XB-ART-20153
Brain Res Mol Brain Res
1995 Feb 01;282:343-8. doi: 10.1016/0169-328x(94)00244-9.
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Cloning and expression of a bovine glutamate transporter.
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We have isolated a 3845 base-pair cDNA (BNGLUAS) encoding a bovine glutamate transporter (bovine GLAST) by screening a bovine retina cDNA library with an oligonucleotide probe corresponding to conserved regions of known glutamate transporters. The cDNA sequence predicted a protein of 542 amino acids and displayed 96% and 97% amino acid identity with the rat GLAST/GluT-1 and human GLAST, respectively. Expression of the bovine GLAST in Xenopus oocytes revealed Na(+)-dependent [14C]L-glutamate uptake and electrogenic glutamate uptake.
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Species referenced: Xenopus
Genes referenced: slc1a3