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Lamins are karyoskeletal proteins associated with the nuclear envelope which can be divided into two groups, i.e. the type A lamins of near neutral pI and the more acidic lamins, including mammalian lamin B. We have isolated cDNA clones encoding a representative of the type B subfamily from Xenopus laevis, and have deduced its amino acid sequence from the coding portion of the approximately 2.9 kb mRNA. The polypeptide (mol. wt 66,433) is identified as a typical lamin by its homology to Xenopus human type A lamins, but detailed sequence comparison shows that LI is less related to Xenopus lamin A than the latter is to human lamin A. The conformation predicted for LI conforms to the general model of lamins and intermediate filament proteins and is characterized by an extended central alpha-helical coiled coil domain, flanked by non-alpha-helical domains, i.e. a relatively short N-terminal head and a long C-terminal tail. As in lamins A and C, the head of lamin LI is positively charged and the tail presents a similar C-terminal pentapeptide, a putative nuclear accumulation signal, a very negatively charged region and a number of short regions that are highly homologous in all lamins. However, LI differs from the type A lamins by the absence of the oligo-histidine stretch and a di-proline motif in the tail region and by a significantly lower number of identical amino acid positions.(ABSTRACT TRUNCATED AT 250 WORDS)
Aaronson,
Isolation of nuclear pore complexes in association with a lamina.
1975, Pubmed
Aaronson,
Isolation of nuclear pore complexes in association with a lamina.
1975,
Pubmed
Achtstaetter,
Separation of cytokeratin polypeptides by gel electrophoretic and chromatographic techniques and their identification by immunoblotting.
1986,
Pubmed
Aebi,
The nuclear lamina is a meshwork of intermediate-type filaments.
,
Pubmed
,
Xenbase
Benavente,
Change of karyoskeleton during spermatogenesis of Xenopus: expression of lamin LIV, a nuclear lamina protein specific for the male germ line.
1985,
Pubmed
,
Xenbase
Benavente,
Cell type-specific expression of nuclear lamina proteins during development of Xenopus laevis.
1985,
Pubmed
,
Xenbase
Burke,
A cell free system to study reassembly of the nuclear envelope at the end of mitosis.
1986,
Pubmed
Burke,
A monoclonal antibody which recognises each of the nuclear lamin polypeptides in mammalian cells.
1983,
Pubmed
Dingwall,
Nucleoplasmin cDNA sequence reveals polyglutamic acid tracts and a cluster of sequences homologous to putative nuclear localization signals.
1987,
Pubmed
,
Xenbase
Fisher,
cDNA sequencing of nuclear lamins A and C reveals primary and secondary structural homology to intermediate filament proteins.
1986,
Pubmed
Franke,
Biochemical and immunological identification of cytokeratin proteins present in hepatocytes of mammalian liver tissue.
1981,
Pubmed
Geisler,
The amino acid sequence of chicken muscle desmin provides a common structural model for intermediate filament proteins.
1982,
Pubmed
Gerace,
Organization and modulation of nuclear lamina structure.
1984,
Pubmed
Gerace,
Immunocytochemical localization of the major polypeptides of the nuclear pore complex-lamina fraction. Interphase and mitotic distribution.
1978,
Pubmed
Gerace,
The nuclear envelope lamina is reversibly depolymerized during mitosis.
1980,
Pubmed
Hatzfeld,
Cytokeratin domains involved in heterotypic complex formation determined by in-vitro binding assays.
1987,
Pubmed
Henikoff,
Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.
1984,
Pubmed
Jorcano,
Cell type-specific expression of bovine keratin genes as demonstrated by the use of complementary DNA clones.
1984,
Pubmed
Kalderon,
A short amino acid sequence able to specify nuclear location.
1984,
Pubmed
Kleinschmidt,
Molecular characterization of a karyophilic, histone-binding protein: cDNA cloning, amino acid sequence and expression of nuclear protein N1/N2 of Xenopus laevis.
1986,
Pubmed
,
Xenbase
Krohne,
Cell type-specific differences in protein composition of nuclear pore complex-lamina structures in oocytes and erythrocytes of Xenopus laevis.
1981,
Pubmed
,
Xenbase
Krohne,
The major polypeptides of the nuclear pore complex.
1978,
Pubmed
,
Xenbase
Krohne,
Localization of a nuclear envelope-associated protein by indirect immunofluorescence microscopy using antibodies against a major polypeptide from rat liver fractions enriched in nuclear envelope-associated material.
1978,
Pubmed
Krohne,
The nuclear lamins. A multigene family of proteins in evolution and differentiation.
1986,
Pubmed
,
Xenbase
Krohne,
A monoclonal antibody against nuclear lamina proteins reveals cell type-specificity in Xenopus laevis.
1984,
Pubmed
,
Xenbase
Lam,
Electrophoretic analysis of three major nuclear envelope polypeptides. Topological relationship and sequence homology.
1979,
Pubmed
Lebel,
Lamin B from rat liver nuclei exists both as a lamina protein and as an intrinsic membrane protein.
1984,
Pubmed
Lebel,
Lamins A, B and C share an epitope with the common domain of intermediate filament proteins.
1987,
Pubmed
Lehner,
Biogenesis of the nuclear lamina: in vivo synthesis and processing of nuclear protein precursors.
1986,
Pubmed
Lehner,
The nuclear lamin protein family in higher vertebrates. Identification of quantitatively minor lamin proteins by monoclonal antibodies.
1986,
Pubmed
Maul,
The major 67 000 molecular weight protein of the clam oocyte nuclear envelope is lamin-like.
1984,
Pubmed
Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed
McKeon,
Autoimmune response directed against conserved determinants of nuclear envelope proteins in a patient with linear scleroderma.
1983,
Pubmed
McKeon,
Homologies in both primary and secondary structure between nuclear envelope and intermediate filament proteins.
,
Pubmed
Osborn,
Cytoplasmic intermediate filament proteins and the nuclear lamins A, B and C share the IFA epitope.
1987,
Pubmed
Quinlan,
Heteropolymer filaments of vimentin and desmin in vascular smooth muscle tissue and cultured baby hamster kidney cells demonstrated by chemical crosslinking.
1982,
Pubmed
Quinlan,
Characterization of dimer subunits of intermediate filament proteins.
1986,
Pubmed
Rebagliati,
Identification and cloning of localized maternal RNAs from Xenopus eggs.
1985,
Pubmed
,
Xenbase
Ricciardi,
Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation.
1979,
Pubmed
Richardson,
Nuclear location signals in polyoma virus large-T.
1986,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Scheer,
Experimental disintegration of the nuclear envelope. Evidence for pore-connecting fibrils.
1976,
Pubmed
,
Xenbase
Shelton,
Oligomeric structure of the major nuclear envelope protein lamin B.
1982,
Pubmed
Smith,
Identification, developmental regulation, and response to heat shock of two antigenically related forms of a major nuclear envelope protein in Drosophila embryos: application of an improved method for affinity purification of antibodies using polypeptides immobilized on nitrocellulose blots.
1984,
Pubmed
Steinert,
The molecular biology of intermediate filaments.
1985,
Pubmed
Stick,
Changes in the nuclear lamina composition during early development of Xenopus laevis.
1985,
Pubmed
,
Xenbase
Vieira,
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
1982,
Pubmed
Wickner,
Multiple mechanisms of protein insertion into and across membranes.
1985,
Pubmed
Wolin,
A new lamin in Xenopus somatic tissues displays strong homology to human lamin A.
1987,
Pubmed
,
Xenbase
