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Regulation of quantal secretion by neurotrophic factors at developing motoneurons in Xenopus cell cultures.
Liou JC
,
Yang RS
,
Fu WM
.
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1. The ability of different neurotrophic factors to maintain and regulate synaptic function at the developing motoneuron was studied in Xenopus nerve-muscle co-cultures. Spontaneous synaptic currents (SSCs) were measured by using whole-cell voltage-clamped myocytes. 2. Compared with natural synapses, motoneurons without contact on a myocyte (naive neurons) released ACh in smaller quantal packets, the amplitude being inversely proportional to the days in culture. The mean SSC amplitudes of naive neurons, which were measured by manipulating a myoball into contact with the myocyte-free nerve terminals to form a manipulated synapse, were 99.5 +/- 6.7 and 48.2 +/- 1.9 pA for day-1 and day-3 cultures, respectively. 3. Chronic treatment of day-1 cultures with brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), neurotrophin-4 (NT-4), ciliary neurotrophic factor (CNTF) or glial cell line-derived neurotrophic factor (GDNF) for 2 days, increased the ACh quantal size of naive motoneurons in a concentration-dependent manner, whereas insulin-like growth factor-1 (IGF-1) and basic fibroblast growth factor (bFGF) had no effect, even at high concentrations. 4. The interaction of various neurotrophic factors was examined, using concentrations that gave maximal effects. Combination of CNTF plus BDNF or CNTF plus NT-3 had synergistic effects in potentiating SSC amplitude of the manipulated synapse of naive neurons, whereas NT-3 plus BDNF, NT-3 plus GDNF, BDNF plus GDNF or CNTF plus GDNF had no synergistic action. 5. Chronic treatment with d-tubocurarine for 2 days resulted in a reduction of the quantal size of natural synapses. Concomitant treatment with BDNF, NT-3, GDNF, CNTF but not bFGF or IGF-1, reconstituted the SSC amplitude. 6. Taken together, these findings suggest that BDNF, NT-3, NT-4, CNTF and GDNF may regulate and maintain the synaptic function of developing motoneurons, and different neurotrophic factors utilizing distinct signalling mechanisms may have synergistic actions.
Arakawa,
Survival effect of ciliary neurotrophic factor (CNTF) on chick embryonic motoneurons in culture: comparison with other neurotrophic factors and cytokines.
1990, Pubmed
Arakawa,
Survival effect of ciliary neurotrophic factor (CNTF) on chick embryonic motoneurons in culture: comparison with other neurotrophic factors and cytokines.
1990,
Pubmed
Bothwell,
Functional interactions of neurotrophins and neurotrophin receptors.
1995,
Pubmed
Caroni,
Activity-sensitive signaling by muscle-derived insulin-like growth factors in the developing and regenerating neuromuscular system.
1993,
Pubmed
Chapman,
Cost of mating in Drosophila melanogaster females is mediated by male accessory gland products.
1995,
Pubmed
Dan,
Retrograde interactions during formation and elimination of neuromuscular synapses.
1994,
Pubmed
Hall,
Synaptic structure and development: the neuromuscular junction.
1993,
Pubmed
Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed
Henderson,
GDNF: a potent survival factor for motoneurons present in peripheral nerve and muscle.
1994,
Pubmed
Henderson,
Role of neurotrophic factors in neuronal development.
1996,
Pubmed
Henderson,
Neurotrophins promote motor neuron survival and are present in embryonic limb bud.
1993,
Pubmed
Heumann,
Neurotrophin signalling.
1994,
Pubmed
Ikeda,
Basic fibroblast growth factor has neuroprotective effects on axotomy-induced spinal motoneuron death and wobbler mouse motoneuron disease.
1996,
Pubmed
Jones,
Targeted disruption of the BDNF gene perturbs brain and sensory neuron development but not motor neuron development.
1994,
Pubmed
Kidokoro,
Early cross-striation formation in twitching Xenopus myocytes in culture.
1988,
Pubmed
,
Xenbase
Klein,
Disruption of the neurotrophin-3 receptor gene trkC eliminates la muscle afferents and results in abnormal movements.
1994,
Pubmed
Koliatsos,
Evidence that brain-derived neurotrophic factor is a trophic factor for motor neurons in vivo.
1993,
Pubmed
Korsching,
The neurotrophic factor concept: a reexamination.
1993,
Pubmed
Lin,
GDNF: a glial cell line-derived neurotrophic factor for midbrain dopaminergic neurons.
1993,
Pubmed
Liou,
Regulation of quantal secretion from developing motoneurons by postsynaptic activity-dependent release of NT-3.
1997,
Pubmed
,
Xenbase
McManaman,
Rescue of motoneurons from cell death by a purified skeletal muscle polypeptide: effects of the ChAT development factor, CDF.
1990,
Pubmed
Mitsumoto,
Arrest of motor neuron disease in wobbler mice cotreated with CNTF and BDNF.
1994,
Pubmed
Oppenheim,
Control of embryonic motoneuron survival in vivo by ciliary neurotrophic factor.
1991,
Pubmed
Pennica,
Cardiotrophin-1, a cytokine present in embryonic muscle, supports long-term survival of spinal motoneurons.
1996,
Pubmed
Schecterson,
Novel roles for neurotrophins are suggested by BDNF and NT-3 mRNA expression in developing neurons.
1992,
Pubmed
Segal,
Intracellular signaling pathways activated by neurotrophic factors.
1996,
Pubmed
Sendtner,
Brain-derived neurotrophic factor prevents the death of motoneurons in newborn rats after nerve section.
,
Pubmed
Stoop,
Synaptic modulation by neurotrophic factors: differential and synergistic effects of brain-derived neurotrophic factor and ciliary neurotrophic factor.
1996,
Pubmed
,
Xenbase
Trupp,
Peripheral expression and biological activities of GDNF, a new neurotrophic factor for avian and mammalian peripheral neurons.
1995,
Pubmed
Wong,
The neurotrophins BDNF, NT-3 and NT-4/5, but not NGF, up-regulate the cholinergic phenotype of developing motor neurons.
1993,
Pubmed
Xie,
Initial events in the formation of neuromuscular synapse: rapid induction of acetylcholine release from embryonic neuron.
1986,
Pubmed
,
Xenbase
Yan,
Influences of neurotrophins on mammalian motoneurons in vivo.
1993,
Pubmed
Zafra,
Regulation of brain-derived neurotrophic factor and nerve growth factor mRNA in primary cultures of hippocampal neurons and astrocytes.
1992,
Pubmed
