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Chien YH
,
Werner ME
,
Stubbs J
,
Joens MS
,
Li J
,
Chien S
,
Fitzpatrick JA
,
Mitchell BJ
,
Kintner C
.
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Multiciliate cells (MCCs) are highly specialized epithelial cells that employ hundreds of motile cilia to produce a vigorous directed flow in a variety of organ systems. The production of this flow requires the establishment of planar cell polarity (PCP) whereby MCCs align hundreds of beating cilia along a common planar axis. The planar axis of cilia in MCCs is known to be established via the PCP pathway and hydrodynamic cues, but the downstream steps required for cilia orientation remain poorly defined. Here, we describe a new component of cilia orientation, based on the phenotypic analysis of an uncharacterized coiled-coil protein, called bbof1. We show that the expression of bbof1 is induced during the early phases of MCC differentiation by the master regulator foxj1. MCC differentiation and ciliogenesis occurs normally in embryos where bbof1 activity is reduced, but cilia orientation is severely disrupted. We show that cilia in bbof1 mutants can still respond to patterning and hydrodynamic cues, but lack the ability to maintain their precise orientation. Misexpression of bbof1 promotes cilia alignment, even in the absence of flow or in embryos where microtubules and actin filaments are disrupted. Bbof1 appears to mediate cilia alignment by localizing to a polar structure adjacent to the basal body. Together, these results suggest that bbof1 is a basal body component required in MCCs to align and maintain cilia orientation in response to flow.
Bayly,
Pointing in the right direction: new developments in the field of planar cell polarity.
2011, Pubmed
Bayly,
Pointing in the right direction: new developments in the field of planar cell polarity.
2011,
Pubmed
Deblandre,
A two-step mechanism generates the spacing pattern of the ciliated cells in the skin of Xenopus embryos.
1999,
Pubmed
,
Xenbase
Gordon,
Three-dimensional organization of microtubules and microfilaments of the basal body apparatus of ciliated respiratory epithelium.
1982,
Pubmed
Guirao,
Coupling between hydrodynamic forces and planar cell polarity orients mammalian motile cilia.
2010,
Pubmed
Kunimoto,
Coordinated ciliary beating requires Odf2-mediated polarization of basal bodies via basal feet.
2012,
Pubmed
Marshall,
Basal bodies platforms for building cilia.
2008,
Pubmed
Marshall,
Cilia orientation and the fluid mechanics of development.
2008,
Pubmed
Mitchell,
A positive feedback mechanism governs the polarity and motion of motile cilia.
2007,
Pubmed
,
Xenbase
Mitchell,
The PCP pathway instructs the planar orientation of ciliated cells in the Xenopus larval skin.
2009,
Pubmed
,
Xenbase
Momose,
A conserved function for Strabismus in establishing planar cell polarity in the ciliated ectoderm during cnidarian larval development.
2012,
Pubmed
Park,
Dishevelled controls apical docking and planar polarization of basal bodies in ciliated epithelial cells.
2008,
Pubmed
,
Xenbase
Stubbs,
Multicilin promotes centriole assembly and ciliogenesis during multiciliate cell differentiation.
2012,
Pubmed
,
Xenbase
Stubbs,
The forkhead protein Foxj1 specifies node-like cilia in Xenopus and zebrafish embryos.
2008,
Pubmed
,
Xenbase
Vladar,
Microtubules enable the planar cell polarity of airway cilia.
2012,
Pubmed
Wallingford,
Strange as it may seem: the many links between Wnt signaling, planar cell polarity, and cilia.
2011,
Pubmed
,
Xenbase
Werner,
Actin and microtubules drive differential aspects of planar cell polarity in multiciliated cells.
2011,
Pubmed
,
Xenbase
Werner,
Understanding ciliated epithelia: the power of Xenopus.
2012,
Pubmed
,
Xenbase
Yang,
The ciliary rootlet maintains long-term stability of sensory cilia.
2005,
Pubmed
Yang,
Rootletin, a novel coiled-coil protein, is a structural component of the ciliary rootlet.
2002,
Pubmed
Yang,
Rootletin interacts with C-Nap1 and may function as a physical linker between the pair of centrioles/basal bodies in cells.
2006,
Pubmed
Zallen,
Planar polarity and tissue morphogenesis.
2007,
Pubmed
