Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
RNA-dependent cytoplasmic anchoring of a transcription factor subunit during Xenopus development.
Brzostowski J
,
Robinson C
,
Orford R
,
Elgar S
,
Scarlett G
,
Peterkin T
,
Malartre M
,
Kneale G
,
Wormington M
,
Guille M
.
???displayArticle.abstract???
The CCAAT box transcription factor (CBTF) is a multimeric transcription factor that activates expression of the haematopoietic regulatory factor, GATA-2. The 122 kDa subunit of this complex, CBTF(122), is cytoplasmic in fertilized Xenopus eggs and subsequently translocates to the nucleus prior to activation of zygotic GATA-2 transcription at gastrulation. Here we present data suggesting both a role for CBTF(122) prior to its nuclear translocation and the mechanism that retains it in the cytoplasm before the midblastula transition (MBT). CBTF(122) and its variant CBTF(98) are associated with translationally quiescent mRNP complexes. We show that CBTF(122) RNA binding activity is both necessary and sufficient for its cytoplasmic retention during early development. The introduction of an additional nuclear localization signal to CBTF(122) is insufficient to overcome this retention, suggesting that RNA binding acts as a cytoplasmic anchor for CBTF(122). Destruction of endogenous RNA by microinjection of RNase promotes premature nuclear translocation of CBTF(122). Thus, the nuclear translocation of CBTF(122) at the MBT is likely to be coupled to the degradation of maternal mRNA that occurs at that stage.
Bass,
Binding properties of newly identified Xenopus proteins containing dsRNA-binding motifs.
1994,
Pubmed
,
Xenbase
Beg,
I kappa B interacts with the nuclear localization sequences of the subunits of NF-kappa B: a mechanism for cytoplasmic retention.
1992,
Pubmed
Brewer,
Nuclear translocation of a maternal CCAAT factor at the start of gastrulation activates Xenopus GATA-2 transcription.
1995,
Pubmed
,
Xenbase
Burd,
Conserved structures and diversity of functions of RNA-binding proteins.
1994,
Pubmed
Bycroft,
NMR solution structure of a dsRNA binding domain from Drosophila staufen protein reveals homology to the N-terminal domain of ribosomal protein S5.
1995,
Pubmed
Corthésy,
Purification by DNA affinity chromatography of two polypeptides that contact the NF-AT DNA binding site in the interleukin 2 promoter.
1994,
Pubmed
Dong,
Microtubule binding to Smads may regulate TGF beta activity.
2000,
Pubmed
Dreyfuss,
hnRNP proteins and the biogenesis of mRNA.
1993,
Pubmed
Dubnau,
RNA recognition and translational regulation by a homeodomain protein.
1996,
Pubmed
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Duval,
Stability of maternal mRNA in Xenopus embryos: role of transcription and translation.
1990,
Pubmed
,
Xenbase
Eggert,
The glucocorticoid receptor is associated with the RNA-binding nuclear matrix protein hnRNP U.
1997,
Pubmed
Evan,
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
1985,
Pubmed
,
Xenbase
Ferrandon,
Staufen protein associates with the 3'UTR of bicoid mRNA to form particles that move in a microtubule-dependent manner.
1994,
Pubmed
Fleenor,
Comparison of human and Xenopus GATA-2 promoters.
1996,
Pubmed
,
Xenbase
Gatignol,
Characterization of a human TAR RNA-binding protein that activates the HIV-1 LTR.
1991,
Pubmed
Guille,
Microinjection into Xenopus oocytes and embryos.
1999,
Pubmed
,
Xenbase
Gurdon,
Injected nuclei in frog oocytes: fate, enlargement, and chromatin dispersal.
1976,
Pubmed
,
Xenbase
Guttridge,
Xenopus interspersed RNA families, Ocr and XR, bind DNA-binding proteins.
1995,
Pubmed
,
Xenbase
Hallier,
The transcription factor Spi-1/PU.1 binds RNA and interferes with the RNA-binding protein p54nrb.
1996,
Pubmed
Hyman,
Translational inactivation of ribosomal protein mRNAs during Xenopus oocyte maturation.
1988,
Pubmed
,
Xenbase
Kao,
Cloning and expression of cyclosporin A- and FK506-sensitive nuclear factor of activated T-cells: NF45 and NF90.
1994,
Pubmed
Klein,
Induction of mesoderm in Xenopus laevis embryos by translation initiation factor 4E.
1994,
Pubmed
,
Xenbase
Krovat,
Comparative mutational analysis of the double-stranded RNA binding domains of Xenopus laevis RNA-binding protein A.
1996,
Pubmed
,
Xenbase
Lee,
A testis cytoplasmic RNA-binding protein that has the properties of a translational repressor.
1996,
Pubmed
Li,
The association of Xenopus nuclear factor 7 with subcellular structures is dependent upon phosphorylation and specific domains.
1994,
Pubmed
,
Xenbase
Li,
Cytoplasmic retention of Xenopus nuclear factor 7 before the mid blastula transition uses a unique anchoring mechanism involving a retention domain and several phosphorylation sites.
1994,
Pubmed
,
Xenbase
Minegishi,
Alternative promoters regulate transcription of the mouse GATA-2 gene.
1998,
Pubmed
,
Xenbase
Moore,
Preparation and testing of synthetic mRNA for microinjection.
1999,
Pubmed
,
Xenbase
Murray,
Sequence analysis of cytoplasmic mRNA-binding proteins of Xenopus oocytes identifies a family of RNA-binding proteins.
1992,
Pubmed
,
Xenbase
Murray,
Different forms of soluble cytoplasmic mRNA binding proteins and particles in Xenopus laevis oocytes and embryos.
1991,
Pubmed
,
Xenbase
Nony,
Alternate promoters and developmental modulation of expression of the chicken GATA-2 gene in hematopoietic progenitor cells.
1998,
Pubmed
,
Xenbase
Olmedo,
Novel mutations that alter the regulation of sporulation in Bacillus subtilis. Evidence that phosphorylation of regulatory protein SpoOA controls the initiation of sporulation.
1990,
Pubmed
Orford,
The maternal CCAAT box transcription factor which controls GATA-2 expression is novel and developmentally regulated and contains a double-stranded-RNA-binding subunit.
1998,
Pubmed
,
Xenbase
Partington,
GATA-2 is a maternal transcription factor present in Xenopus oocytes as a nuclear complex which is maintained throughout early development.
1997,
Pubmed
,
Xenbase
Read,
The DNA sequence specificity of HMG boxes lies in the minor wing of the structure.
1994,
Pubmed
Rivera-Pomar,
RNA binding and translational suppression by bicoid.
1996,
Pubmed
Robbins,
Two interdependent basic domains in nucleoplasmin nuclear targeting sequence: identification of a class of bipartite nuclear targeting sequence.
1991,
Pubmed
,
Xenbase
Rudt,
Cytoplasmic retention and nuclear import of 5S ribosomal RNA containing RNPs.
1996,
Pubmed
,
Xenbase
Schumacher,
Spnr, a murine RNA-binding protein that is localized to cytoplasmic microtubules.
1995,
Pubmed
Smith,
Dorsalization and neural induction: properties of the organizer in Xenopus laevis.
1983,
Pubmed
,
Xenbase
St Johnston,
Multiple steps in the localization of bicoid RNA to the anterior pole of the Drosophila oocyte.
1989,
Pubmed
St Johnston,
Staufen, a gene required to localize maternal RNAs in the Drosophila egg.
1991,
Pubmed
Sykes,
Suppression of GATA factor activity causes axis duplication in Xenopus.
1998,
Pubmed
,
Xenbase
Tafuri,
Selective recruitment of masked maternal mRNA from messenger ribonucleoprotein particles containing FRGY2 (mRNP4).
1993,
Pubmed
,
Xenbase
Tsai,
An early haematopoietic defect in mice lacking the transcription factor GATA-2.
1994,
Pubmed
Tsai,
Transcription factor GATA-2 is required for proliferation/survival of early hematopoietic cells and mast cell formation, but not for erythroid and myeloid terminal differentiation.
1997,
Pubmed
Vandromme,
Regulation of transcription factor localization: fine-tuning of gene expression.
1996,
Pubmed
Walmsley,
Negative control of Xenopus GATA-2 by activin and noggin with eventual expression in precursors of the ventral blood islands.
1994,
Pubmed
,
Xenbase
Whiteside,
Signal transduction and nuclear targeting: regulation of transcription factor activity by subcellular localisation.
1993,
Pubmed
Wormington,
Preparation of synthetic mRNAs and analyses of translational efficiency in microinjected Xenopus oocytes.
1991,
Pubmed
,
Xenbase
Wormington,
Overexpression of poly(A) binding protein prevents maturation-specific deadenylation and translational inactivation in Xenopus oocytes.
1996,
Pubmed
,
Xenbase
Wormington,
Developmental expression and 5S rRNA-binding activity of Xenopus laevis ribosomal protein L5.
1989,
Pubmed
,
Xenbase
Yang,
JAZ requires the double-stranded RNA-binding zinc finger motifs for nuclear localization.
1999,
Pubmed
Ye,
Regulation of WT1 by phosphorylation: inhibition of DNA binding, alteration of transcriptional activity and cellular translocation.
1996,
Pubmed
Yurkova,
A translation regulatory particle containing the Xenopus oocyte Y box protein mRNP3+4.
1997,
Pubmed
,
Xenbase
Zhou,
Rescue of the embryonic lethal hematopoietic defect reveals a critical role for GATA-2 in urogenital development.
1998,
Pubmed
Zon,
Expression of GATA-binding proteins during embryonic development in Xenopus laevis.
1991,
Pubmed
,
Xenbase