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DNA sequence-directed nucleosome reconstitution on 5S RNA genes of Xenopus laevis.
Gottesfeld JM
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Nucleosomes were reconstituted in vitro with several singly end-labeled restriction fragments derived from a cloned somatic-type 5S RNA gene of Xenopus laevis and purified nucleosome core particles from Xenopus cultured cells or chicken erythrocytes. Nucleosome locations were determined by digestion of the reconstitutes with exonuclease III and DNase I and were the same for all fragments investigated, extending from 20 base pairs (bp) within the 5S gene to 80 bp beyond the 3' end of the gene. Both core particles and crude nuclear extracts gave equivalent results, suggesting that no factors other than the core histones are responsible for recognition of DNA sequence during reconstitution. The histone octamer and the 5S gene-specific transcription factor TFIIIA both bind to the same region and face of 5S DNA, and nucleosome reconstitution on the 5S gene excluded binding of TFIIIA. The helical repeat of somatic-type 5S DNA in solution was measured by the band shift method and was 10.5 to 10.6 bp per turn over the region of the TFIIIA-binding site. The difference in helical repeat between DNA in solution and on the surface of the nucleosome (10.0-bp spacing between DNase I cutting sites) may explain the linking number paradox.
Böck,
Positioning of nucleosomes in satellite I-containing chromatin of rat liver.
1984, Pubmed
Böck,
Positioning of nucleosomes in satellite I-containing chromatin of rat liver.
1984,
Pubmed
Bogenhagen,
Stable transcription complexes of Xenopus 5S RNA genes: a means to maintain the differentiated state.
1982,
Pubmed
,
Xenbase
Chao,
DNA sequence directs placement of histone cores on restriction fragments during nucleosome formation.
1979,
Pubmed
Crick,
Linking numbers and nucleosomes.
1976,
Pubmed
Dickerson,
Base sequence and helix structure variation in B and A DNA.
1983,
Pubmed
Elgin,
DNAase I-hypersensitive sites of chromatin.
1981,
Pubmed
Emerson,
Interaction of specific nuclear factors with the nuclease-hypersensitive region of the chicken adult beta-globin gene: nature of the binding domain.
1985,
Pubmed
Engelke,
Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
1980,
Pubmed
,
Xenbase
Gottesfeld,
Nonrandom alignment of nucleosomes on 5S RNA genes of X. laevis.
1980,
Pubmed
,
Xenbase
Gottesfeld,
Assembly of transcriptionally active 5S RNA gene chromatin in vitro.
1982,
Pubmed
,
Xenbase
Klug,
The helical periodicity of DNA on the nucleosome.
1981,
Pubmed
Klug,
A low resolution structure for the histone core of the nucleosome.
1980,
Pubmed
Lutter,
Precise location of DNase I cutting sites in the nucleosome core determined by high resolution gel electrophoresis.
1979,
Pubmed
Lutter,
Kinetic analysis of deoxyribonuclease I cleavages in the nucleosome core: evidence for a DNA superhelix.
1978,
Pubmed
Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed
McCall,
The crystal structure of d(GGATGGGAG): an essential part of the binding site for transcription factor IIIA.
,
Pubmed
Mengeritsky,
Nucleotide sequence-directed mapping of the nucleosomes.
1983,
Pubmed
Miller,
Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes.
1985,
Pubmed
,
Xenbase
Peck,
Sequence dependence of the helical repeat of DNA in solution.
1981,
Pubmed
Pelham,
A specific transcription factor that can bind either the 5S RNA gene or 5S RNA.
1980,
Pubmed
,
Xenbase
Peterson,
Characterization of two xenopus somatic 5S DNAs and one minor oocyte-specific 5S DNA.
1980,
Pubmed
,
Xenbase
Prunell,
Relation of nucleosomes to DNA sequences.
1978,
Pubmed
Prunell,
Periodicity of deoxyribonuclease I digestion of chromatin.
1979,
Pubmed
Ramsay,
A 145-base pair DNA sequence that positions itself precisely and asymmetrically on the nucleosome core.
1984,
Pubmed
Reynolds,
5S rRNA gene transcription factor IIIA alters the helical configuration of DNA.
1983,
Pubmed
,
Xenbase
Rhodes,
An underlying repeat in some transcriptional control sequences corresponding to half a double helical turn of DNA.
1986,
Pubmed
,
Xenbase
Rhodes,
Structural analysis of a triple complex between the histone octamer, a Xenopus gene for 5S RNA and transcription factor IIIA.
1985,
Pubmed
,
Xenbase
Rhodes,
Helical periodicity of DNA determined by enzyme digestion.
1980,
Pubmed
Richmond,
Structure of the nucleosome core particle at 7 A resolution.
,
Pubmed
Sakonju,
Contact points between a positive transcription factor and the Xenopus 5S RNA gene.
1982,
Pubmed
,
Xenbase
Sakonju,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region.
1980,
Pubmed
,
Xenbase
Saragosti,
Absence of nucleosomes in a fraction of SV40 chromatin between the origin of replication and the region coding for the late leader RNA.
1980,
Pubmed
Simpson,
Mapping DNAase l-susceptible sites in nucleosomes labeled at the 5' ends.
1976,
Pubmed
Simpson,
Structural features of a phased nucleosome core particle.
1983,
Pubmed
Simpson,
Chromatin reconstituted from tandemly repeated cloned DNA fragments and core histones: a model system for study of higher order structure.
1985,
Pubmed
Sollner-Webb,
Pancreatic DNAase cleavage sites in nuclei.
1977,
Pubmed
Wang,
The path of DNA in the nucleosome.
1982,
Pubmed
Young,
Regular arrangement of nucleosomes on 5S rRNA genes in Xenopus laevis.
1983,
Pubmed
,
Xenbase
Zhurkin,
Specific alignment of nucleosomes on DNA correlates with periodic distribution of purine-pyrimidine and pyrimidine-purine dimers.
1983,
Pubmed
