XB-ART-3321
Biochem Biophys Res Commun
2004 Jul 30;3203:680-4. doi: 10.1016/j.bbrc.2004.06.017.
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Transcriptional regulation of the TGF-beta pseudoreceptor BAMBI by TGF-beta signaling.
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BAMBI is a transmembrane glycoprotein related to the transforming growth factor-beta (TGF-beta)-family type I receptors and functions as a negative regulator of TGF-beta signaling during development. BAMBI is induced by BMP signaling through the evolutionary conserved BMP-responsive elements in its promoter. Furthermore, we have recently shown that Wnt/beta-catenin signaling activates transcription of BAMBI and that BAMBI expression is aberrantly elevated in most colorectal carcinomas. Here, we show that BAMBI is also directly induced by TGF-beta signaling, through the three tandem repeats of 13 bp sequences containing the SMAD-binding elements, which are distinct from the BMP-responsive element. Our findings suggest that BAMBI transcription is regulated by TGF-beta signaling through direct binding of SMAD3 and SMAD4 to the BAMBI promoter.
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Species referenced: Xenopus
Genes referenced: bambi smad10 smad3 smad4 tgfb1