Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
1993 Nov 01;9021:10235-9.
Show Gene links
Show Anatomy links
Atrial G protein-activated K+ channel: expression cloning and molecular properties.
Dascal N
,
Schreibmayer W
,
Lim NF
,
Wang W
,
Chavkin C
,
DiMagno L
,
Labarca C
,
Kieffer BL
,
Gaveriaux-Ruff C
,
Trollinger D
.
???displayArticle.abstract???
Activity of several ion channels is controlled by heterotrimeric GTP-binding proteins (G proteins) via a membrane-delimited pathway that does not involve cytoplasmic intermediates. The best studied example is the K+ channel activated by muscarinic agonists in the atrium, which plays a crucial role in regulating the heartbeat. To enable studies of the molecular mechanisms of activation, this channel, denoted KGA, was cloned from a rat atrium cDNA library by functional coupling to coexpressed serotonin type 1A receptors in Xenopus oocytes. KGA displays regions of sequence homology to other inwardly rectifying channels as well as unique regions that may govern G-protein interaction. The expressed KGA channel is activated by serotonin 1A, muscarinic m2, and delta-opioid receptors via G proteins. KGA is activated by guanosine 5'-[gamma-thio]triphosphate in excised patches, confirming activation by a membrane-delimited pathway, and displays a conductance equal to that of the endogenous channel in atrial cells. The hypothesis that similar channels play a role in neuronal inhibition is supported by the cloning of a nearly identical channel (KGB1) from a rat brain cDNA library.
Andrade,
A G protein couples serotonin and GABAB receptors to the same channels in hippocampus.
1986, Pubmed
Andrade,
A G protein couples serotonin and GABAB receptors to the same channels in hippocampus.
1986,
Pubmed
Andrade,
Pharmacologically distinct actions of serotonin on single pyramidal neurones of the rat hippocampus recorded in vitro.
1987,
Pubmed
Bechem,
Removal of Ca current inactivation in dialysed guinea-pig atrial cardioballs by Ca chelators.
1985,
Pubmed
Breitwieser,
Uncoupling of cardiac muscarinic and beta-adrenergic receptors from ion channels by a guanine nucleotide analogue.
,
Pubmed
Brown,
Ionic channels and their regulation by G protein subunits.
1990,
Pubmed
Catterall,
Structure and function of voltage-sensitive ion channels.
1988,
Pubmed
Dascal,
Involvement of a GTP-binding protein in mediation of serotonin and acetylcholine responses in Xenopus oocytes injected with rat brain messenger RNA.
1986,
Pubmed
,
Xenbase
Dascal,
Expression of an atrial G-protein-activated potassium channel in Xenopus oocytes.
1993,
Pubmed
,
Xenbase
EDWARDS,
Changes in membrane characteristics of heart muscle during inhibition.
1956,
Pubmed
Guy,
Pursuing the structure and function of voltage-gated channels.
1990,
Pubmed
,
Xenbase
Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed
Hammer,
Pirenzepine distinguishes between different subclasses of muscarinic receptors.
1980,
Pubmed
Ho,
Cloning and expression of an inwardly rectifying ATP-regulated potassium channel.
1993,
Pubmed
,
Xenbase
Horie,
Rectification of muscarinic K+ current by magnesium ion in guinea pig atrial cells.
1987,
Pubmed
Hoyer,
5-HT receptors: subtypes and second messengers.
1991,
Pubmed
Ito,
On the mechanism of G protein beta gamma subunit activation of the muscarinic K+ channel in guinea pig atrial cell membrane. Comparison with the ATP-sensitive K+ channel.
1992,
Pubmed
Kaibara,
Regulation of spontaneous opening of muscarinic K+ channels in rabbit atrium.
1991,
Pubmed
Karschin,
Heterologously expressed serotonin 1A receptors couple to muscarinic K+ channels in heart.
1991,
Pubmed
Kennelly,
Consensus sequences as substrate specificity determinants for protein kinases and protein phosphatases.
1991,
Pubmed
Kieffer,
The delta-opioid receptor: isolation of a cDNA by expression cloning and pharmacological characterization.
1992,
Pubmed
Kim,
Modulation of acetylcholine-activated K+ channel function in rat atrial cells by phosphorylation.
1991,
Pubmed
Kirsch,
Alpha-subunit of Gk activates atrial K+ channels of chick, rat, and guinea pig.
1988,
Pubmed
Kobilka,
An intronless gene encoding a potential member of the family of receptors coupled to guanine nucleotide regulatory proteins.
,
Pubmed
Kubo,
Primary structure and functional expression of a mouse inward rectifier potassium channel.
1993,
Pubmed
,
Xenbase
Kurachi,
G protein activation of cardiac muscarinic K+ channels.
1992,
Pubmed
Kurachi,
Positive cooperativity in activation of the cardiac muscarinic K+ channel by intracellular GTP.
1990,
Pubmed
Kurachi,
Short-term desensitization of muscarinic K+ channel current in isolated atrial myocytes and possible role of GTP-binding proteins.
1987,
Pubmed
Kyte,
A simple method for displaying the hydropathic character of a protein.
1982,
Pubmed
Logothetis,
The beta gamma subunits of GTP-binding proteins activate the muscarinic K+ channel in heart.
,
Pubmed
Mager,
Steady states, charge movements, and rates for a cloned GABA transporter expressed in Xenopus oocytes.
1993,
Pubmed
,
Xenbase
Mohana Rao,
A conformational preference parameter to predict helices in integral membrane proteins.
1986,
Pubmed
North,
Twelfth Gaddum memorial lecture. Drug receptors and the inhibition of nerve cells.
1989,
Pubmed
Okabe,
The nature and origin of spontaneous noise in G protein-gated ion channels.
1991,
Pubmed
Peralta,
Distinct primary structures, ligand-binding properties and tissue-specific expression of four human muscarinic acetylcholine receptors.
1987,
Pubmed
Pfaffinger,
GTP-binding proteins couple cardiac muscarinic receptors to a K channel.
,
Pubmed
Portoghese,
Naltrindole, a highly selective and potent non-peptide delta opioid receptor antagonist.
1988,
Pubmed
Ruoslahti,
Integrins.
1991,
Pubmed
Sakmann,
Acetylcholine activation of single muscarinic K+ channels in isolated pacemaker cells of the mammalian heart.
,
Pubmed
Yatani,
Direct activation of mammalian atrial muscarinic potassium channels by GTP regulatory protein Gk.
1987,
Pubmed
Yatani,
The G protein-gated atrial K+ channel is stimulated by three distinct Gi alpha-subunits.
1988,
Pubmed
