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Sodium channels from human brain RNA expressed in Xenopus oocytes. Basic electrophysiologic characteristics and their modification by diphenylhydantoin.
Tomaselli GF
,
Marban E
,
Yellen G
.
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We describe the expression and characterization of sodium channels from human brain RNA in the Xenopus oocyte. The expressed channel, studied by whole-cell voltage clamp, reveals characteristic selectivity for sodium as the permeant ion, voltage-dependent gating, and block by nanomolar concentrations of tetrodotoxin. Such channels are not seen in control oocytes injected with solvent only. The anticonvulsant diphenylhydantoin (DPH) inhibits the expressed channel in a voltage- and use-dependent manner, much like the effect seen in primary mammalian neuronal preparations. The inhibition of the expressed human sodium channel by DPH can be described by models previously developed to explain block of Na channels by local anesthetics. The preferential block of Na channels during depolarization helps explain the selectivity of DPH for neurons involved in seizure activity.
Auffray,
Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA.
1980, Pubmed
Auffray,
Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA.
1980,
Pubmed
Auld,
A rat brain Na+ channel alpha subunit with novel gating properties.
1988,
Pubmed
,
Xenbase
Ayala,
The mechanism of action of diphenylhydantoin or invertebrate neurons. I. Effects on basic membrane properties.
1977,
Pubmed
Baud,
Sodium channels induced by depolarization of the Xenopus laevis oocyte.
1982,
Pubmed
,
Xenbase
Baud,
Induction and disappearance of excitability in the oocyte of Xenopus laevis: a voltage-clamp study.
1984,
Pubmed
,
Xenbase
Bean,
Lidocaine block of cardiac sodium channels.
1983,
Pubmed
Bigger,
Effect of lidocaine on the electrophysiological properties of ventricular muscle and purkinje fibers.
1970,
Pubmed
Colatsky,
Voltage clamp measurements of sodium channel properties in rabbit cardiac Purkinje fibres.
1980,
Pubmed
Colatsky,
Electrical properties associated with wide intercellular clefts in rabbit Purkinje fibres.
1979,
Pubmed
Crill,
Neuronal mechanisms of seizure initiation.
1980,
Pubmed
Dascal,
Expression and modulation of voltage-gated calcium channels after RNA injection in Xenopus oocytes.
1986,
Pubmed
,
Xenbase
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Goldin,
Messenger RNA coding for only the alpha subunit of the rat brain Na channel is sufficient for expression of functional channels in Xenopus oocytes.
1986,
Pubmed
,
Xenbase
Gundersen,
Voltage-operated channels induced by foreign messenger RNA in Xenopus oocytes.
1983,
Pubmed
,
Xenbase
Gundersen,
Messenger RNA from human brain induces drug- and voltage-operated channels in Xenopus oocytes.
,
Pubmed
,
Xenbase
Hille,
The permeability of the sodium channel to organic cations in myelinated nerve.
1971,
Pubmed
Hirono,
Characterization of mRNA responsible for induction of functional sodium channels in Xenopus oocytes.
1985,
Pubmed
,
Xenbase
HODGKIN,
A quantitative description of membrane current and its application to conduction and excitation in nerve.
1952,
Pubmed
Hondeghem,
Time- and voltage-dependent interactions of antiarrhythmic drugs with cardiac sodium channels.
1977,
Pubmed
Krafte,
Evidence for the involvement of more than one mRNA species in controlling the inactivation process of rat and rabbit brain Na channels expressed in Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Kusano,
Cholinergic and catecholaminergic receptors in the Xenopus oocyte membrane.
1982,
Pubmed
,
Xenbase
Leonard,
Ca channels induced in Xenopus oocytes by rat brain mRNA.
1987,
Pubmed
,
Xenbase
Lester,
Heterologous expression of excitability proteins: route to more specific drugs?
1988,
Pubmed
Lipicky,
Diphenylhydantoin inhibition of sodium conductance in squid giant axon.
1972,
Pubmed
Mandel,
Selective induction of brain type II Na+ channels by nerve growth factor.
1988,
Pubmed
,
Xenbase
Matsuki,
Characterization of the block of sodium channels by phenytoin in mouse neuroblastoma cells.
1984,
Pubmed
McLean,
Multiple actions of phenytoin on mouse spinal cord neurons in cell culture.
1983,
Pubmed
Methfessel,
Patch clamp measurements on Xenopus laevis oocytes: currents through endogenous channels and implanted acetylcholine receptor and sodium channels.
1986,
Pubmed
,
Xenbase
Neumcke,
Sodium currents and sodium-current fluctuations in rat myelinated nerve fibres.
1982,
Pubmed
Noda,
Expression of functional sodium channels from cloned cDNA.
,
Pubmed
,
Xenbase
Peon,
The relationship of excitability to conduction velocity in canine Purkinje tissue.
1978,
Pubmed
Perry,
The cellular mode of action of the anti-epileptic drug 5,5-diphenylhydantoin.
1978,
Pubmed
Pincus,
Diphenylhydantoin and calcium. Relation to norepinephrine release from brain slices.
1973,
Pubmed
Rosen,
Effects of therapeutic concentrations of diphenylhydantoin on transmembrane potentials of normal and depressed Purkinje fibers.
1976,
Pubmed
Schwarz,
Diphenylhydantoin: excitability reducing action in single myelinated nerve fibres.
1977,
Pubmed
,
Xenbase
Sigel,
Effects of veratridine on single neuronal sodium channels expressed in Xenopus oocytes.
1987,
Pubmed
,
Xenbase
Sigel,
Properties of single sodium channels translated by Xenopus oocytes after injection with messenger ribonucleic acid.
1987,
Pubmed
,
Xenbase
Singh,
Effect of altering potassium concentration on the action of lidocaine and diphenylhydantoin on rabbit atrial and ventricular muscle.
1971,
Pubmed
Starmer,
Mechanisms of use-dependent block of sodium channels in excitable membranes by local anesthetics.
1984,
Pubmed
Stühmer,
Patch clamp characterization of sodium channels expressed from rat brain cDNA.
1987,
Pubmed
,
Xenbase
Sumikawa,
Partial purification and functional expression of brain mRNAs coding for neurotransmitter receptors and voltage-operated channels.
1984,
Pubmed
,
Xenbase
Suzuki,
Functional expression of cloned cDNA encoding sodium channel III.
1988,
Pubmed
,
Xenbase
Twombly,
Mechanisms of calcium channel block by phenytoin.
1988,
Pubmed
Vandenberg,
Inactivation viewed through single sodium channels.
1984,
Pubmed
Willow,
Inhibition of binding of [3H]batrachotoxinin A 20-alpha-benzoate to sodium channels by the anticonvulsant drugs diphenylhydantoin and carbamazepine.
1982,
Pubmed
Willow,
Voltage clamp analysis of the inhibitory actions of diphenylhydantoin and carbamazepine on voltage-sensitive sodium channels in neuroblastoma cells.
1985,
Pubmed
Willow,
Inhibition of voltage-sensitive sodium channels in neuroblastoma cells and synaptosomes by the anticonvulsant drugs diphenylhydantoin and carbamazepine.
1984,
Pubmed
Worley,
Site of anticonvulsant action on sodium channels: autoradiographic and electrophysiological studies in rat brain.
1987,
Pubmed
