Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Cell Physiol
2011 Nov 01;22611:2740-6. doi: 10.1002/jcp.22854.
Show Gene links
Show Anatomy links
Voltage sensitive phosphoinositide phosphatases of Xenopus: their tissue distribution and voltage dependence.
Ratzan WJ
,
Evsikov AV
,
Okamura Y
,
Jaffe LA
.
???displayArticle.abstract???
Voltage-sensitive phosphatases (VSPs) are unique proteins in which membrane potential controls enzyme activity. They are comprised of the voltage sensor domain of an ion channel coupled to a lipid phosphatase specific for phosphoinositides, and for ascidian and zebrafish VSPs, the phosphatase activity has been found to be activated by membrane depolarization. The physiological functions of these proteins are unknown, but their expression in testis and embryos suggests a role in fertilization or development. Here we investigate the expression pattern and voltage dependence of VSPs in two frog species, Xenopus laevis and Xenopus tropicalis, that are well suited for experimental studies of these possible functions. X. laevis has two VSP genes (Xl-VSP1 and Xl-VSP2), whereas X. tropicalis has only one gene (Xt-VSP). The highest expression of these genes was observed in testis, ovary, liver, and kidney. Our results show that while Xl-VSP2 activates only at positive membrane potentials outside of the physiological range, Xl-VSP1 and Xt-VSP phosphatase activity is regulated in the voltage range that regulates sperm-egg fusion at fertilization.
Barrero-Villar,
PI4P5-kinase Ialpha is required for efficient HIV-1 entry and infection of T cells.
2008, Pubmed
Barrero-Villar,
PI4P5-kinase Ialpha is required for efficient HIV-1 entry and infection of T cells.
2008,
Pubmed
Bezanilla,
How membrane proteins sense voltage.
2008,
Pubmed
Chen,
A testis-specific gene, TPTE, encodes a putative transmembrane tyrosine phosphatase and maps to the pericentromeric region of human chromosomes 21 and 13, and to chromosomes 15, 22, and Y.
1999,
Pubmed
Dimitrov,
Engineering and characterization of an enhanced fluorescent protein voltage sensor.
2007,
Pubmed
Duesbery,
The role of Ca(2+) in progesterone-induced germinal vesicle breakdown of Xenopus laevis oocytes: the synergic effects of microtubule depolymerization and Ca(2+).
1996,
Pubmed
,
Xenbase
Evsikov,
Gene expression during the oocyte-to-embryo transition in mammals.
2009,
Pubmed
Falkenburger,
Kinetics of PIP2 metabolism and KCNQ2/3 channel regulation studied with a voltage-sensitive phosphatase in living cells.
2010,
Pubmed
Glahn,
Voltage-clamp study of the activation currents and fast block to polyspermy in the egg of Xenopus laevis.
2003,
Pubmed
,
Xenbase
Goudeau,
Evidence by a voltage clamp study of an electrically mediated block to polyspermy in the egg of the ascidian Phallusia mammillata.
1994,
Pubmed
Grey,
An electrical block is required to prevent polyspermy in eggs fertilized by natural mating of Xenopus laevis.
1982,
Pubmed
,
Xenbase
Halaszovich,
Ci-VSP is a depolarization-activated phosphatidylinositol-4,5-bisphosphate and phosphatidylinositol-3,4,5-trisphosphate 5'-phosphatase.
2009,
Pubmed
Hossain,
Enzyme domain affects the movement of the voltage sensor in ascidian and zebrafish voltage-sensing phosphatases.
2008,
Pubmed
,
Xenbase
Huang,
CAP3: A DNA sequence assembly program.
1999,
Pubmed
Iwao,
Activation of Xenopus Eggs by an Extract of Cynops Sperm: (fertilization/egg-activation/polyspermy block/sperm protease).
1994,
Pubmed
,
Xenbase
Iwao,
Evidence that the voltage-dependent component in the fertilization process is contributed by the sperm.
1989,
Pubmed
Iwasaki,
A voltage-sensing phosphatase, Ci-VSP, which shares sequence identity with PTEN, dephosphorylates phosphatidylinositol 4,5-bisphosphate.
2008,
Pubmed
,
Xenbase
Jaffe,
Studies of the voltage-dependent polyspermy block using cross-species fertilization of amphibians.
1983,
Pubmed
,
Xenbase
Jaffe,
Fast block to polyspermy in sea urchin eggs is electrically mediated.
1976,
Pubmed
Jaffe,
Studies of the mechanism of the electrical polyspermy block using voltage clamp during cross-species fertilization.
1982,
Pubmed
Kado,
The rise and fall of electrical excitability in the oocyte of Xenopus laevis.
1981,
Pubmed
,
Xenbase
Kline,
A calcium-activated sodium conductance contributes to the fertilization potential in the egg of the nemertean worm Cerebratulus lacteus.
1986,
Pubmed
Kohout,
Subunit organization and functional transitions in Ci-VSP.
2008,
Pubmed
Murata,
Depolarization activates the phosphoinositide phosphatase Ci-VSP, as detected in Xenopus oocytes coexpressing sensors of PIP2.
2007,
Pubmed
,
Xenbase
Murata,
Phosphoinositide phosphatase activity coupled to an intrinsic voltage sensor.
2005,
Pubmed
,
Xenbase
Neuhaus,
Kidney specific expression of cTPTE during development of the chick embryo.
2009,
Pubmed
Ogasawara,
Gene expression profile of Ci-VSP in juveniles and adult blood cells of ascidian.
2011,
Pubmed
Okamura,
Voltage-sensing phosphatase: its molecular relationship with PTEN.
2011,
Pubmed
Okamura,
Biodiversity of voltage sensor domain proteins.
2007,
Pubmed
Reymond,
Human chromosome 21 gene expression atlas in the mouse.
2002,
Pubmed
Richter,
CPEB: a life in translation.
2007,
Pubmed
,
Xenbase
Stauffer,
Receptor-induced transient reduction in plasma membrane PtdIns(4,5)P2 concentration monitored in living cells.
1998,
Pubmed
Tamura,
MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0.
2007,
Pubmed
Tapparel,
The TPTE gene family: cellular expression, subcellular localization and alternative splicing.
2003,
Pubmed
Terasaki,
Changes in organization of the endoplasmic reticulum during Xenopus oocyte maturation and activation.
2001,
Pubmed
,
Xenbase
Várnai,
Visualization of phosphoinositides that bind pleckstrin homology domains: calcium- and agonist-induced dynamic changes and relationship to myo-[3H]inositol-labeled phosphoinositide pools.
1998,
Pubmed
Villalba-Galea,
Coupling between the voltage-sensing and phosphatase domains of Ci-VSP.
2009,
Pubmed
,
Xenbase
Walker,
TPIP: a novel phosphoinositide 3-phosphatase.
2001,
Pubmed
Wu,
PTEN 2, a Golgi-associated testis-specific homologue of the PTEN tumor suppressor lipid phosphatase.
2001,
Pubmed
Zhang,
Mechanosensitivity of GIRK channels is mediated by protein kinase C-dependent channel-phosphatidylinositol 4,5-bisphosphate interaction.
2004,
Pubmed
,
Xenbase
