XB-ART-27916
FASEB J
1987 Nov 01;15:380-7. doi: 10.1096/fasebj.1.5.2824269.
Show Gene links
Show Anatomy links
Expression of rat mRNA coding for hormone-stimulated adenylate cyclase in Xenopus oocytes.
???displayArticle.abstract???
Beta-Adrenergic agonist-stimulated hyperpolarization, whole-cell cAMP accumulation, and activity of isoproterenol-stimulated membrane-bound adenylate cyclase (EC 4.6.1.1) in Xenopus laevis ovarian oocytes are entirely dependent on the presence of nascent follicle cells. A method was developed to remove rapidly and completely all extra-oocyte cell types to yield defolliculated oocytes that exhibited normal viability and resting membrane potentials yet lacked beta-adrenergic receptor (beta AR)-stimulated responses. Purified follicle membranes contained beta AR-stimulated adenylate cyclase activity, whereas oocyte cell membranes did not. Purified oocyte membrane preparations from X. laevis oocytes previously microinjected with C6-2B rat astrocytoma mRNA, and subsequently defolliculated, exhibited novel beta AR and forskolin-stimulated adenylate cyclase activity. These experiments demonstrate that oocytes expressed rat C6-2B mRNA coding for the beta-adrenergic receptor and the components necessary for forskolin-stimulated adenylate cyclase activity.
???displayArticle.pubmedLink??? 2824269
???displayArticle.link??? FASEB J
???displayArticle.grants???
Genes referenced: camp