Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Eur Biophys J
1987 Jan 01;143:131-8. doi: 10.1007/bf00253837.
Show Gene links
Show Anatomy links
Patch clamp characterization of sodium channels expressed from rat brain cDNA.
Stühmer W
,
Methfessel C
,
Sakmann B
,
Noda M
,
Numa S
.
???displayArticle.abstract???
Sodium currents, INa, were recorded from Xenopus laevis oocytes which had been injected with mRNA synthesized by in vitro transcription of the rat brain sodium channel II cDNA (Noda et al. 1986 a, b). Patch pipettes were used to apply depolarizing voltage steps and to record macroscopic sodium currents of between 50 and 750 pA from cell-attached patches of the oocyte membrane. With a combination of whole-cell and patch clamp recording, the properties of the implanted sodium channels could be studied in detail. They were analyzed according to the model of Hodgkin and Huxley (1952 a) assuming three activation gates. The activation of the sodium currents is characterized by an equilibrium potential of -29 mV and an apparent gating charge of 8.7 e0. At -64 mV half of the sodium currents were inactivated. From single-channel current recordings, an elementary sodium channel conductance of 19 pS and an average open time of 0.43 ms were obtained at -32 mV membrane potential and 16 degrees C. The single-channel and activation properties of rat brain sodium channel II are therefore comparable to those found in peripheral nerve and skeletal muscle, but inactivation occurs at less negative potentials. This could be a specific property of the brain sodium channels and may underlie the maintained inward sodium currents reported in brain neurones (French and Gage 1985).
Almers,
Voltage clamp of rat and human skeletal muscle: measurements with an improved loose-patch technique.
1984, Pubmed
Almers,
Voltage clamp of rat and human skeletal muscle: measurements with an improved loose-patch technique.
1984,
Pubmed
Baud,
Sodium channels induced by depolarization of the Xenopus laevis oocyte.
1982,
Pubmed
,
Xenbase
FRANKENHAEUSER,
Quantitative description of sodium currents in myelinated nerve fibres of Xenopus laevis.
1960,
Pubmed
,
Xenbase
French,
A threshold sodium current in pyramidal cells in rat hippocampus.
1985,
Pubmed
Green,
Human beta-globin pre-mRNA synthesized in vitro is accurately spliced in Xenopus oocyte nuclei.
1983,
Pubmed
,
Xenbase
Gundersen,
Voltage-operated channels induced by foreign messenger RNA in Xenopus oocytes.
1983,
Pubmed
,
Xenbase
Gundersen,
Messenger RNA from human brain induces drug- and voltage-operated channels in Xenopus oocytes.
,
Pubmed
,
Xenbase
Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed
Hirono,
Characterization of mRNA responsible for induction of functional sodium channels in Xenopus oocytes.
1985,
Pubmed
,
Xenbase
HODGKIN,
The dual effect of membrane potential on sodium conductance in the giant axon of Loligo.
1952,
Pubmed
HODGKIN,
A quantitative description of membrane current and its application to conduction and excitation in nerve.
1952,
Pubmed
Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed
Methfessel,
Patch clamp measurements on Xenopus laevis oocytes: currents through endogenous channels and implanted acetylcholine receptor and sodium channels.
1986,
Pubmed
,
Xenbase
Mishina,
Molecular distinction between fetal and adult forms of muscle acetylcholine receptor.
,
Pubmed
,
Xenbase
Neumcke,
Sodium currents and sodium-current fluctuations in rat myelinated nerve fibres.
1982,
Pubmed
Neumcke,
Slow actions of hyperpolarization on sodium channels in the membrane of myelinated nerve.
1979,
Pubmed
Noda,
Expression of functional sodium channels from cloned cDNA.
,
Pubmed
,
Xenbase
Noda,
Primary structure of Electrophorus electricus sodium channel deduced from cDNA sequence.
,
Pubmed
Noda,
Existence of distinct sodium channel messenger RNAs in rat brain.
,
Pubmed
Pappone,
Voltage-clamp experiments in normal and denervated mammalian skeletal muscle fibres.
1980,
Pubmed
Ruff,
Comparison between slow sodium channel inactivation in rat slow- and fast-twitch muscle.
1987,
Pubmed
Sakmann,
Role of acetylcholine receptor subunits in gating of the channel.
,
Pubmed
,
Xenbase
Sigworth,
Single Na+ channel currents observed in cultured rat muscle cells.
1980,
Pubmed
Sigworth,
The variance of sodium current fluctuations at the node of Ranvier.
1980,
Pubmed
Simoncini,
Slow sodium channel inactivation in rat fast-twitch muscle.
1987,
Pubmed
Sumikawa,
Partial purification and functional expression of brain mRNAs coding for neurotransmitter receptors and voltage-operated channels.
1984,
Pubmed
,
Xenbase
