Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Cloning of the cDNA for the human beta 1-adrenergic receptor.
Frielle T
,
Collins S
,
Daniel KW
,
Caron MG
,
Lefkowitz RJ
,
Kobilka BK
.
???displayArticle.abstract???
Screening of a human placenta lambda gt11 library has led to the isolation of the cDNA for the human beta 1-adrenergic receptor (beta 1AR). Used as the probe was the human genomic clone termed G-21. This clone, which contains an intronless gene for a putative receptor, was previously isolated by virtue of its cross hybridization with the human beta 2-adrenergic receptor (beta 2AR). The 2.4-kilobase cDNA for the human beta 1AR encodes a protein of 477 amino acid residues that is 69% homologous with the avian beta AR but only 54% homologous with the human beta 2AR. This suggests that the avian gene encoding beta AR and the human gene encoding beta 1AR evolved from a common ancestral gene. RNA blot analysis indicates a message of 2.5 kilobases in rat tissues, with a pattern of tissue distribution consistent with beta 1AR binding. This pattern is quite distinct from the pattern obtained when the beta 2AR cDNA is used as a probe. Expression of receptor protein in Xenopus laevis oocytes conveys adenylate cyclase responsiveness to catecholamines with a typical beta 1AR specificity. This contrasts with the typical beta 2 subtype specificity observed when the human beta 2AR cDNA is expressed in this system. Mammalian beta 1AR and beta 2AR are thus products of distinct genes, both of which are apparently related to the putative G-21 receptor.
Aziz,
Both subunits of rat liver ferritin are regulated at a translational level by iron induction.
1986, Pubmed
Aziz,
Both subunits of rat liver ferritin are regulated at a translational level by iron induction.
1986,
Pubmed
Benovic,
Beta-adrenergic receptor kinase: identification of a novel protein kinase that phosphorylates the agonist-occupied form of the receptor.
1986,
Pubmed
Benton,
Screening lambdagt recombinant clones by hybridization to single plaques in situ.
1977,
Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Chirgwin,
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
1979,
Pubmed
Dixon,
Cloning of the gene and cDNA for mammalian beta-adrenergic receptor and homology with rhodopsin.
,
Pubmed
Dohlman,
A family of receptors coupled to guanine nucleotide regulatory proteins.
1987,
Pubmed
Feinberg,
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
1983,
Pubmed
Gilman,
G proteins and dual control of adenylate cyclase.
1984,
Pubmed
Kobilka,
cDNA for the human beta 2-adrenergic receptor: a protein with multiple membrane-spanning domains and encoded by a gene whose chromosomal location is shared with that of the receptor for platelet-derived growth factor.
1987,
Pubmed
Kobilka,
An intronless gene encoding a potential member of the family of receptors coupled to guanine nucleotide regulatory proteins.
,
Pubmed
Konarska,
Recognition of cap structure in splicing in vitro of mRNA precursors.
1984,
Pubmed
Kozak,
Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles.
1983,
Pubmed
Kubo,
Cloning, sequencing and expression of complementary DNA encoding the muscarinic acetylcholine receptor.
,
Pubmed
,
Xenbase
Kubo,
Primary structure of porcine cardiac muscarinic acetylcholine receptor deduced from the cDNA sequence.
1986,
Pubmed
Kühn,
Light-induced binding of guanosinetriphosphatase to bovine photoreceptor membranes: effect of limited proteolysis of the membranes.
1981,
Pubmed
Kyte,
A simple method for displaying the hydropathic character of a protein.
1982,
Pubmed
Lefkowitz,
Stimulation of catecholamine-sensitive adenylate cyclase by 5'-guanylyl-imidodiphosphate.
1974,
Pubmed
Maniatis,
Nucleotide sequence of the rightward operator of phage lambda.
1975,
Pubmed
McMaster,
Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.
1977,
Pubmed
Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed
Minneman,
A comparison of the beta-adrenergic receptor of the turkey erythrocyte with mammalian beta1 and beta2 receptors.
1980,
Pubmed
Nathans,
Molecular genetics of human color vision: the genes encoding blue, green, and red pigments.
1986,
Pubmed
Peralta,
Primary structure and biochemical properties of an M2 muscarinic receptor.
1987,
Pubmed
Salomon,
A highly sensitive adenylate cyclase assay.
1974,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Schocken,
The human placenta--a rich source of beta-adrenergic receptors: characterization of the receptors in particulate and solubilized preparations.
1980,
Pubmed
Stiles,
Beta-adrenergic receptors: biochemical mechanisms of physiological regulation.
1984,
Pubmed
,
Xenbase
Vieira,
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
1982,
Pubmed
Yarden,
The avian beta-adrenergic receptor: primary structure and membrane topology.
1986,
Pubmed
