Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
The nucleotide sequence of the putative transcription initiation site of a cloned ribosomal RNA gene of the mouse.
Urano Y
,
Kominami R
,
Mishima Y
,
Muramatsu M
.
???displayArticle.abstract???
Approximately one kilobase pairs surrounding and upstream the transcription initiation site of a cloned ribosomal DNA (rDNA) of the mouse were sequenced. The putative transcription initiation site was determined by two independent methods: one nuclease S1 protection and the other reverse transcriptase elongation mapping using isolated 45S ribosomal RNA precursor (45S RNA) and appropriate restriction fragments of rDNA. Both methods gave an identical result; 45S RNA had a structure starting from ACTCTTAG---. Characteristically, mouse rDNA had many T clusters (greater than or equal to 5) upstream the initiation site, the longest being 21 consecutive T's. A pentadecanucleotide, TGCCTCCCGAGTGCA, appeared twice within 260 nucleotides upstream the putative initiation site. No such characteristic sequences were found downstream this site. Little similarity was found in the upstream of the transcription initiation site between the mouse, Xenopus laevis and Saccharomyces cerevisiae rDNA.
Arnheim,
Characterization of mouse ribosomal gene fragments purified by molecular cloning.
1979, Pubmed
Arnheim,
Characterization of mouse ribosomal gene fragments purified by molecular cloning.
1979,
Pubmed
Batts-Young,
Triphosphate residues at the 5' ends of rRNA precursor and 5S RNA from Dictyostelium discoideum.
1978,
Pubmed
Baulieu,
Some aspects of the mechanism of action of steroid hormones.
1975,
Pubmed
Berk,
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
1977,
Pubmed
Bina-Stein,
Rapid sequence determination of late simian virus 40 16S mRNA leader by using inhibitors of reverse transcriptase.
1979,
Pubmed
Birnstiel,
Properties and composition of the isolated ribosomal DNA satellite of Xenopus laevis.
1968,
Pubmed
,
Xenbase
Boseley,
Sequence organization of the spacer DNA in a ribosomal gene unit of Xenopus laevis.
1979,
Pubmed
,
Xenbase
Brown,
Gene linkage by RNA-DNA hybridization. II. Arrangement of the redundant gene sequences for 28 s and 18 s ribosomal RNA.
1968,
Pubmed
,
Xenbase
Brown,
Synthesis and accumulation of DNA-like RNA during embryogenesis of Xenopus laevis.
1966,
Pubmed
,
Xenbase
BROWN,
RNA SYNTHESIS DURING THE DEVELOPMENT OF XENOPUS LAEVIS, THE SOUTH AFRICAN CLAWED TOAD.
1964,
Pubmed
,
Xenbase
Chambon,
Eukaryotic nuclear RNA polymerases.
1975,
Pubmed
,
Xenbase
Currier,
Isolation of covalently closed circular DNA of high molecular weight from bacteria.
1976,
Pubmed
Grummt,
Characterization of a cloned ribosomal fragment from mouse which contains the 18S coding region and adjacent spacer sequences.
1979,
Pubmed
Gurdon,
The influence of the cytoplasm on the nucleus during cell differentiation, with special reference to RNA synthesis during amphibian cleavage.
1969,
Pubmed
Hayashi,
Effect of cycloheximide on the synthesis and processing of 5S RNA in HeLa cells.
1977,
Pubmed
Higashi,
Selective suppression of nucleolar RNA metabolism in the absence of protein synthesis.
1968,
Pubmed
HOLLAND,
EFFECTS OF PUROMYCIN ON RNA SYNTHESIS IN MAMMALIAN CELLS.
1963,
Pubmed
Klemenz,
The 5' terminus of the precursor ribosomal RNA of Saccharomyces cerevisiae.
1980,
Pubmed
Klootwijk,
The primary transcript of the ribosomal repeating unit in yeast.
1979,
Pubmed
Kominami,
Heterogeneity of 5' -termini of nucleolar 45S, 32S and 28S RNA in mouse hepatoma.
1977,
Pubmed
Konkel,
The evolution and sequence comparison of two recently diverged mouse chromosomal beta--globin genes.
1979,
Pubmed
Konkel,
The sequence of the chromosomal mouse beta-globin major gene: homologies in capping, splicing and poly(A) sites.
1978,
Pubmed
Matsui,
Nucleolar DNA-dependent RNA polymerase from rat liver. 1. Purification and subunit structure.
1976,
Pubmed
Maxam,
A new method for sequencing DNA.
1977,
Pubmed
Mishima,
Cloning and determination of a putative promoter region of a mouse ribosomal deoxyribonucleic acid fragment.
1980,
Pubmed
Mishima,
The mechanism of decrease in nucleolar RNA synthesis by protein synthesis inhibition.
1979,
Pubmed
Miyahara,
Differential inhibition of 28S-18S RNA and 5S RNA synthesis in the cycloheximide-treated Xenopus laevis embryonic cells.
1970,
Pubmed
,
Xenbase
Moss,
The putative promoter of a Xenopus laevis ribosomal gene is reduplicated.
1979,
Pubmed
,
Xenbase
Muramatsu,
Effect of cycloheximide on the nucleolar RNA synthesis in rat liver.
1970,
Pubmed
Niles,
Isolation of a high specific activity 35S ribosomal RNA precursor from Tetrahymena pyriformis and identification of its 5' terminus, pppAp.
1978,
Pubmed
Nishioka,
The complete sequence of a chromosomal mouse alpha--globin gene reveals elements conserved throughout vertebrate evolution.
1979,
Pubmed
Reeder,
Sites of transcription initiation in vivo on Xenopus laevis ribosomal DNA.
1977,
Pubmed
,
Xenbase
Schwartz,
Purification and subunit structure of deoxyribonucleic acid-dependent ribonucleic acid polymerase I from the mouse myeloma, MOPC 315.
1974,
Pubmed
Smith,
A simple method for DNA restriction site mapping.
1976,
Pubmed
Sollner-Webb,
The nucleotide sequence of the initiation and termination sites for ribosomal RNA transcription in X. laevis.
1979,
Pubmed
,
Xenbase
Tabak,
A method for the recovery of DNA from agarose gels.
1978,
Pubmed
Thomas,
Hybridization of RNA to double-stranded DNA: formation of R-loops.
1976,
Pubmed
Tonegawa,
Sequence of a mouse germ-line gene for a variable region of an immunoglobulin light chain.
1978,
Pubmed
Valenzuela,
Ribosomal RNA genes of Saccharomyces cerevisiae. II. Physical map and nucleotide sequence of the 5 S ribosomal RNA gene and adjacent intergenic regions.
1977,
Pubmed
Wellauer,
Secondary structure maps of ribosomal RNA and DNA. I. Processing of Xenopus laevis ribosomal RNA and structure of single-stranded ribosomal DNA.
1974,
Pubmed
,
Xenbase
Willems,
The regulation of RNA synthesis and processing in the nucleolus during inhibition of protein synthesis.
1969,
Pubmed
Ziff,
Coincidence of the promoter and capped 5' terminus of RNA from the adenovirus 2 major late transcription unit.
1978,
Pubmed
