Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Chromosoma
1997 Jun 01;1057-8:506-14. doi: 10.1007/bf02510487.
Show Gene links
Show Anatomy links
Delocalization of some small nucleolar RNPs after actinomycin D treatment to deplete early pre-rRNAs.
Rivera-León R
,
Gerbi SA
.
???displayArticle.abstract???
Retention of some components within the nucleolus correlates with the presence of rRNA precursors found early in the rRNA processing pathway. Specifically, after most 40S, 38S and 36S pre-rRNAs have been depleted by incubation of Xenopus kidney cells in 0.05 microg/ml actinomycin D for 4 h, only 69% U3 small nucleolar RNA (snoRNA), 68% U14 snoRNA and 72% fibrillarin are retained in the nucleolus as compared with control cells. These nucleolar components are important for processing steps in the pathway that gives rise to 18S rRNA. In contrast, U8 snoRNA, which is used for 5.8S and 28S rRNA production, is fully retained in the nucleolus after actinomycin D treatment. Therefore, U8 snoRNA is in a different category than U3 and U14 snoRNA and fibrillarin. It is proposed that U3 and U14 snoRNA and fibrillarin, but not U8 snoRNA, bind to the external transcribed spacer or internal transcribed spacer 1, and when these binding sites are lost after actinomycin D treatment some of these components cannot be retained in the nucleolus. Other binding sites may also exist, which would explain why only some and not all of these components are lost from the nucleolus.
Azum-Gélade,
Cell cycle redistribution of U3 snRNA and fibrillarin. Presence in the cytoplasmic nucleolus remnant and in the prenucleolar bodies at telophase.
1994, Pubmed
Azum-Gélade,
Cell cycle redistribution of U3 snRNA and fibrillarin. Presence in the cytoplasmic nucleolus remnant and in the prenucleolar bodies at telophase.
1994,
Pubmed
Bachellerie,
Recognition signals for mouse pre-rRNA processing. A potential role for U3 nucleolar RNA.
1983,
Pubmed
Baserga,
An intact Box C sequence in the U3 snRNA is required for binding of fibrillarin, the protein common to the major family of nucleolar snRNPs.
1991,
Pubmed
Beltrame,
Base pairing between U3 and the pre-ribosomal RNA is required for 18S rRNA synthesis.
1995,
Pubmed
Beltrame,
Identification and functional analysis of two U3 binding sites on yeast pre-ribosomal RNA.
1992,
Pubmed
,
Xenbase
Beltrame,
Mutational analysis of an essential binding site for the U3 snoRNA in the 5' external transcribed spacer of yeast pre-rRNA.
1994,
Pubmed
Carmo-Fonseca,
In vivo detection of snRNP-rich organelles in the nuclei of mammalian cells.
1991,
Pubmed
Cavaillé,
Targeted ribose methylation of RNA in vivo directed by tailored antisense RNA guides.
1996,
Pubmed
Chu,
The RNA of RNase MRP is required for normal processing of ribosomal RNA.
1994,
Pubmed
Epstein,
Multiple states of U3 RNA in Novikoff hepatoma nucleoli.
1984,
Pubmed
Escande-Géraud,
Correlation between rDNA transcription and distribution of a 100 kD nucleolar protein in CHO cells.
1985,
Pubmed
Fischer,
Assigning functions to nucleolar structures.
1991,
Pubmed
Fournier,
The nucleolar snRNAs: catching up with the spliceosomal snRNAs.
1993,
Pubmed
Gerbi,
Small nucleolar RNA.
1995,
Pubmed
Glibetic,
Regulation of U3 snRNA expression during myoblast differentiation.
1992,
Pubmed
Henríquez,
Isolation and sequencing of NOP1. A yeast gene encoding a nucleolar protein homologous to a human autoimmune antigen.
1990,
Pubmed
Henry,
The 5' end of yeast 5.8S rRNA is generated by exonucleases from an upstream cleavage site.
1994,
Pubmed
Hernandez-Verdun,
Autoimmune serum containing an antibody against a 94 kDa nucleolar protein.
1988,
Pubmed
Hughes,
Depletion of U3 small nucleolar RNA inhibits cleavage in the 5' external transcribed spacer of yeast pre-ribosomal RNA and impairs formation of 18S ribosomal RNA.
1991,
Pubmed
Hughes,
Functional base-pairing interaction between highly conserved elements of U3 small nucleolar RNA and the small ribosomal subunit RNA.
1996,
Pubmed
Jarmolowski,
Identification of essential elements in U14 RNA of Saccharomyces cerevisiae.
1990,
Pubmed
Jeppesen,
Nucleotide sequence determination and secondary structure of Xenopus U3 snRNA.
1988,
Pubmed
,
Xenbase
Jiménez-García,
Nucleologenesis: U3 snRNA-containing prenucleolar bodies move to sites of active pre-rRNA transcription after mitosis.
1994,
Pubmed
Kass,
The U3 small nucleolar ribonucleoprotein functions in the first step of preribosomal RNA processing.
1990,
Pubmed
Kiss-László,
Site-specific ribose methylation of preribosomal RNA: a novel function for small nucleolar RNAs.
1996,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lapeyre,
Molecular cloning of Xenopus fibrillarin, a conserved U3 small nuclear ribonucleoprotein recognized by antisera from humans with autoimmune disease.
1990,
Pubmed
,
Xenbase
Li,
Depletion of U14 small nuclear RNA (snR128) disrupts production of 18S rRNA in Saccharomyces cerevisiae.
1990,
Pubmed
Lischwe,
Purification and partial characterization of a nucleolar scleroderma antigen (Mr = 34,000; pI, 8.5) rich in NG,NG-dimethylarginine.
1985,
Pubmed
Loreni,
Translational regulation of ribosomal protein synthesis in Xenopus cultured cells: mRNA relocation between polysomes and RNP during nutritional shifts.
1992,
Pubmed
,
Xenbase
Maser,
U3 small nuclear RNA can be psoralen-cross-linked in vivo to the 5' external transcribed spacer of pre-ribosomal-RNA.
1989,
Pubmed
Matera,
Organization of small nucleolar ribonucleoproteins (snoRNPs) by fluorescence in situ hybridization and immunocytochemistry.
1994,
Pubmed
Maxwell,
The small nucleolar RNAs.
1995,
Pubmed
Miller,
Visualization of nucleolar genes.
1969,
Pubmed
Morrissey,
Yeast snR30 is a small nucleolar RNA required for 18S rRNA synthesis.
1993,
Pubmed
Mougey,
The terminal balls characteristic of eukaryotic rRNA transcription units in chromatin spreads are rRNA processing complexes.
1993,
Pubmed
,
Xenbase
Nicoloso,
Intron-encoded, antisense small nucleolar RNAs: the characterization of nine novel species points to their direct role as guides for the 2'-O-ribose methylation of rRNAs.
1996,
Pubmed
Ochs,
Fibrillarin: a new protein of the nucleolus identified by autoimmune sera.
1985,
Pubmed
,
Xenbase
Peculis,
Sequence and structural elements critical for U8 snRNP function in Xenopus oocytes are evolutionarily conserved.
1994,
Pubmed
,
Xenbase
Peculis,
Disruption of U8 nucleolar snRNA inhibits 5.8S and 28S rRNA processing in the Xenopus oocyte.
1993,
Pubmed
,
Xenbase
Perry,
THE CELLULAR SITES OF SYNTHESIS OF RIBOSOMAL AND 4S RNA.
1962,
Pubmed
Perry,
Persistent synthesis of 5S RNA when production of 28S and 18S ribosomal RNA is inhibited by low doses of actinomycin D.
1968,
Pubmed
Perry,
Inhibition of RNA synthesis by actinomycin D: characteristic dose-response of different RNA species.
1970,
Pubmed
Prestayko,
Low molecular weight RNA associated with 28 s nucleolar RNA.
1970,
Pubmed
Puvion-Dutilleul,
Localization of U3 RNA molecules in nucleoli of HeLa and mouse 3T3 cells by high resolution in situ hybridization.
1991,
Pubmed
Puvion-Dutilleul,
Alterations of nucleolar ultrastructure and ribosome biogenesis by actinomycin D. Implications for U3 snRNP function.
1992,
Pubmed
Raska,
Immunological and ultrastructural studies of the nuclear coiled body with autoimmune antibodies.
1991,
Pubmed
Reimer,
Human autoantibodies: probes for nucleolus structure and function.
1987,
Pubmed
Reimer,
Monoclonal autoantibody from a (New Zealand black x New Zealand white)F1 mouse and some human scleroderma sera target an Mr 34,000 nucleolar protein of the U3 RNP particle.
1987,
Pubmed
Savino,
In vivo disruption of Xenopus U3 snRNA affects ribosomal RNA processing.
1990,
Pubmed
,
Xenbase
Scheer,
Functional and dynamic aspects of the mammalian nucleolus.
1990,
Pubmed
Schimmang,
A yeast nucleolar protein related to mammalian fibrillarin is associated with small nucleolar RNA and is essential for viability.
1989,
Pubmed
Schmitt,
Nuclear RNase MRP is required for correct processing of pre-5.8S rRNA in Saccharomyces cerevisiae.
1993,
Pubmed
Seiler-Tuyns,
The human estrogen receptor can regulate exogenous but not endogenous vitellogenin gene promoters in a Xenopus cell line.
1988,
Pubmed
,
Xenbase
Shanab,
Determination of the nucleotide sequences in mouse U14 small nuclear RNA and 18S ribosomal RNA responsible for in vitro intermolecular base-pairing.
1992,
Pubmed
Sienna,
Altered subcellular distribution of U3 snRNA in response to serum in mouse fibroblasts.
1996,
Pubmed
Spector,
Silver staining, immunofluorescence, and immunoelectron microscopic localization of nucleolar phosphoproteins B23 and C23.
1984,
Pubmed
Stroke,
The 5' end of U3 snRNA can be crosslinked in vivo to the external transcribed spacer of rat ribosomal RNA precursors.
1989,
Pubmed
Tague,
Processing of the large rRNA precursor: two proposed categories of RNA-RNA interactions in eukaryotes.
1984,
Pubmed
Tollervey,
Temperature-sensitive mutations demonstrate roles for yeast fibrillarin in pre-rRNA processing, pre-rRNA methylation, and ribosome assembly.
1993,
Pubmed
Tollervey,
A yeast small nuclear RNA is required for normal processing of pre-ribosomal RNA.
1987,
Pubmed
Tollervey,
The small nucleolar RNP protein NOP1 (fibrillarin) is required for pre-rRNA processing in yeast.
1991,
Pubmed
Tyc,
A new interaction between the mouse 5' external transcribed spacer of pre-rRNA and U3 snRNA detected by psoralen crosslinking.
1992,
Pubmed
Tyc,
U3, U8 and U13 comprise a new class of mammalian snRNPs localized in the cell nucleolus.
1989,
Pubmed
Tycowski,
Requirement for intron-encoded U22 small nucleolar RNA in 18S ribosomal RNA maturation.
1994,
Pubmed
,
Xenbase
Wu,
Snurposomes and coiled bodies.
1993,
Pubmed
,
Xenbase
Xia,
Intronic U14 snoRNAs of Xenopus laevis are located in two different parent genes and can be processed from their introns during early oogenesis.
1995,
Pubmed
,
Xenbase
Yung,
Effects of actinomycin D analogs on nucleolar phosphoprotein B23 (37,000 daltons/pI 5.1).
1985,
Pubmed
Yung,
Translocation of nucleolar phosphoprotein B23 (37 kDa/pI 5.1) induced by selective inhibitors of ribosome synthesis.
1985,
Pubmed
Yung,
Effects of luzopeptins on protein B23 translocation and ribosomal RNA synthesis in HeLa cells.
1986,
Pubmed
Zagorski,
Characterization of an SNR gene locus in Saccharomyces cerevisiae that specifies both dispensible and essential small nuclear RNAs.
1988,
Pubmed
Zieve,
Small RNA species of the HeLa cell: metabolism and subcellular localization.
1976,
Pubmed
