nucleolar organizer regions of the new tetraploid species Xenopus epitropicalis Fischberg and Picard with those of Xenopus tropicalis Gray (Anura, Pipidae).
1982,
Pubmed
,
Xenbase
Tymowska,
Karyotype analysis of Xenopus tropicalis Gray, Pipidae.
1973,
Pubmed
,
Xenbase
Wahli,
Isolation of two closely related vitellogenin genes, including their flanking regions, from a Xenopus laevis gene library.
1980,
Pubmed
,
Xenbase
Westley,
Xenopus laevis serum albumins are encoded in two closely related genes.
1981,
Pubmed
,
Xenbase
Williams,
The nucleotide sequence of the major beta-globin mRNA from Xenopus laevis.
1980,
Pubmed
,
Xenbase
Wilson,
Biochemical evolution.
1977,
Pubmed
Wilson,
Nucleotide sequence of the coding portion of human alpha globin messenger RNA.
1980,
Pubmed
Wilson,
Insertion of synthetic copies of human globin genes into bacterial plasmids.
1978,
Pubmed
Wu,
Evidence for higher rates of nucleotide substitution in rodents than in man.
1985,
Pubmed
Zain,
Nucleotide sequence analysis of the leader segments in a cloned copy of adenovirus 2 fiber mRNA.
1979,
Pubmed
