Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Voltage-dependent sodium channel function is regulated through membrane mechanics.
Shcherbatko A
,
Ono F
,
Mandel G
,
Brehm P
.
???displayArticle.abstract???
Cut-open recordings from Xenopus oocytes expressing either nerve (PN1) or skeletal muscle (SkM1) Na(+) channel alpha subunits revealed slow inactivation onset and recovery kinetics of inward current. In contrast, recordings using the macropatch configuration resulted in an immediate negative shift in the voltage-dependence of inactivation and activation, as well as time-dependent shifts in kinetics when compared to cut-open recordings. Specifically, a slow transition from predominantly slow onset and recovery to exclusively fast onset and fast recovery from inactivation occurred. The shift to fast inactivation was accelerated by patch excision and by agents that disrupted microtubule formation. Application of positive pressure to cell-attached macropatch electrodes prevented the shift in kinetics, while negative pressure led to an abrupt shift to fast inactivation. Simultaneous electrophysiological recording and video imaging of the cell-attached patch membrane revealed that the pressure-induced shift to fast inactivation coincided with rupture of sites of membrane attachment to cytoskeleton. These findings raise the possibility that the negative shift in voltage-dependence and the fast kinetics observed normally for endogenous Na(+) channels involve mechanical destabilization. Our observation that the beta1 subunit causes similar changes in function of the Na(+) channel alpha subunit suggests that beta1 may act through interaction with cytoskeleton.
Auld,
A rat brain Na+ channel alpha subunit with novel gating properties.
1988, Pubmed,
Xenbase
Auld,
A rat brain Na+ channel alpha subunit with novel gating properties.
1988,
Pubmed
,
Xenbase
Cachelin,
Sodium channels in cultured cardiac cells.
1983,
Pubmed
Chahine,
Functional expression and properties of the human skeletal muscle sodium channel.
1994,
Pubmed
,
Xenbase
Chen,
Modulation of Na+ channel inactivation by the beta 1 subunit: a deletion analysis.
1995,
Pubmed
,
Xenbase
Cooper,
Effects of cytochalasin and phalloidin on actin.
1987,
Pubmed
Fahlke,
Giga-seal formation alters properties of sodium channels of human myoballs.
1992,
Pubmed
Featherstone,
Interaction between fast and slow inactivation in Skm1 sodium channels.
1996,
Pubmed
,
Xenbase
Fenwick,
Sodium and calcium channels in bovine chromaffin cells.
1982,
Pubmed
Fernandez,
Membrane patches and whole-cell membranes: a comparison of electrical properties in rat clonal pituitary (GH3) cells.
1984,
Pubmed
Fleig,
Kinetic mode switch of rat brain IIA Na channels in Xenopus oocytes excised macropatches.
1994,
Pubmed
,
Xenbase
Fukuda,
Breakdown of cytoskeletal filaments selectively reduces Na and Ca spikes in cultured mammal neurones.
1981,
Pubmed
Gee,
Interaction of muscle and brain sodium channels with multiple members of the syntrophin family of dystrophin-associated proteins.
1998,
Pubmed
Goldin,
Messenger RNA coding for only the alpha subunit of the rat brain Na channel is sufficient for expression of functional channels in Xenopus oocytes.
1986,
Pubmed
,
Xenbase
Hamill,
Induced membrane hypo/hyper-mechanosensitivity: a limitation of patch-clamp recording.
1997,
Pubmed
Hartshorne,
The sodium channel from rat brain. Purification and subunit composition.
1984,
Pubmed
Haydon,
Some effects of n-pentane on the sodium and potassium currents of the squid giant axon.
1981,
Pubmed
Hendry,
Further evidence that membrane thickness influences voltage-gated sodium channels.
1985,
Pubmed
Hoebeke,
Interaction of oncodazole (R 17934), a new antitumoral drug, with rat brain tubulin.
1976,
Pubmed
Isom,
Auxiliary subunits of voltage-gated ion channels.
1994,
Pubmed
Isom,
Primary structure and functional expression of the beta 1 subunit of the rat brain sodium channel.
1992,
Pubmed
,
Xenbase
Joho,
Toxin and kinetic profile of rat brain type III sodium channels expressed in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Kimitsuki,
Negative shift of cardiac Na+ channel kinetics in cell-attached patch recordings.
1990,
Pubmed
Klugbauer,
Structure and functional expression of a new member of the tetrodotoxin-sensitive voltage-activated sodium channel family from human neuroendocrine cells.
1995,
Pubmed
Kordeli,
An isoform of ankyrin is localized at nodes of Ranvier in myelinated axons of central and peripheral nerves.
1990,
Pubmed
Krafte,
Inactivation of cloned Na channels expressed in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Kunze,
Cardiac Na currents and the inactivating, reopening, and waiting properties of single cardiac Na channels.
1985,
Pubmed
Kwiatkowski,
Functions of gelsolin: motility, signaling, apoptosis, cancer.
1999,
Pubmed
Lundbaek,
Membrane stiffness and channel function.
1996,
Pubmed
Makita,
Voltage-gated Na+ channel beta 1 subunit mRNA expressed in adult human skeletal muscle, heart, and brain is encoded by a single gene.
1994,
Pubmed
,
Xenbase
Matsumoto,
Axonal microtubules necessary for generation of sodium current in squid giant axons: I. Pharmacological study on sodium current and restoration of sodium current by microtubule proteins and 260K protein.
1984,
Pubmed
Matsumoto,
Axonal microtubules necessary for generation of sodium current in squid giant axons: II. Effect of colchicine upon asymmetrical displacement current.
1984,
Pubmed
Milton,
Na current in membrane blebs: implications for channel mobility and patch clamp recording.
1990,
Pubmed
Moorman,
Fast and slow gating of sodium channels encoded by a single mRNA.
1990,
Pubmed
,
Xenbase
Murray,
A single site on the epsilon subunit is responsible for the change in ACh receptor channel conductance during skeletal muscle development.
1995,
Pubmed
,
Xenbase
Opsahl,
Transduction of membrane tension by the ion channel alamethicin.
1994,
Pubmed
Qu,
Modulation of cardiac Na+ channels expressed in a mammalian cell line and in ventricular myocytes by protein kinase C.
1994,
Pubmed
Rudy,
Slow inactivation of the sodium conductance in squid giant axons. Pronase resistance.
1978,
Pubmed
Scheuer,
Functional properties of rat brain sodium channels expressed in a somatic cell line.
1990,
Pubmed
Sokabe,
The structure and dynamics of patch-clamped membranes: a study using differential interference contrast light microscopy.
1990,
Pubmed
Srinivasan,
Ankyrin and spectrin associate with voltage-dependent sodium channels in brain.
1988,
Pubmed
Taglialatela,
Novel voltage clamp to record small, fast currents from ion channels expressed in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Townsend,
Effect of alkali metal cations on slow inactivation of cardiac Na+ channels.
1997,
Pubmed
,
Xenbase
Trimmer,
Primary structure and functional expression of a mammalian skeletal muscle sodium channel.
1989,
Pubmed
,
Xenbase
Ukomadu,
muI Na+ channels expressed transiently in human embryonic kidney cells: biochemical and biophysical properties.
1992,
Pubmed
Vedantham,
Slow inactivation does not affect movement of the fast inactivation gate in voltage-gated Na+ channels.
1998,
Pubmed
,
Xenbase
Wendt,
Na channel kinetics remain stable during perforated-patch recordings.
1992,
Pubmed
West,
Efficient expression of rat brain type IIA Na+ channel alpha subunits in a somatic cell line.
1992,
Pubmed
Zhou,
Multiple gating modes and the effect of modulating factors on the microI sodium channel.
1991,
Pubmed
,
Xenbase
