Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
1993 Aug 01;9015:7250-4. doi: 10.1073/pnas.90.15.7250.
Show Gene links
Show Anatomy links
Xenopus Ro ribonucleoproteins: members of an evolutionarily conserved class of cytoplasmic ribonucleoproteins.
O'Brien CA
,
Margelot K
,
Wolin SL
.
???displayArticle.abstract???
Ro small ribonucleoproteins consist of a 60-kDa protein and possibly additional proteins complexed with several small RNA molecules. The RNA components of these particles, designated Y RNAs, are about 100 nt long. Although these small ribonucleoproteins are abundant components of a variety of vertebrate species and cell types, their subcellular location is controversial, and their function is completely unknown. We have identified and characterized the Ro RNPs of Xenopus laevis. Three of the four distinct Xenopus Y RNAs appear to be related to the previously sequenced human hY3, hY4, and hY5 RNAs. The fourth Xenopus Y RNA, xY alpha, does not appear to be a homologue of any of the human Y RNAs. Each of the human and Xenopus Y RNAs possesses a conserved stem that contains the binding site for the 60-kDa Ro protein. Xenopus and human 60-kDa Ro proteins are 78% identical in amino acid sequence, with the conservation extending throughout the entire protein. When human hY3 RNA is mixed with Xenopus egg extracts, the human RNA assembles with the Xenopus Ro protein to form chimeric Ro ribonucleoproteins. By analyzing RNA extracted from manually enucleated oocytes and germinal vesicles, we have determined that Y RNAs are located in the oocytecytoplasm. By examining the distribution of mouse Ro ribonucleoproteins in cytoplast and karyoplast fractions derived from L-929 cells, we have determined that Ro ribonucleoprotein particles also primarily reside in the cytoplasm of mammalian cells.
Alspaugh,
Antibodies to cellular antigens in Sjögren's syndrome.
1975, Pubmed
Alspaugh,
Antibodies to cellular antigens in Sjögren's syndrome.
1975,
Pubmed
Baserga,
An intact Box C sequence in the U3 snRNA is required for binding of fibrillarin, the protein common to the major family of nucleolar snRNPs.
1991,
Pubmed
Ben-Chetrit,
A 52-kD protein is a novel component of the SS-A/Ro antigenic particle.
1988,
Pubmed
Deutscher,
Molecular analysis of the 60-kDa human Ro ribonucleoprotein.
1988,
Pubmed
Donis-Keller,
Phy M: an RNase activity specific for U and A residues useful in RNA sequence analysis.
1980,
Pubmed
Donis-Keller,
Mapping adenines, guanines, and pyrimidines in RNA.
1977,
Pubmed
Gurney,
Intracellular distribution of low molecular weight RNA species in HeLa cells.
1980,
Pubmed
Harmon,
The importance of tissue substrate in the SS-A/Ro antigen-antibody system.
1984,
Pubmed
Hendrick,
Ro small cytoplasmic ribonucleoproteins are a subclass of La ribonucleoproteins: further characterization of the Ro and La small ribonucleoproteins from uninfected mammalian cells.
1981,
Pubmed
Kato,
Nucleotide sequence of 4.5S RNA (C8 or hY5) from HeLa cells.
1982,
Pubmed
Kenan,
RNA recognition: towards identifying determinants of specificity.
1991,
Pubmed
Kleinschmidt,
Molecular characterization of a karyophilic, histone-binding protein: cDNA cloning, amino acid sequence and expression of nuclear protein N1/N2 of Xenopus laevis.
1986,
Pubmed
,
Xenbase
Krupp,
Rapid RNA sequencing: nucleases from Staphylococcus aureus and Neurospora crassa discriminate between uridine and cytidine.
1979,
Pubmed
Kunkel,
RNA polymerase III transcription of genes that lack internal control regions.
1991,
Pubmed
,
Xenbase
Lerner,
Two novel classes of small ribonucleoproteins detected by antibodies associated with lupus erythematosus.
1981,
Pubmed
Lund,
In vitro synthesis of vertebrate U1 snRNA.
1989,
Pubmed
,
Xenbase
Mamula,
The Ro ribonucleoprotein particle: induction of autoantibodies and the detection of Ro RNAs among species.
1989,
Pubmed
Mattioli,
Heterogeneity of RNA protein antigens reactive with sera of patients with systemic lupus erythematosus. Description of a cytoplasmic nonribosomal antigen.
1974,
Pubmed
Monroy,
Characteristics of reactions catalyzed by purified guanylyltransferase from vaccinia virus.
1978,
Pubmed
Murray,
Cyclin synthesis drives the early embryonic cell cycle.
1989,
Pubmed
,
Xenbase
O'Brien,
A subset of hY RNAs is associated with erythrocyte Ro ribonucleoproteins.
1990,
Pubmed
Ochman,
Genetic applications of an inverse polymerase chain reaction.
1988,
Pubmed
Pruijn,
Analysis of protein--RNA interactions within Ro ribonucleoprotein complexes.
1991,
Pubmed
Reddy,
Detection of a nucleolar 7-2 ribonucleoprotein and a cytoplasmic 8-2 ribonucleoprotein with autoantibodies from patients with scleroderma.
1983,
Pubmed
Richter,
Gene expression in the embryonic nervous system of Xenopus laevis.
1988,
Pubmed
,
Xenbase
Stefano,
Purified lupus antigen La recognizes an oligouridylate stretch common to the 3' termini of RNA polymerase III transcripts.
1984,
Pubmed
Toczyski,
The cellular RNA-binding protein EAP recognizes a conserved stem-loop in the Epstein-Barr virus small RNA EBER 1.
1993,
Pubmed
Wigler,
A preparative method for obtaining enucleated mammalian cells.
1975,
Pubmed
Wolin,
The Ro small cytoplasmic ribonucleoproteins: identification of the antigenic protein and its binding site on the Ro RNAs.
1984,
Pubmed
Wolin,
Genes for two small cytoplasmic Ro RNAs are adjacent and appear to be single-copy in the human genome.
1983,
Pubmed
Yisraeli,
Synthesis of long, capped transcripts in vitro by SP6 and T7 RNA polymerases.
1989,
Pubmed
Zieve,
Newly synthesized small nuclear RNAs appear transiently in the cytoplasm.
1988,
Pubmed
