Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Biol Chem
2008 May 30;28322:15072-7. doi: 10.1074/jbc.M706964200.
Show Gene links
Show Anatomy links
I SA channel complexes include four subunits each of DPP6 and Kv4.2.
Soh H
,
Goldstein SA
.
???displayArticle.abstract???
Kv4 potassium channels produce rapidly inactivating currents that regulate excitability of muscles and nerves. To reconstitute the neuronal A-type current I(SA), Kv4 subunits assemble with DPP6, a single transmembrane domain accessory subunit. DPP6 alters function-accelerating activation, inactivation, and recovery from inactivation-and increases surface expression. We sought here to determine the stoichiometry of Kv4 and DPP6 in complexes using functional and biochemical methods. First, wild type channels formed from subunit monomers were compared with channels carrying subunits linked in tandem to enforce 4:4 and 4:2 assemblies (Kv4.2-DPP6 and Kv4.2-Kv4.2-DPP6). Next, channels were overexpressed and purified so that the molar ratio of subunits in complexes could be assessed by direct amino acid analysis. Both biophysical and biochemical methods indicate that I(SA) channels carry four subunits each of Kv4.2 and DPP6.
Abbott,
A superfamily of small potassium channel subunits: form and function of the MinK-related peptides (MiRPs).
1998, Pubmed
Abbott,
A superfamily of small potassium channel subunits: form and function of the MinK-related peptides (MiRPs).
1998,
Pubmed
An,
Modulation of A-type potassium channels by a family of calcium sensors.
2000,
Pubmed
,
Xenbase
Brahmajothi,
Distinct transient outward potassium current (Ito) phenotypes and distribution of fast-inactivating potassium channel alpha subunits in ferret left ventricular myocytes.
1999,
Pubmed
Chen,
Charybdotoxin binding in the I(Ks) pore demonstrates two MinK subunits in each channel complex.
2003,
Pubmed
,
Xenbase
Clay,
Determining K+ channel activation curves from K+ channel currents.
2000,
Pubmed
,
Xenbase
Clement,
Association and stoichiometry of K(ATP) channel subunits.
1997,
Pubmed
Ding,
Inactivating BK channels in rat chromaffin cells may arise from heteromultimeric assembly of distinct inactivation-competent and noninactivating subunits.
1998,
Pubmed
Dixon,
Role of the Kv4.3 K+ channel in ventricular muscle. A molecular correlate for the transient outward current.
1996,
Pubmed
Dougherty,
A dipeptidyl aminopeptidase-like protein remodels gating charge dynamics in Kv4.2 channels.
2006,
Pubmed
,
Xenbase
Ficker,
Role of the cytosolic chaperones Hsp70 and Hsp90 in maturation of the cardiac potassium channel HERG.
2003,
Pubmed
Fiset,
Shal-type channels contribute to the Ca2+-independent transient outward K+ current in rat ventricle.
1997,
Pubmed
Gulbis,
Structure of the cytoplasmic beta subunit-T1 assembly of voltage-dependent K+ channels.
2000,
Pubmed
,
Xenbase
Hinzpeter,
Association between Hsp90 and the ClC-2 chloride channel upregulates channel function.
2006,
Pubmed
Jentsch,
Molecular structure and physiological function of chloride channels.
2002,
Pubmed
Jerng,
Multiprotein assembly of Kv4.2, KChIP3 and DPP10 produces ternary channel complexes with ISA-like properties.
2005,
Pubmed
,
Xenbase
Jerng,
Modulation of Kv4.2 channel expression and gating by dipeptidyl peptidase 10 (DPP10).
2004,
Pubmed
,
Xenbase
Jerng,
K+ channel inactivation mediated by the concerted action of the cytoplasmic N- and C-terminal domains.
1997,
Pubmed
,
Xenbase
Johns,
Suppression of neuronal and cardiac transient outward currents by viral gene transfer of dominant-negative Kv4.2 constructs.
1997,
Pubmed
Kääb,
Molecular basis of transient outward potassium current downregulation in human heart failure: a decrease in Kv4.3 mRNA correlates with a reduction in current density.
1998,
Pubmed
Kim,
Three-dimensional structure of I(to); Kv4.2-KChIP2 ion channels by electron microscopy at 21 Angstrom resolution.
2004,
Pubmed
Kim,
Ito channels are octomeric complexes with four subunits of each Kv4.2 and K+ channel-interacting protein 2.
2004,
Pubmed
,
Xenbase
Knaus,
Primary sequence and immunological characterization of beta-subunit of high conductance Ca(2+)-activated K+ channel from smooth muscle.
1994,
Pubmed
Liss,
Tuning pacemaker frequency of individual dopaminergic neurons by Kv4.3L and KChip3.1 transcription.
2001,
Pubmed
Malin,
Elimination of the fast transient in superior cervical ganglion neurons with expression of KV4.2W362F: molecular dissection of IA.
2000,
Pubmed
Mason,
Modulation of Kv1.5 currents by protein kinase A, tyrosine kinase, and protein tyrosine phosphatase requires an intact cytoskeleton.
2002,
Pubmed
,
Xenbase
Melman,
KCNE1 binds to the KCNQ1 pore to regulate potassium channel activity.
2004,
Pubmed
Morin,
Counting membrane-embedded KCNE beta-subunits in functioning K+ channel complexes.
2008,
Pubmed
,
Xenbase
Nadal,
Evidence for the presence of a novel Kv4-mediated A-type K(+) channel-modifying factor.
2001,
Pubmed
,
Xenbase
Nadal,
The CD26-related dipeptidyl aminopeptidase-like protein DPPX is a critical component of neuronal A-type K+ channels.
2003,
Pubmed
,
Xenbase
Petrecca,
Localization and enhanced current density of the Kv4.2 potassium channel by interaction with the actin-binding protein filamin.
2000,
Pubmed
Pioletti,
Three-dimensional structure of the KChIP1-Kv4.3 T1 complex reveals a cross-shaped octamer.
2006,
Pubmed
,
Xenbase
Ren,
Transmembrane interaction mediates complex formation between peptidase homologues and Kv4 channels.
2005,
Pubmed
Rhodes,
Association and colocalization of the Kvbeta1 and Kvbeta2 beta-subunits with Kv1 alpha-subunits in mammalian brain K+ channel complexes.
1997,
Pubmed
Sampson,
Direct interaction between the actin-binding protein filamin-A and the inwardly rectifying potassium channel, Kir2.1.
2003,
Pubmed
Shibata,
A fundamental role for KChIPs in determining the molecular properties and trafficking of Kv4.2 potassium channels.
2003,
Pubmed
Shyng,
Octameric stoichiometry of the KATP channel complex.
1997,
Pubmed
Sokolova,
Three-dimensional structure of a voltage-gated potassium channel at 2.5 nm resolution.
2001,
Pubmed
Strop,
Structure of a human A-type potassium channel interacting protein DPPX, a member of the dipeptidyl aminopeptidase family.
2004,
Pubmed
Tai,
The conduction pore of a cardiac potassium channel.
1998,
Pubmed
,
Xenbase
Wang,
Consequences of the stoichiometry of Slo1 alpha and auxiliary beta subunits on functional properties of large-conductance Ca2+-activated K+ channels.
2002,
Pubmed
,
Xenbase
Wang,
Structural basis for modulation of Kv4 K+ channels by auxiliary KChIP subunits.
2007,
Pubmed
,
Xenbase
Wang,
Subunit composition of minK potassium channels.
1995,
Pubmed
,
Xenbase
Zagha,
DPP10 modulates Kv4-mediated A-type potassium channels.
2005,
Pubmed
