Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Ser-3 is important for regulating Mos interaction with and stimulation of mitogen-activated protein kinase kinase.
Chen M
,
Cooper JA
.
???displayArticle.abstract??? Mos is a germ cell-specific serine/threonine protein kinase that activates mitogen-activated protein kinase (MAPK) through MAPK kinase (MKK). In Xenopus oocytes, Mos synthesis is required for progesterone-induced activation of MAPK and maturation promoting factor. Injection of Mos or active MAPK causes mitotic arrest in early embryos, suggesting that Mos also acts via MKK and MAPK to induce the arrest of unfertilized eggs in metaphase of meiosis II. We have investigated whether Mos activity is regulated by phosphorylation. Previous studies have identified Ser-3 as the principal autophosphorylation site. We show that Mos interacts with the catalytic domain of MKK in a Saccharomyces cerevisiae two-hybrid test. Acidic substitutions of the sites phosphorylated by Mos in MKK reduce the interaction, implying that the complex may dissociate after phosphorylation of MKK by Mos. Furthermore, the Mos-MKK interaction requires Mos kinase activity, suggesting that Mos autophosphorylation may be involved in the interaction. Substitution of Ser-3 of Mos with Ala reduces the interaction with MKK and also reduces both the activation of MKK by Mos in vitro and cleavage arrest induced by Mos fusion protein in Xenopus embryos. By contrast, substitution of Ser-3 by Glu, an acidic amino acid that mimics phosphoserine, fosters the Mos interaction with MKK and permits activation of MKK in vitro and Mos-induced cleavage arrest. Moreover, the Glu-3 substitution increases the interaction of a kinase-inactive Mos mutant with MKK. Taken together, these results suggest that an important step in Mos activation involves the phosphorylation at Ser-3, which promotes Mos interaction with and activation of MKK.
Alessi,
Identification of the sites in MAP kinase kinase-1 phosphorylated by p74raf-1.
1994, Pubmed
Alessi,
Identification of the sites in MAP kinase kinase-1 phosphorylated by p74raf-1.
1994,
Pubmed
Bai,
Phosphorylation of v-mos Ser 47 by the mitotic form of p34cdc2.
1991,
Pubmed
Barrett,
Ha-rasVal-12,Thr-59 activates S6 kinase and p34cdc2 kinase in Xenopus oocytes: evidence for c-mosxe-dependent and -independent pathways.
1990,
Pubmed
,
Xenbase
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Cobb,
Extracellular signal-regulated kinases: ERKs in progress.
1991,
Pubmed
Colledge,
Disruption of c-mos causes parthenogenetic development of unfertilized mouse eggs.
1994,
Pubmed
,
Xenbase
Cooper,
MAP kinase pathways. Straight and narrow or tortuous and intersecting?
1994,
Pubmed
Cowley,
Activation of MAP kinase kinase is necessary and sufficient for PC12 differentiation and for transformation of NIH 3T3 cells.
1994,
Pubmed
Daar,
A characterization of cytostatic factor activity from Xenopus eggs and c-mos-transformed cells.
1991,
Pubmed
,
Xenbase
Davis,
The mitogen-activated protein kinase signal transduction pathway.
1993,
Pubmed
Deshpande,
Insulin induction of Xenopus laevis oocyte maturation is inhibited by monoclonal antibody against p21 ras proteins.
1987,
Pubmed
,
Xenbase
Fabian,
A single amino acid change in Raf-1 inhibits Ras binding and alters Raf-1 function.
1994,
Pubmed
,
Xenbase
Fabian,
Requirement for Raf and MAP kinase function during the meiotic maturation of Xenopus oocytes.
1993,
Pubmed
,
Xenbase
Ferrell,
Cell cycle tyrosine phosphorylation of p34cdc2 and a microtubule-associated protein kinase homolog in Xenopus oocytes and eggs.
1991,
Pubmed
,
Xenbase
Fields,
The two-hybrid system: an assay for protein-protein interactions.
1994,
Pubmed
Freeman,
Phosphorylation of conserved serine residues does not regulate the ability of mosxe protein kinase to induce oocyte maturation or function as cytostatic factor.
1992,
Pubmed
,
Xenbase
Fukasawa,
Similarities between somatic cells overexpressing the mos oncogene and oocytes during meiotic interphase.
1994,
Pubmed
,
Xenbase
Furuno,
Suppression of DNA replication via Mos function during meiotic divisions in Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Gotoh,
In vitro effects on microtubule dynamics of purified Xenopus M phase-activated MAP kinase.
1991,
Pubmed
,
Xenbase
Gotoh,
Characterization of recombinant Xenopus MAP kinase kinases mutated at potential phosphorylation sites.
1994,
Pubmed
,
Xenbase
Haccard,
Induction of metaphase arrest in cleaving Xenopus embryos by MAP kinase.
1993,
Pubmed
,
Xenbase
Hanks,
The protein kinase family: conserved features and deduced phylogeny of the catalytic domains.
1988,
Pubmed
Hashimoto,
Parthenogenetic activation of oocytes in c-mos-deficient mice.
1994,
Pubmed
,
Xenbase
Hattori,
Activation of mitogen-activated protein kinase and its activator by ras in intact cells and in a cell-free system.
1992,
Pubmed
,
Xenbase
Huang,
Raf-1 forms a stable complex with Mek1 and activates Mek1 by serine phosphorylation.
1993,
Pubmed
Kanki,
Progression from meiosis I to meiosis II in Xenopus oocytes requires de novo translation of the mosxe protooncogene.
1991,
Pubmed
,
Xenbase
Kirschner,
The cell cycle then and now.
1992,
Pubmed
Kosako,
Requirement for the MAP kinase kinase/MAP kinase cascade in Xenopus oocyte maturation.
1994,
Pubmed
,
Xenbase
Lange-Carter,
A divergence in the MAP kinase regulatory network defined by MEK kinase and Raf.
1993,
Pubmed
Lorca,
Calmodulin-dependent protein kinase II mediates inactivation of MPF and CSF upon fertilization of Xenopus eggs.
1993,
Pubmed
,
Xenbase
Maller,
Xenopus oocytes and the biochemistry of cell division.
1990,
Pubmed
,
Xenbase
Mansour,
Transformation of mammalian cells by constitutively active MAP kinase kinase.
1994,
Pubmed
Marshall,
MAP kinase kinase kinase, MAP kinase kinase and MAP kinase.
1994,
Pubmed
Maxwell,
Serine kinase activity associated with Maloney murine sarcoma virus-124-encoded p37mos.
1985,
Pubmed
Minshull,
Cyclin synthesis: who needs it?
1993,
Pubmed
Moodie,
Complexes of Ras.GTP with Raf-1 and mitogen-activated protein kinase kinase.
1993,
Pubmed
Muslin,
Raf-1 protein kinase is important for progesterone-induced Xenopus oocyte maturation and acts downstream of mos.
1993,
Pubmed
,
Xenbase
Nebreda,
The c-mos proto-oncogene protein kinase turns on and maintains the activity of MAP kinase, but not MPF, in cell-free extracts of Xenopus oocytes and eggs.
1993,
Pubmed
,
Xenbase
Nishizawa,
The 'second-codon rule' and autophosphorylation govern the stability and activity of Mos during the meiotic cell cycle in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Paules,
Mouse Mos protooncogene product is present and functions during oogenesis.
1989,
Pubmed
,
Xenbase
Pelech,
MAP kinases: charting the regulatory pathways.
1992,
Pubmed
Pomerance,
Stimulation of mitogen-activated protein kinase by oncogenic Ras p21 in Xenopus oocytes. Requirement for Ras p21-GTPase-activating protein interaction.
1992,
Pubmed
,
Xenbase
Posada,
Mos stimulates MAP kinase in Xenopus oocytes and activates a MAP kinase kinase in vitro.
1993,
Pubmed
,
Xenbase
Posada,
Requirements for phosphorylation of MAP kinase during meiosis in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Posada,
Tyrosine phosphorylation and activation of homologous protein kinases during oocyte maturation and mitogenic activation of fibroblasts.
1991,
Pubmed
,
Xenbase
Resing,
Determination of v-Mos-catalyzed phosphorylation sites and autophosphorylation sites on MAP kinase kinase by ESI/MS.
1995,
Pubmed
Robbins,
Regulation and properties of extracellular signal-regulated protein kinases 1 and 2 in vitro.
1993,
Pubmed
Sagata,
Function of c-mos proto-oncogene product in meiotic maturation in Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Sagata,
The product of the mos proto-oncogene as a candidate "initiator" for oocyte maturation.
1989,
Pubmed
,
Xenbase
Sagata,
The c-mos proto-oncogene product is a cytostatic factor responsible for meiotic arrest in vertebrate eggs.
1989,
Pubmed
,
Xenbase
Seger,
Overexpression of mitogen-activated protein kinase kinase (MAPKK) and its mutants in NIH 3T3 cells. Evidence that MAPKK involvement in cellular proliferation is regulated by phosphorylation of serine residues in its kinase subdomains VII and VIII.
1994,
Pubmed
Seger,
Human T-cell mitogen-activated protein kinase kinases are related to yeast signal transduction kinases.
1992,
Pubmed
Shibuya,
Mos induces the in vitro activation of mitogen-activated protein kinases in lysates of frog oocytes and mammalian somatic cells.
1993,
Pubmed
,
Xenbase
Shibuya,
Oncogenic ras triggers the activation of 42-kDa mitogen-activated protein kinase in extracts of quiescent Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Sturgill,
Insulin-stimulated MAP-2 kinase phosphorylates and activates ribosomal protein S6 kinase II.
1988,
Pubmed
,
Xenbase
Thomas,
MAP kinase by any other name smells just as sweet.
1992,
Pubmed
Van Aelst,
Complex formation between RAS and RAF and other protein kinases.
1993,
Pubmed
Vojtek,
Mammalian Ras interacts directly with the serine/threonine kinase Raf.
1993,
Pubmed
Watanabe,
Independent inactivation of MPF and cytostatic factor (Mos) upon fertilization of Xenopus eggs.
1991,
Pubmed
,
Xenbase
White,
The acidic transcriptional activator GAL-VP16 acts on preformed template-committed complexes.
1992,
Pubmed
Yan,
Identification of 2 serine residues of MEK-1 that are differentially phosphorylated during activation by raf and MEK kinase.
1994,
Pubmed
Yew,
Meiotic initiation by the mos protein in Xenopus.
1992,
Pubmed
,
Xenbase
Yocum,
Use of lacZ fusions to delimit regulatory elements of the inducible divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae.
1984,
Pubmed
Zheng,
Activation of MEK family kinases requires phosphorylation of two conserved Ser/Thr residues.
1994,
Pubmed
