Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
2003 Feb 03;223:395-403. doi: 10.1093/emboj/cdg035.
Show Gene links
Show Anatomy links
C-terminal domains implicated in the functional surface expression of potassium channels.
Jenke M
,
Sánchez A
,
Monje F
,
Stühmer W
,
Weseloh RM
,
Pardo LA
.
???displayArticle.abstract???
A short C-terminal domain is required for correct tetrameric assembly in some potassium channels. Here, we show that this domain forms a coiled coil that determines not only the stability but also the selectivity of the multimerization. Synthetic peptides comprising the sequence of this domain in Eag1 and other channels are able to form highly stable tetrameric coiled coils and display selective heteromultimeric interactions. We show that loss of function caused by disruption of this domain in Herg1 can be rescued by introducing the equivalent domain from Eag1, and that this chimeric protein can form heteromultimers with Eag1 while wild-type Erg1 cannot. Additionally, a short endoplasmic reticulum retention sequence closely preceding the coiled coil plays a crucial role for surface expression. Both domains appear to co-operate to form fully functional channels on the cell surface and are a frequent finding in ion channels. Many pathological phenotypes may be attributed to mutations affecting one or both domains.
Berggrun,
Contributions of distinct quaternary contacts to cooperative operator binding by Mnt repressor.
2001, Pubmed
Berggrun,
Contributions of distinct quaternary contacts to cooperative operator binding by Mnt repressor.
2001,
Pubmed
Biervert,
A potassium channel mutation in neonatal human epilepsy.
1998,
Pubmed
,
Xenbase
Böhm,
Quantitative analysis of protein far UV circular dichroism spectra by neural networks.
1992,
Pubmed
Brüggemann,
Ether-à-go-go encodes a voltage-gated channel permeable to K+ and Ca2+ and modulated by cAMP.
1993,
Pubmed
,
Xenbase
Cabezón,
The structure of bovine IF(1), the regulatory subunit of mitochondrial F-ATPase.
2001,
Pubmed
Cabezón,
Dimerization of bovine F1-ATPase by binding the inhibitor protein, IF1.
2000,
Pubmed
Charlier,
A pore mutation in a novel KQT-like potassium channel gene in an idiopathic epilepsy family.
1998,
Pubmed
Chen,
Polycystin-L is a calcium-regulated cation channel permeable to calcium ions.
1999,
Pubmed
,
Xenbase
Chen,
Sh and eag K(+) channel subunit interaction in frog oocytes depends on level and time of expression.
2000,
Pubmed
,
Xenbase
Chen,
Heteromultimeric interactions among K+ channel subunits from Shaker and eag families in Xenopus oocytes.
1996,
Pubmed
,
Xenbase
Clapham,
The TRP ion channel family.
2001,
Pubmed
Cohen,
Alpha-helical coiled coils and bundles: how to design an alpha-helical protein.
1990,
Pubmed
Cupers,
Parallel dimers and anti-parallel tetramers formed by epidermal growth factor receptor pathway substrate clone 15.
1997,
Pubmed
Engeland,
Cloning and functional expression of rat ether-à-go-go-like K+ channel genes.
1998,
Pubmed
Feng,
ASD4, a new GATA factor of Neurospora crassa, displays sequence-specific DNA binding and functions in ascus and ascospore development.
2000,
Pubmed
Fusetti,
Structure of tetrameric human phenylalanine hydroxylase and its implications for phenylketonuria.
1998,
Pubmed
Harbury,
A switch between two-, three-, and four-stranded coiled coils in GCN4 leucine zipper mutants.
1993,
Pubmed
Hufton,
Structure/function analysis of the domains required for the multimerisation of phenylalanine hydroxylase.
1998,
Pubmed
Ishii,
A human intermediate conductance calcium-activated potassium channel.
1997,
Pubmed
,
Xenbase
Kaupp,
The cyclic nucleotide-gated channels of vertebrate photoreceptors and olfactory epithelium.
1991,
Pubmed
Köhler,
Small-conductance, calcium-activated potassium channels from mammalian brain.
1996,
Pubmed
,
Xenbase
Kreusch,
Crystal structure of the tetramerization domain of the Shaker potassium channel.
1998,
Pubmed
Krieg,
In vitro RNA synthesis with SP6 RNA polymerase.
1987,
Pubmed
Kubisch,
KCNQ4, a novel potassium channel expressed in sensory outer hair cells, is mutated in dominant deafness.
1999,
Pubmed
,
Xenbase
Kupershmidt,
A K+ channel splice variant common in human heart lacks a C-terminal domain required for expression of rapidly activating delayed rectifier current.
1998,
Pubmed
Kupershmidt,
Defective human Ether-à-go-go-related gene trafficking linked to an endoplasmic reticulum retention signal in the C terminus.
2002,
Pubmed
Leclerc,
The open reading frame III product of cauliflower mosaic virus forms a tetramer through a N-terminal coiled-coil.
1998,
Pubmed
Li,
Specification of subunit assembly by the hydrophilic amino-terminal domain of the Shaker potassium channel.
1992,
Pubmed
Ludwig,
Functional expression of a rat homologue of the voltage gated either á go-go potassium channel reveals differences in selectivity and activation kinetics between the Drosophila channel and its mammalian counterpart.
1994,
Pubmed
,
Xenbase
Ludwig,
Carboxy-terminal domain mediates assembly of the voltage-gated rat ether-à-go-go potassium channel.
1997,
Pubmed
Lupas,
Predicting coiled coils from protein sequences.
1991,
Pubmed
Lupas,
Coiled coils: new structures and new functions.
1996,
Pubmed
Minor,
The polar T1 interface is linked to conformational changes that open the voltage-gated potassium channel.
2000,
Pubmed
,
Xenbase
O'Brien,
Probing the structure of rotavirus NSP4: a short sequence at the extreme C terminus mediates binding to the inner capsid particle.
2000,
Pubmed
Okamoto,
Multiple distinct coiled-coils are involved in dynamin self-assembly.
1999,
Pubmed
O'Neil,
A thermodynamic scale for the helix-forming tendencies of the commonly occurring amino acids.
1990,
Pubmed
O'Shea,
Evidence that the leucine zipper is a coiled coil.
1989,
Pubmed
Peng,
Reconstitution of the KRAB-KAP-1 repressor complex: a model system for defining the molecular anatomy of RING-B box-coiled-coil domain-mediated protein-protein interactions.
2000,
Pubmed
Schmitt,
A recessive C-terminal Jervell and Lange-Nielsen mutation of the KCNQ1 channel impairs subunit assembly.
2000,
Pubmed
,
Xenbase
Splawski,
Spectrum of mutations in long-QT syndrome genes. KVLQT1, HERG, SCN5A, KCNE1, and KCNE2.
2000,
Pubmed
Stavolone,
Tetramerization is a conserved feature of the virion-associated protein in plant pararetroviruses.
2001,
Pubmed
Stühmer,
Electrophysiological recording from Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Tang,
Shaker and ether-à-go-go K+ channel subunits fail to coassemble in Xenopus oocytes.
1998,
Pubmed
,
Xenbase
Terlau,
Extracellular Mg2+ regulates activation of rat eag potassium channel.
1996,
Pubmed
,
Xenbase
Trudeau,
HERG, a human inward rectifier in the voltage-gated potassium channel family.
1995,
Pubmed
Warmke,
A family of potassium channel genes related to eag in Drosophila and mammals.
1994,
Pubmed
Watanabe,
Characterization of the CIN85 adaptor protein and identification of components involved in CIN85 complexes.
2000,
Pubmed
Wimmers,
Erg1, erg2 and erg3 K channel subunits are able to form heteromultimers.
2001,
Pubmed
Zerangue,
An artificial tetramerization domain restores efficient assembly of functional Shaker channels lacking T1.
2000,
Pubmed
,
Xenbase
Zerangue,
A new ER trafficking signal regulates the subunit stoichiometry of plasma membrane K(ATP) channels.
1999,
Pubmed
,
Xenbase
Zhong,
Alteration of four identified K+ currents in Drosophila muscle by mutations in eag.
1991,
Pubmed
Zhong,
Modulation of different K+ currents in Drosophila: a hypothetical role for the Eag subunit in multimeric K+ channels.
1993,
Pubmed
