Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Pflugers Arch
2012 Aug 01;4642:183-91. doi: 10.1007/s00424-012-1125-8.
Show Gene links
Show Anatomy links
Temperature effects on the kinetic properties of the rabbit intestinal oligopeptide cotransporter PepT1.
Bossi E
,
Cherubino F
,
Margheritis E
,
Oyadeyi AS
,
Vollero A
,
Peres A
.
???displayArticle.abstract???
The effects of temperature on the functional properties of the intestinal oligopeptide transporter PepT1 from rabbit have been investigated using electrophysiological methods. The dipeptide Gly-Gln at pH 6.5 or 7.5 was used as substrate. Raising the temperature in the range 20-30 °C causes an increase in the maximal transport-associated current (I (max)) with a Q (10) close to 4. Higher temperatures accelerate the rate of decline of the presteady-state currents observed in the absence of organic substrate. The voltage dependencies of the intramembrane charge movement and of the time constant of decline are both shifted towards more negative potentials by higher temperatures. The shift is due to a stronger action of temperature on the outward rate of charge movement compared to the inward rate, indicating a lower activation energy for the latter process. Consistently, the activation energy for the complete cycle is similar to that of the inward rate of charge movement. Temperature also affects the binding rate of the substrate: the K (0.5) -V curve is shifted to more negative potentials by higher temperatures, resulting in a lower apparent affinity in the physiological range of potentials. The overall efficiency of transport, estimated as the I (max)/K (0.5) ratio is significantly increased at body temperature.
Beckman,
Substrates and temperature differentiate ion flux from serotonin flux in a serotonin transporter.
2001, Pubmed,
Xenbase
Beckman,
Substrates and temperature differentiate ion flux from serotonin flux in a serotonin transporter.
2001,
Pubmed
,
Xenbase
Binda,
Temperature effects on the presteady-state and transport-associated currents of GABA cotransporter rGAT1.
2002,
Pubmed
Bossi,
Residues R282 and D341 act as electrostatic gates in the proton-dependent oligopeptide transporter PepT1.
2011,
Pubmed
,
Xenbase
Cammack,
A GABA transporter operates asymmetrically and with variable stoichiometry.
1994,
Pubmed
De Oliveira,
Differences in the temperature dependence of drug interaction with the noradrenaline and serotonin transporters.
1989,
Pubmed
Eskandari,
Kinetics of the reverse mode of the Na+/glucose cotransporter.
2005,
Pubmed
,
Xenbase
Fields,
Review: Protein function at thermal extremes: balancing stability and flexibility.
2001,
Pubmed
Fields,
Decreases in activation energy and substrate affinity in cold-adapted A4-lactate dehydrogenase: evidence from the Antarctic notothenioid fish Chaenocephalus aceratus.
2004,
Pubmed
Galarza-Muñoz,
Physiological adaptation of an Antarctic Na+/K+-ATPase to the cold.
2011,
Pubmed
,
Xenbase
Giovannardi,
Cl- affects the function of the GABA cotransporter rGAT1 but preserves the mutal relationship between transient and transport currents.
2003,
Pubmed
,
Xenbase
Guastella,
Cloning and expression of a rat brain GABA transporter.
1990,
Pubmed
,
Xenbase
Hazama,
Presteady-state currents of the rabbit Na+/glucose cotransporter (SGLT1).
1997,
Pubmed
,
Xenbase
Hediger,
Expression cloning and cDNA sequencing of the Na+/glucose co-transporter.
,
Pubmed
,
Xenbase
Hilgemann,
GAT1 (GABA:Na+:Cl-) cotransport function. Database reconstruction with an alternating access model.
1999,
Pubmed
,
Xenbase
Kottra,
Inhibition of intracellular dipeptide hydrolysis uncovers large outward transport currents of the peptide transporter PEPT1 in Xenopus oocytes.
2009,
Pubmed
,
Xenbase
Lu,
GAT1 (GABA:Na+:Cl-) cotransport function. Steady state studies in giant Xenopus oocyte membrane patches.
1999,
Pubmed
,
Xenbase
Maffia,
Characterisation of intestinal peptide transporter of the Antarctic haemoglobinless teleost Chionodraco hamatus.
2003,
Pubmed
,
Xenbase
Mertl,
Substrate-induced changes in the density of peptide transporter PEPT1 expressed in Xenopus oocytes.
2008,
Pubmed
,
Xenbase
Nussberger,
Symmetry of H+ binding to the intra- and extracellular side of the H+-coupled oligopeptide cotransporter PepT1.
1997,
Pubmed
,
Xenbase
Renna,
Functional and structural determinants of reverse operation in the pH-dependent oligopeptide transporter PepT1.
2011,
Pubmed
,
Xenbase
Renna,
Unified modeling of the mammalian and fish proton-dependent oligopeptide transporter PepT1.
2011,
Pubmed
,
Xenbase
Sala-Rabanal,
Molecular interactions between dipeptides, drugs and the human intestinal H+ -oligopeptide cotransporter hPEPT1.
2006,
Pubmed
,
Xenbase
Sangaletti,
Functional expression of the oligopeptide transporter PepT1 from the sea bass (Dicentrarchus labrax).
2009,
Pubmed
,
Xenbase
Soragna,
Relations between substrate affinities and charge equilibration rates in the rat GABA cotransporter GAT1.
2005,
Pubmed
,
Xenbase
Wadiche,
Macroscopic and microscopic properties of a cloned glutamate transporter/chloride channel.
1998,
Pubmed
,
Xenbase
Závodszky,
Adjustment of conformational flexibility is a key event in the thermal adaptation of proteins.
1998,
Pubmed
Zeuthen,
Cotransport of water by Na⁺-K⁺-2Cl⁻ cotransporters expressed in Xenopus oocytes: NKCC1 versus NKCC2.
2012,
Pubmed
,
Xenbase
