Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Considerable evidence suggests that the Homer family of scaffolding proteins contributes to synaptic organization and function. We investigated the role of both Homer 1b, the constitutively expressed, and developmentally regulated form of Homer, and Homer 1a, the activity-induced immediate early gene, in dendritic arbor elaboration and synaptic function of developing Xenopus optic tectal neurons. We expressed exogenous Homer 1a or Homer 1b in developing Xenopus tectal neurons. By collecting in vivo time lapse images of individual, EGFP-labeled and Homer-expressing neurons over 3 days, we found that Homer 1b leads to a significant decrease in dendritic arbor growth rate and arbor size. Synaptic transmission was also altered in developing neurons transfected with Homer 1b. Cells expressing exogenous Homer 1b over 3 days had a significantly greater AMPA to NMDA ratios, and increased AMPA mEPSC frequency. These data suggest that increasing Homer 1b expression increases excitatory synaptic inputs, increases synaptic maturation, and slows dendritic arbor growth rate. Exogenous Homer 1a expression increases AMPA mEPSC frequency, but did not significantly affect tectal cell dendritic arbor development. Changes in the ratio of Homer 1a to Homer 1b may signal the neuron that overall activity levels in the cell have changed, and this in turn could affect protein interactions at the synapse, synaptic transmission, and structural development of the dendritic arbor.
Aizenman,
Visually driven regulation of intrinsic neuronal excitability improves stimulus detection in vivo.
2003, Pubmed,
Xenbase
Aizenman,
Visually driven regulation of intrinsic neuronal excitability improves stimulus detection in vivo.
2003,
Pubmed
,
Xenbase
Aizenman,
Visually driven modulation of glutamatergic synaptic transmission is mediated by the regulation of intracellular polyamines.
2002,
Pubmed
,
Xenbase
Ango,
Agonist-independent activation of metabotropic glutamate receptors by the intracellular protein Homer.
2001,
Pubmed
Ango,
Dendritic and axonal targeting of type 5 metabotropic glutamate receptor is regulated by homer1 proteins and neuronal excitation.
2000,
Pubmed
Beneken,
Structure of the Homer EVH1 domain-peptide complex reveals a new twist in polyproline recognition.
2000,
Pubmed
Bestman,
In vivo single-cell electroporation for transfer of DNA and macromolecules.
2006,
Pubmed
,
Xenbase
Bottai,
Synaptic activity-induced conversion of intronic to exonic sequence in Homer 1 immediate early gene expression.
2002,
Pubmed
Brakeman,
Homer: a protein that selectively binds metabotropic glutamate receptors.
1997,
Pubmed
Cantallops,
Postsynaptic CPG15 promotes synaptic maturation and presynaptic axon arbor elaboration in vivo.
2000,
Pubmed
,
Xenbase
Celikel,
Select overexpression of homer1a in dorsal hippocampus impairs spatial working memory.
2007,
Pubmed
Chen,
Regulation of dendritic development by neuronal activity.
2005,
Pubmed
Ciruela,
Homer-1c/Vesl-1L modulates the cell surface targeting of metabotropic glutamate receptor type 1alpha: evidence for an anchoring function.
2000,
Pubmed
Ciruela,
Co-expression of metabotropic glutamate receptor type 1alpha with homer-1a/Vesl-1S increases the cell surface expression of the receptor.
1999,
Pubmed
Cline,
Dendritic arbor development and synaptogenesis.
2001,
Pubmed
Cline,
The regulation of dendritic arbor development and plasticity by glutamatergic synaptic input: a review of the synaptotrophic hypothesis.
2008,
Pubmed
Goel,
Persistence of experience-induced homeostatic synaptic plasticity through adulthood in superficial layers of mouse visual cortex.
2007,
Pubmed
Haas,
AMPA receptors regulate experience-dependent dendritic arbor growth in vivo.
2006,
Pubmed
,
Xenbase
Haas,
Single-cell electroporation for gene transfer in vivo.
2001,
Pubmed
,
Xenbase
Haas,
Targeted electroporation in Xenopus tadpoles in vivo--from single cells to the entire brain.
2002,
Pubmed
,
Xenbase
Hennou,
Homer-1a/Vesl-1S enhances hippocampal synaptic transmission.
2003,
Pubmed
Inoue,
Homer1a regulates the activity-induced remodeling of synaptic structures in cultured hippocampal neurons.
2007,
Pubmed
Kammermeier,
Homer 1a uncouples metabotropic glutamate receptor 5 from postsynaptic effectors.
2007,
Pubmed
Kato,
vesl, a gene encoding VASP/Ena family related protein, is upregulated during seizure, long-term potentiation and synaptogenesis.
1997,
Pubmed
Maffei,
Selective reconfiguration of layer 4 visual cortical circuitry by visual deprivation.
2004,
Pubmed
Matsuzaki,
Structural basis of long-term potentiation in single dendritic spines.
2004,
Pubmed
Rajan,
Glutamate receptor activity is required for normal development of tectal cell dendrites in vivo.
1998,
Pubmed
,
Xenbase
Rajan,
NMDA receptor activity stabilizes presynaptic retinotectal axons and postsynaptic optic tectal cell dendrites in vivo.
1999,
Pubmed
,
Xenbase
Ronesi,
Homer interactions are necessary for metabotropic glutamate receptor-induced long-term depression and translational activation.
2008,
Pubmed
Sala,
Regulation of dendritic spine morphology and synaptic function by Shank and Homer.
2001,
Pubmed
Sala,
Inhibition of dendritic spine morphogenesis and synaptic transmission by activity-inducible protein Homer1a.
2003,
Pubmed
Shinoda,
Repetition of mGluR-dependent LTD causes slowly developing persistent reduction in synaptic strength accompanied by synapse elimination.
2005,
Pubmed
Tadokoro,
Involvement of unique leucine-zipper motif of PSD-Zip45 (Homer 1c/vesl-1L) in group 1 metabotropic glutamate receptor clustering.
1999,
Pubmed
Tu,
Homer binds a novel proline-rich motif and links group 1 metabotropic glutamate receptors with IP3 receptors.
1998,
Pubmed
Tu,
Coupling of mGluR/Homer and PSD-95 complexes by the Shank family of postsynaptic density proteins.
1999,
Pubmed
Turrigiano,
Homeostatic plasticity in the developing nervous system.
2004,
Pubmed
Turrigiano,
Thinking globally, acting locally: AMPA receptor turnover and synaptic strength.
1998,
Pubmed
Van Keuren-Jensen,
Visual experience regulates metabotropic glutamate receptor-mediated plasticity of AMPA receptor synaptic transmission by homer1a induction.
2006,
Pubmed
,
Xenbase
Wu,
Stabilization of dendritic arbor structure in vivo by CaMKII.
1998,
Pubmed
,
Xenbase
Wu,
Maturation of a central glutamatergic synapse.
1996,
Pubmed
,
Xenbase
Xiao,
Homer regulates the association of group 1 metabotropic glutamate receptors with multivalent complexes of homer-related, synaptic proteins.
1998,
Pubmed
,
Xenbase
Yuan,
Homer binds TRPC family channels and is required for gating of TRPC1 by IP3 receptors.
2003,
Pubmed
Zhou,
Shrinkage of dendritic spines associated with long-term depression of hippocampal synapses.
2004,
Pubmed
Zhu,
Acute versus chronic NMDA receptor blockade and synaptic AMPA receptor delivery.
2002,
Pubmed
Zou,
Postsynaptic calcium/calmodulin-dependent protein kinase II is required to limit elaboration of presynaptic and postsynaptic neuronal arbors.
1999,
Pubmed
,
Xenbase
Zucker,
Short-term synaptic plasticity.
2002,
Pubmed
