Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
The sequence of 300 nucleotides surrounding the transcription termination site for ribosomal RNA in Drosophila melanogaster has been determined. The precise position of the RNA 3'-end was located by sizing rDNA fragments protected from S1 nuclease by hybridization with cytoplasmic 28S rRNA or with nuclear 38S pre-rRNA. Next, the sequence of the first 10 nucleotides at the 3'-end of 28S and 38S rRNA was determined directly. The results indicate the absence of 3'-terminal processing of the 38S pre-rRNA. The nucleotide sequence from a position 27 nucleotides upstream to 150 nucleotides downstream from the termination site was identical in three uninterrupted rRNA genes and one interrupted gene, except for a single base change in the spacer region of one clone. The terminal 100 nucleotides of Drosophila 28S rRNA have more than 60 per cent homology with the corresponding region of Xenopus 28S rRNA and yeast 26S rRNA.
Adhya,
Control of transcription termination.
1978, Pubmed
Adhya,
Control of transcription termination.
1978,
Pubmed
Biswas,
Eukaryotic RNA polymerases and the factors that control them.
1975,
Pubmed
Brown,
Synthesis and accumulation of DNA-like RNA during embryogenesis of Xenopus laevis.
1966,
Pubmed
,
Xenbase
Dawid,
Ribosomal DNA in Drosophila melanogaster. I. Isolation and characterization of cloned fragments.
1978,
Pubmed
Donis-Keller,
Mapping adenines, guanines, and pyrimidines in RNA.
1977,
Pubmed
England,
Specific labeling of 3' termini of RNA with T4 RNA ligase.
1980,
Pubmed
Gurdon,
The influence of the cytoplasm on the nucleus during cell differentiation, with special reference to RNA synthesis during amphibian cleavage.
1969,
Pubmed
Hamada,
3'-terminal processing of ribosomal RNA precursors in mammalian cells.
1980,
Pubmed
Jolly,
Nuclear RNA transcripts from Drosophila melanogaster ribosomal RNA genes containing introns.
1980,
Pubmed
Korn,
Nucleotide sequence of Xenopus borealis oocyte 5S DNA: comparison of sequences that flank several related eucaryotic genes.
1978,
Pubmed
,
Xenbase
Levis,
Processing steps and methylation in the formation of the ribosomal RNA of cultured Drosophila cells.
1978,
Pubmed
Long,
Repeated genes in eukaryotes.
1980,
Pubmed
,
Xenbase
Long,
Expression of ribosomal DNA insertions in Drosophila melanogaster.
1979,
Pubmed
Long,
Alternative pathways in the processing of ribosomal RNA precursor in Drosophila melanogaster.
1980,
Pubmed
Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed
Pavlakis,
Sequence and secondary structure of Drosophila melanogaster 5.8S and 2S rRNAs and of the processing site between them.
1979,
Pubmed
Rae,
The 10 kb Drosophila virilis 28S rDNA intervening sequence is flanked by a direct repeat of 14 base pairs of coding sequence.
1980,
Pubmed
,
Xenbase
Sinclair,
Retention of common nucleotide sequences in the ribosomal deoxyribonucleic acid of eukaryotes and some of their physical characteristics.
1971,
Pubmed
Sollner-Webb,
The nucleotide sequence of the initiation and termination sites for ribosomal RNA transcription in X. laevis.
1979,
Pubmed
,
Xenbase
Tabak,
A method for the recovery of DNA from agarose gels.
1978,
Pubmed
Urano,
The nucleotide sequence of the putative transcription initiation site of a cloned ribosomal RNA gene of the mouse.
1980,
Pubmed
,
Xenbase
Veldman,
The transcription termination site of the ribosomal RNA operon in yeast.
1980,
Pubmed
,
Xenbase
Wellauer,
Secondary structure maps of ribosomal RNA. II. Processing of mouse L-cell ribosomal RNA and variations in the processing pathway.
1974,
Pubmed
Wieslander,
A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels.
1979,
Pubmed
