Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1991 Nov 01;1011:3311-9. doi: 10.1002/j.1460-2075.1991.tb04895.x.
Show Gene links
Show Anatomy links
cdc2 phosphorylation is required for its interaction with cyclin.
Ducommun B
,
Brambilla P
,
Félix MA
,
Franza BR
,
Karsenti E
,
Draetta G
.
???displayArticle.abstract???
Activation of the cdc2 protein kinase at different stages of the cell cycle is regulated by post-translational modifications and interactions with cyclins. We show that in vitro translated human cdc2 binds very poorly to A and B cyclins, unless it has been preincubated with a Xenopus egg extract. This results in the phosphorylation of cdc2 which allows binding to cyclins. The replacement of Thr161, a residue conserved and phosphorylated in other protein kinases, with valine inhibits cdc2 association with A and B cyclins. In addition, mutations in the amino-terminus of cdc2 and within the conserved 'PSTAIR' region strongly inhibit binding. The Thr161Val mutation causes a lethal phenotype in the fission yeast Schizosaccharomyces pombe, while replacement of Thr161 with glutamic acid, potentially mimicking phosphorylation, causes uncoordination of mitosis and multiple cytokinesis. These results suggest that a threonine phosphorylation/dephosphorylation cycle is involved in regulating cdc2 function.
Ashkenazi,
Mapping the CD4 binding site for human immunodeficiency virus by alanine-scanning mutagenesis.
1990, Pubmed
Ashkenazi,
Mapping the CD4 binding site for human immunodeficiency virus by alanine-scanning mutagenesis.
1990,
Pubmed
Brizuela,
Activation of human CDC2 protein as a histone H1 kinase is associated with complex formation with the p62 subunit.
1989,
Pubmed
Broek,
Involvement of p34cdc2 in establishing the dependency of S phase on mitosis.
1991,
Pubmed
Carr,
Molecular cloning and sequence analysis of mutant alleles of the fission yeast cdc2 protein kinase gene: implications for cdc2+ protein structure and function.
1989,
Pubmed
Cunningham,
High-resolution epitope mapping of hGH-receptor interactions by alanine-scanning mutagenesis.
1989,
Pubmed
Draetta,
Human cdc2 protein kinase is a major cell-cycle regulated tyrosine kinase substrate.
,
Pubmed
Draetta,
Activation of cdc2 protein kinase during mitosis in human cells: cell cycle-dependent phosphorylation and subunit rearrangement.
1988,
Pubmed
Draetta,
Cdc2 protein kinase is complexed with both cyclin A and B: evidence for proteolytic inactivation of MPF.
1989,
Pubmed
Ducommun,
Cell cycle regulation of p34cdc2 kinase activity in Physarum polycephalum.
1990,
Pubmed
Dunphy,
Fission yeast p13 blocks mitotic activation and tyrosine dephosphorylation of the Xenopus cdc2 protein kinase.
1989,
Pubmed
,
Xenbase
Fantes,
Control of timing of cell cycle events in fission yeast by the wee 1+ gene.
1983,
Pubmed
Felix,
A post-ribosomal supernatant from activated Xenopus eggs that displays post-translationally regulated oscillation of its cdc2+ mitotic kinase activity.
1989,
Pubmed
,
Xenbase
Félix,
Triggering of cyclin degradation in interphase extracts of amphibian eggs by cdc2 kinase.
1990,
Pubmed
,
Xenbase
Félix,
Cdc2 H1 kinase is negatively regulated by a type 2A phosphatase in the Xenopus early embryonic cell cycle: evidence from the effects of okadaic acid.
1990,
Pubmed
,
Xenbase
Fleig,
Expression of a dominant negative allele of cdc2 prevents activation of the endogenous p34cdc2 kinase.
1991,
Pubmed
Franza,
Characterization of cellular proteins recognizing the HIV enhancer using a microscale DNA-affinity precipitation assay.
,
Pubmed
Garrels,
Quantitative two-dimensional gel electrophoresis of proteins.
1983,
Pubmed
Gautier,
Dephosphorylation and activation of Xenopus p34cdc2 protein kinase during the cell cycle.
1989,
Pubmed
,
Xenbase
Gautier,
Cyclin is a component of maturation-promoting factor from Xenopus.
1990,
Pubmed
,
Xenbase
Gibbs,
Rational scanning mutagenesis of a protein kinase identifies functional regions involved in catalysis and substrate interactions.
1991,
Pubmed
Giordano,
A 60 kd cdc2-associated polypeptide complexes with the E1A proteins in adenovirus-infected cells.
1989,
Pubmed
Gould,
Tyrosine phosphorylation of the fission yeast cdc2+ protein kinase regulates entry into mitosis.
1989,
Pubmed
Gould,
Complementation of the mitotic activator, p80cdc25, by a human protein-tyrosine phosphatase.
1990,
Pubmed
Hanks,
The protein kinase family: conserved features and deduced phylogeny of the catalytic domains.
1988,
Pubmed
Herskowitz,
Functional inactivation of genes by dominant negative mutations.
,
Pubmed
Hunt,
Maturation promoting factor, cyclin and the control of M-phase.
1989,
Pubmed
Jessus,
Direct activation of cdc2 with phosphatase: identification of p13suc1-sensitive and insensitive steps.
1990,
Pubmed
,
Xenbase
Krek,
Differential phosphorylation of vertebrate p34cdc2 kinase at the G1/S and G2/M transitions of the cell cycle: identification of major phosphorylation sites.
1991,
Pubmed
Kunkel,
Rapid and efficient site-specific mutagenesis without phenotypic selection.
1987,
Pubmed
Kuret,
Mutagenesis of the regulatory subunit of yeast cAMP-dependent protein kinase. Isolation of site-directed mutants with altered binding affinity for catalytic subunit.
1988,
Pubmed
Labbe,
Purification of MPF from starfish: identification as the H1 histone kinase p34cdc2 and a possible mechanism for its periodic activation.
1989,
Pubmed
,
Xenbase
Labbé,
MPF from starfish oocytes at first meiotic metaphase is a heterodimer containing one molecule of cdc2 and one molecule of cyclin B.
1989,
Pubmed
Lee,
INH, a negative regulator of MPF, is a form of protein phosphatase 2A.
1991,
Pubmed
,
Xenbase
Levin,
Association of catalytic and regulatory subunits of cyclic AMP-dependent protein kinase requires a negatively charged side group at a conserved threonine.
1990,
Pubmed
Matsushime,
Colony-stimulating factor 1 regulates novel cyclins during the G1 phase of the cell cycle.
1991,
Pubmed
Maundrell,
nmt1 of fission yeast. A highly transcribed gene completely repressed by thiamine.
1990,
Pubmed
Minshull,
The A- and B-type cyclin associated cdc2 kinases in Xenopus turn on and off at different times in the cell cycle.
1990,
Pubmed
,
Xenbase
Moreno,
Molecular genetic analysis of fission yeast Schizosaccharomyces pombe.
1991,
Pubmed
Morla,
Reversible tyrosine phosphorylation of cdc2: dephosphorylation accompanies activation during entry into mitosis.
1989,
Pubmed
Motokura,
A novel cyclin encoded by a bcl1-linked candidate oncogene.
1991,
Pubmed
Nurse,
Genetic control of cell size at cell division in yeast.
1975,
Pubmed
Pines,
Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from cyclin B.
1990,
Pubmed
Pines,
Isolation of a human cyclin cDNA: evidence for cyclin mRNA and protein regulation in the cell cycle and for interaction with p34cdc2.
1989,
Pubmed
Pondaven,
Activation of M-phase-specific histone H1 kinase by modification of the phosphorylation of its p34cdc2 and cyclin components.
1990,
Pubmed
Richardson,
An essential G1 function for cyclin-like proteins in yeast.
1989,
Pubmed
Simanis,
The cell cycle control gene cdc2+ of fission yeast encodes a protein kinase potentially regulated by phosphorylation.
1986,
Pubmed
Solomon,
Cyclin activation of p34cdc2.
1990,
Pubmed
,
Xenbase
Xiong,
Human D-type cyclin.
1991,
Pubmed
Zoller,
Oligonucleotide-directed mutagenesis: a simple method using two oligonucleotide primers and a single-stranded DNA template.
1987,
Pubmed
Zoller,
Affinity labeling of cAMP-dependent protein kinase with p-fluorosulfonylbenzoyl adenosine. Covalent modification of lysine 71.
1981,
Pubmed
