Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
The kinase Aurora-A (Aur-A), which is enriched at centrosomes, is required for centrosome maturation and accurate chromosome segregation, and recent work implicates centrosomes as sites where the earliest activation of cyclin B1-cdc2 occurs. Here, we have used Xenopus egg extracts to investigate Aur-A's contribution to cell cycle progression and spindle morphology in the presence or absence of centrosomes. We find that addition of active Aur-A accelerates cdc2 activation and mitotic entry. Depletion of endogenous Aur-A or addition of inactive Aur-A, which lead to monopolar spindles, delays but does not block mitotic entry. These effects on timing and spindle structure do not require the presence of centrosomes or chromosomes. The catalytic domain alone of Aur-A is sufficient to restore spindle bipolarity; additional N-terminal sequences function in mitotic timing.
Anderson,
Ionizing radiation induces apoptosis and elevates cyclin A1-Cdk2 activity before but not after the midblastula transition in Xenopus.
1997, Pubmed,
Xenbase
Anderson,
Ionizing radiation induces apoptosis and elevates cyclin A1-Cdk2 activity before but not after the midblastula transition in Xenopus.
1997,
Pubmed
,
Xenbase
Andrésson,
The kinase Eg2 is a component of the Xenopus oocyte progesterone-activated signaling pathway.
1998,
Pubmed
,
Xenbase
Bayliss,
Structural basis of Aurora-A activation by TPX2 at the mitotic spindle.
2003,
Pubmed
,
Xenbase
Bitangcol,
Activation of the p42 mitogen-activated protein kinase pathway inhibits Cdc2 activation and entry into M-phase in cycling Xenopus egg extracts.
1998,
Pubmed
,
Xenbase
Blagden,
Polar expeditions--provisioning the centrosome for mitosis.
2003,
Pubmed
Brittle,
Centrosome maturation: Aurora lights the way to the poles.
2005,
Pubmed
Chau,
Mos-induced p42 mitogen-activated protein kinase activation stabilizes M-phase in Xenopus egg extracts after cyclin destruction.
1998,
Pubmed
,
Xenbase
Dasso,
Completion of DNA replication is monitored by a feedback system that controls the initiation of mitosis in vitro: studies in Xenopus.
1990,
Pubmed
,
Xenbase
De Souza,
Centrosomal and cytoplasmic Cdc2/cyclin B1 activation precedes nuclear mitotic events.
2000,
Pubmed
Du,
Suppression of p160ROCK bypasses cell cycle arrest after Aurora-A/STK15 depletion.
2004,
Pubmed
Ducat,
Aurora kinases in spindle assembly and chromosome segregation.
2004,
Pubmed
Duckworth,
G2 arrest in Xenopus oocytes depends on phosphorylation of cdc25 by protein kinase A.
2002,
Pubmed
,
Xenbase
Dutertre,
Phosphorylation of CDC25B by Aurora-A at the centrosome contributes to the G2-M transition.
2004,
Pubmed
,
Xenbase
Eyers,
A novel mechanism for activation of the protein kinase Aurora A.
2003,
Pubmed
,
Xenbase
Gabrielli,
Cytoplasmic accumulation of cdc25B phosphatase in mitosis triggers centrosomal microtubule nucleation in HeLa cells.
1996,
Pubmed
Gadde,
Mechanisms and molecules of the mitotic spindle.
2004,
Pubmed
Gadea,
Aurora kinase inhibitor ZM447439 blocks chromosome-induced spindle assembly, the completion of chromosome condensation, and the establishment of the spindle integrity checkpoint in Xenopus egg extracts.
2005,
Pubmed
,
Xenbase
Goyal,
Ajuba, a novel LIM protein, interacts with Grb2, augments mitogen-activated protein kinase activity in fibroblasts, and promotes meiotic maturation of Xenopus oocytes in a Grb2- and Ras-dependent manner.
1999,
Pubmed
,
Xenbase
Groisman,
Translational control of embryonic cell division by CPEB and maskin.
2001,
Pubmed
,
Xenbase
Guadagno,
Requirement for MAPK activation for normal mitotic progression in Xenopus egg extracts.
1998,
Pubmed
,
Xenbase
Hannak,
Aurora-A kinase is required for centrosome maturation in Caenorhabditis elegans.
2001,
Pubmed
Henikoff,
Centromeric chromatin: what makes it unique?
2005,
Pubmed
Hirota,
Aurora-A and an interacting activator, the LIM protein Ajuba, are required for mitotic commitment in human cells.
2003,
Pubmed
,
Xenbase
Hodgman,
CPEB phosphorylation and cytoplasmic polyadenylation are catalyzed by the kinase IAK1/Eg2 in maturing mouse oocytes.
2001,
Pubmed
,
Xenbase
Jackman,
Active cyclin B1-Cdk1 first appears on centrosomes in prophase.
2003,
Pubmed
Karlsson,
Cdc25B and Cdc25C differ markedly in their properties as initiators of mitosis.
1999,
Pubmed
Kumagai,
14-3-3 proteins act as negative regulators of the mitotic inducer Cdc25 in Xenopus egg extracts.
1998,
Pubmed
,
Xenbase
Kunitoku,
CENP-A phosphorylation by Aurora-A in prophase is required for enrichment of Aurora-B at inner centromeres and for kinetochore function.
2003,
Pubmed
Lammer,
The cdc25B phosphatase is essential for the G2/M phase transition in human cells.
1998,
Pubmed
Littlepage,
Identification of a new APC/C recognition domain, the A box, which is required for the Cdh1-dependent destruction of the kinase Aurora-A during mitotic exit.
2002,
Pubmed
,
Xenbase
Ma,
Biphasic activation of Aurora-A kinase during the meiosis I- meiosis II transition in Xenopus oocytes.
2003,
Pubmed
,
Xenbase
Marumoto,
Roles of aurora-A kinase in mitotic entry and G2 checkpoint in mammalian cells.
2002,
Pubmed
,
Xenbase
Mendez,
Phosphorylation of CPEB by Eg2 mediates the recruitment of CPSF into an active cytoplasmic polyadenylation complex.
2000,
Pubmed
,
Xenbase
Mendez,
Phosphorylation of CPE binding factor by Eg2 regulates translation of c-mos mRNA.
2000,
Pubmed
,
Xenbase
Meraldi,
Aurora kinases link chromosome segregation and cell division to cancer susceptibility.
2004,
Pubmed
Minshull,
A MAP kinase-dependent spindle assembly checkpoint in Xenopus egg extracts.
1994,
Pubmed
,
Xenbase
Murray,
Cell cycle extracts.
1991,
Pubmed
Roghi,
The Xenopus protein kinase pEg2 associates with the centrosome in a cell cycle-dependent manner, binds to the spindle microtubules and is involved in bipolar mitotic spindle assembly.
1998,
Pubmed
,
Xenbase
Sagata,
What does Mos do in oocytes and somatic cells?
1997,
Pubmed
,
Xenbase
Sasayama,
Over-expression of Aurora-A targets cytoplasmic polyadenylation element binding protein and promotes mRNA polyadenylation of Cdk1 and cyclin B1.
2005,
Pubmed
,
Xenbase
Schmitt,
Signalling pathways in oocyte meiotic maturation.
2002,
Pubmed
,
Xenbase
Stanford,
Changes in regulatory phosphorylation of Cdc25C Ser287 and Wee1 Ser549 during normal cell cycle progression and checkpoint arrests.
2005,
Pubmed
,
Xenbase
Tay,
The control of cyclin B1 mRNA translation during mouse oocyte maturation.
2000,
Pubmed
Tsai,
A Ran signalling pathway mediated by the mitotic kinase Aurora A in spindle assembly.
2003,
Pubmed
,
Xenbase
Varmark,
Functional role of centrosomes in spindle assembly and organization.
2004,
Pubmed
Walter,
Induction of a G2-phase arrest in Xenopus egg extracts by activation of p42 mitogen-activated protein kinase.
1997,
Pubmed
,
Xenbase
