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A novel amphibian Pi-class glutathione transferase isoenzyme from Xenopus laevis: importance of phenylalanine 111 in the H-site.
De Luca A
,
Favaloro B
,
Carletti E
,
Sacchetta P
,
Di Ilio C
.
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Screening of a liver tumour cDNA library from Xenopus laevis resulted in the isolation of a full-length cDNA clone encoding a novel Pi-class amphibian glutathione transferase (GST) isoenzyme (designated as XlGSTP1-1). The gene encodes a protein of 212 amino acids with a calculated molecular mass of 24428 Da. The product of the gene has been overexpressed in Escherichia coli and characterized. XlGSTP1-1 has one of the highest specific activities towards 1-chloro-2,4-dinitrobenzene (1310 micromol/min per mg of protein) obtained with any GST. A notable feature of XlGSTP1-1 is the presence in the H-site of Phe(111) and Pro(208) in place of tyrosine and glycine residues respectively, present in other mammalian Pi-class GSTs. Site-directed mutagenesis indicate that Phe(111) is involved in substrate specificity of XlGSTP1-1. We provide evidence showing that XlGSTP1-1 is present only in the embryo and its expression might be associated with cellular proliferation.
Aceto,
Developmental aspects of Bufo bufo embryo glutathione transferases.
1993,
Pubmed
Amicarelli,
Developmental expression and distribution of amphibian glutathione transferases.
2001,
Pubmed
Angelucci,
Glutathione transferase isoenzymes from frog (Xenopus laevis) liver and embryo.
2002,
Pubmed
,
Xenbase
Armstrong,
Structure, catalytic mechanism, and evolution of the glutathione transferases.
1997,
Pubmed
Bammler,
Amino acid differences at positions 10, 11, and 104 explain the profound catalytic differences between two murine pi-class glutathione S-transferases.
1995,
Pubmed
Bochner,
AppppA and related adenylylated nucleotides are synthesized as a consequence of oxidation stress.
1984,
Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Bucciarelli,
Characterization of toad liver glutathione transferase.
1999,
Pubmed
Bucciarelli,
Amino acid sequence of the major form of toad liver glutathione transferase.
2002,
Pubmed
Cho,
Glutathione S-transferase mu modulates the stress-activated signals by suppressing apoptosis signal-regulating kinase 1.
2001,
Pubmed
Del Boccio,
Interaction of glutathione transferase from horse erythrocytes with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole.
1991,
Pubmed
De Luca,
Mu-class glutathione transferase from Xenopus laevis: molecular cloning, expression and site-directed mutagenesis.
2002,
Pubmed
,
Xenbase
Di Ilio,
Glutathione transferase isoenzymes from Bufo bufo embryos at an early developmental stage.
1992,
Pubmed
Di Ilio,
Selenium independent glutathione peroxidase activity associated with cationic forms of glutathione transferase in human heart.
1986,
Pubmed
Dirr,
X-ray crystal structures of cytosolic glutathione S-transferases. Implications for protein architecture, substrate recognition and catalytic function.
1994,
Pubmed
Dirr,
Class pi glutathione S-transferase from pig lung. Purification, biochemical characterization, primary structure and crystallization.
1991,
Pubmed
Habig,
Assays for differentiation of glutathione S-transferases.
1981,
Pubmed
Hayes,
The glutathione S-transferase supergene family: regulation of GST and the contribution of the isoenzymes to cancer chemoprotection and drug resistance.
1995,
Pubmed
Kano,
Structure and expression of a human class pi glutathione S-transferase messenger RNA.
1987,
Pubmed
Kolm,
Isothiocyanates as substrates for human glutathione transferases: structure-activity studies.
1995,
Pubmed
Lardelli,
Generating bacteriophage lambda sublibraries enriched for rare clones.
1994,
Pubmed
Lee,
A molecular genetic approach for the identification of essential residues in human glutathione S-transferase function in Escherichia coli.
1995,
Pubmed
Lo Bello,
Multifunctional role of Tyr 108 in the catalytic mechanism of human glutathione transferase P1-1. Crystallographic and kinetic studies on the Y108F mutant enzyme.
1997,
Pubmed
Mannervik,
Glutathione transferases--structure and catalytic activity.
1988,
Pubmed
Mannervik,
Nomenclature for human glutathione transferases.
1992,
Pubmed
Mannervik,
The isoenzymes of glutathione transferase.
1985,
Pubmed
Oakley,
The three-dimensional structure of the human Pi class glutathione transferase P1-1 in complex with the inhibitor ethacrynic acid and its glutathione conjugate.
1997,
Pubmed
Reinemer,
Three-dimensional structure of class pi glutathione S-transferase from human placenta in complex with S-hexylglutathione at 2.8 A resolution.
1992,
Pubmed
Ricci,
Site-directed mutagenesis of human glutathione transferase P1-1. Mutation of Cys-47 induces a positive cooperativity in glutathione transferase P1-1.
1995,
Pubmed
Sacchetta,
Amphibian embryo glutathione transferase: amino acid sequence and structural properties.
1997,
Pubmed
Sheehan,
Structure, function and evolution of glutathione transferases: implications for classification of non-mammalian members of an ancient enzyme superfamily.
2001,
Pubmed
Simons,
Purification of glutathione S-transferases from human liver by glutathione-affinity chromatography.
1977,
Pubmed
Stanley,
The conjugation of 4-nitroquinoline 1-oxide, a potent carcinogen, by mammalian glutathione transferases. 4-Nitroquinoline 1-oxide conjugation by human, rat and mouse liver cytosols, extrahepatic organs of mice and purified mouse glutathione transferase isoenzymes.
1988,
Pubmed
Vega,
The three-dimensional structure of Cys-47-modified mouse liver glutathione S-transferase P1-1. Carboxymethylation dramatically decreases the affinity for glutathione and is associated with a loss of electron density in the alphaB-310B region.
1998,
Pubmed
Wang,
Glutathione S-transferase P1-1 (GSTP1-1) inhibits c-Jun N-terminal kinase (JNK1) signaling through interaction with the C terminus.
2001,
Pubmed
Xu,
Two murine GSTpi genes are arranged in tandem and are differentially expressed.
1994,
Pubmed
Zimniak,
Naturally occurring human glutathione S-transferase GSTP1-1 isoforms with isoleucine and valine in position 104 differ in enzymic properties.
1994,
Pubmed
