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Characterization of the hyperpolarization-activated chloride current in dissociated rat sympathetic neurons.
Clark S
,
Jordt SE
,
Jentsch TJ
,
Mathie A
.
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1. Dissociated rat superior cervical ganglion (SCG) neurons have been shown to possess a hyperpolarization-activated inwardly rectifying chloride current. The current was not altered by changes in external potassium concentration, replacing external cations with NMDG (N-methyl-D-glucamine) or by addition of 10 mM caesium or barium ions. 2. The reversal potential of the current was altered by changing external anions. The anion selectivity of the current was Cl- > Br- > I- > cyclamate. All substituted permeant anions also blocked the current. 3. The current was blocked by DIDS (4,4'-diisothiocyanatostilbene-2,2'-disulphonic acid), 9AC (anthracene-9-carboxylic acid) and NPPB (5-nitro-2-(3-phenylpropylamino)benzoic acid) but was unaffected by SITS (4-acetamido-4'-isothiocyanatostilbene- 2,2'-disulphonic acid) and niflumic acid. The effective blockers were voltage dependent; DIDS and NPPB were more effective at depolarized potentials while 9AC was more effective at hyperpolarized potentials. 4. The current was enhanced by extracellular acidification and reduced by extracellular alkalinization. Reducing external osmolarity was without effect in conventional whole-cell recording but enhanced current amplitude in those perforated-patch recordings where little current was evident in control external solution. 5. The current in SCG neurons was blocked by external cadmium and zinc. ClC-2 chloride currents expressed in Xenopus oocytes were also sensitive to block by these divalent ions and by DIDS but the sensitivity of ClC-2 to block by cadmium ions was lower than that of the current in SCG neurons. 6. Reverse transcriptase-polymerase chain reaction (RT-PCR) experiments showed the presence of mRNA for ClC-2 in SCG neurons but not in rat cerebellar granule cells which do not possess a hyperpolarization-activated Cl- current. 7. The data suggest that ClC-2 may be functionally expressed in rat SCG neurons. This current may play a role in regulating the internal chloride concentration in these neurons and hence their response to activation of GABAA receptors.
Ackerman,
Hypotonicity activates a native chloride current in Xenopus oocytes.
1994, Pubmed,
Xenbase
Ackerman,
Hypotonicity activates a native chloride current in Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Adams,
Actions of gamma-aminobutyric acid on sympathetic ganglion cells.
1975,
Pubmed
Anderson,
Chloride channels in the apical membrane of normal and cystic fibrosis airway and intestinal epithelia.
1992,
Pubmed
Arreola,
Volume-activated chloride channels in rat parotid acinar cells.
1995,
Pubmed
Arreola,
Three distinct chloride channels control anion movements in rat parotid acinar cells.
1996,
Pubmed
Bryant,
Chloride conductance in normal and myotonic muscle fibres and the action of monocarboxylic aromatic acids.
1971,
Pubmed
Chesnoy-Marchais,
Activation of hyperpolarization and atypical osmosensitivity of a Cl- current in rat osteoblastic cells.
1994,
Pubmed
Chesnoy-Marchais,
Characterization of a chloride conductance activated by hyperpolarization in Aplysia neurones.
1983,
Pubmed
Cid,
Cloning of a putative human voltage-gated chloride channel (CIC-2) cDNA widely expressed in human tissues.
1995,
Pubmed
Dinudom,
A forskolin-activated Cl- current in mouse mandibular duct cells.
1995,
Pubmed
Dobó,
Evidence for GABAergic fibers entering the superior cervical ganglion of rat from the preganglionic nerve trunk.
1989,
Pubmed
Eugène,
Fast non-cholinergic depolarizing postsynaptic potentials in neurons of rat superior cervical ganglia.
1987,
Pubmed
Ferroni,
Two distinct inwardly rectifying conductances are expressed in long term dibutyryl-cyclic-AMP treated rat cultured cortical astrocytes.
1995,
Pubmed
Frederickson,
Neurobiology of zinc and zinc-containing neurons.
1989,
Pubmed
Fritsch,
Osmosensitivity of the hyperpolarization-activated chloride current in human intestinal T84 cells.
1997,
Pubmed
,
Xenbase
Fritsch,
Modulation of the hyperpolarization-activated Cl- current in human intestinal T84 epithelial cells by phosphorylation.
1996,
Pubmed
Gründer,
Regions involved in the opening of CIC-2 chloride channel by voltage and cell volume.
,
Pubmed
,
Xenbase
Hall,
Cyclic AMP prevents activation of a swelling-induced chloride-sensitive conductance in chick heart cells.
1995,
Pubmed
Halliwell,
Voltage-clamp analysis of muscarinic excitation in hippocampal neurons.
1982,
Pubmed
Ide,
An anion channel from transverse tubular membranes incorporated into planar bilayers.
1995,
Pubmed
Jordt,
Molecular dissection of gating in the ClC-2 chloride channel.
1997,
Pubmed
,
Xenbase
Kowdley,
Hyperpolarization-activated chloride currents in Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Lamas,
Effects of a cognition-enhancer, linopirdine (DuP 996), on M-type potassium currents (IK(M)) and some other voltage- and ligand-gated membrane currents in rat sympathetic neurons.
1997,
Pubmed
Lorenz,
Heteromultimeric CLC chloride channels with novel properties.
1996,
Pubmed
,
Xenbase
Madison,
Phorbol esters block a voltage-sensitive chloride current in hippocampal pyramidal cells.
,
Pubmed
Moorman,
Phospholemman expression induces a hyperpolarization-activated chloride current in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Nilius,
Volume-activated Cl- currents in different mammalian non-excitable cell types.
1994,
Pubmed
Párducz,
Termination pattern and fine structural characteristics of GABA- and [Met]enkephalin-containing nerve fibers and synapses in the superior cervical ganglion of adult rat.
1992,
Pubmed
Pusch,
Molecular physiology of voltage-gated chloride channels.
1994,
Pubmed
Smith,
Differential expression of an inwardly rectifying chloride conductance in rat brain neurons: a potential mechanism for cell-specific modulation of postsynaptic inhibition.
1995,
Pubmed
Staley,
Alteration of GABAA receptor function following gene transfer of the CLC-2 chloride channel.
1996,
Pubmed
Staley,
The role of an inwardly rectifying chloride conductance in postsynaptic inhibition.
1994,
Pubmed
Steinmeyer,
Primary structure and functional expression of a developmentally regulated skeletal muscle chloride channel.
1991,
Pubmed
,
Xenbase
Thiemann,
A chloride channel widely expressed in epithelial and non-epithelial cells.
1992,
Pubmed
,
Xenbase
Watkins,
A non-inactivating K+ current sensitive to muscarinic receptor activation in rat cultured cerebellar granule neurons.
1996,
Pubmed
Wooltorton,
Potent block of potassium currents in rat isolated sympathetic neurones by the uncharged form of amitriptyline and related tricyclic compounds.
1995,
Pubmed
