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Functional analysis of the sea urchin U7 small nuclear RNA.
Gilmartin GM
,
Schaufele F
,
Schaffner G
,
Birnstiel ML
.
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U7 small nuclear RNA (snRNA) is an essential component of the RNA-processing machinery which generates the 3' end of mature histone mRNA in the sea urchin. The U7 small nuclear ribonucleoprotein particle (snRNP) is classified as a member of the Sm-type U snRNP family by virtue of its recognition by both anti-trimethylguanosine and anti-Sm antibodies. We analyzed the function-structure relationship of the U7 snRNP by mutagenesis experiments. These suggested that the U7 snRNP of the sea urchin is composed of three important domains. The first domain encompasses the 5'-terminal sequences, up to about nucleotides 7, which are accessible to micrococcal nuclease, while the remainder of the RNA is highly protected and hence presumably bound by proteins. This region contains the sequence complementarities between the U7 snRNA and the histone pre-mRNA which have previously been shown to be required for 3' processing (F. Schaufele, G. M. Gilmartin, W. Bannwarth, and M. L. Birnstiel, Nature [London] 323:777-781, 1986). Nucleotides 9 to 20 constitute a second domain which includes sequences for Sm protein binding. The complementarities between the U7 snRNA sequences in this region and the terminal palindrome of the histone mRNA appear to be fortuitous and play only a secondary, if any, role in 3' processing. The third domain is composed of the terminal palindrome of U7 snRNA, the secondary structure of which must be maintained for the U7 snRNP to function, but its sequence can be drastically altered without any observable effect on snRNP assembly or 3' processing.
Ares,
U2 RNA from yeast is unexpectedly large and contains homology to vertebrate U4, U5, and U6 small nuclear RNAs.
1986, Pubmed
Ares,
U2 RNA from yeast is unexpectedly large and contains homology to vertebrate U4, U5, and U6 small nuclear RNAs.
1986,
Pubmed
Berget,
U1, U2, and U4/U6 small nuclear ribonucleoproteins are required for in vitro splicing but not polyadenylation.
1986,
Pubmed
Berk,
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
1977,
Pubmed
Black,
U2 as well as U1 small nuclear ribonucleoproteins are involved in premessenger RNA splicing.
1985,
Pubmed
Black,
Pre-mRNA splicing in vitro requires intact U4/U6 small nuclear ribonucleoprotein.
1986,
Pubmed
Branlant,
U2 RNA shares a structural domain with U1, U4, and U5 RNAs.
1982,
Pubmed
Brown,
Structure of the sea urchin U1 RNA repeat.
1985,
Pubmed
Busch,
SnRNAs, SnRNPs, and RNA processing.
1982,
Pubmed
Chabot,
The 3' splice site of pre-messenger RNA is recognized by a small nuclear ribonucleoprotein.
1985,
Pubmed
Ciliberto,
Formation of the 3' end on U snRNAs requires at least three sequence elements.
1986,
Pubmed
,
Xenbase
De Lorenzi,
Analysis of a sea urchin gene cluster coding for the small nuclear U7 RNA, a rare RNA species implicated in the 3' editing of histone precursor mRNAs.
1986,
Pubmed
De Robertis,
Intracellular transport of microinjected 5S and small nuclear RNAs.
1982,
Pubmed
,
Xenbase
Galli,
Biochemical complementation with RNA in the Xenopus oocyte: a small RNA is required for the generation of 3' histone mRNA termini.
1983,
Pubmed
,
Xenbase
Gick,
Generation of histone mRNA 3' ends by endonucleolytic cleavage of the pre-mRNA in a snRNP-dependent in vitro reaction.
1986,
Pubmed
Gold,
Reconstitution of RNAase P activity using inactive subunits from E. coli and HeLa cells.
1986,
Pubmed
Guerrier-Takada,
The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme.
1983,
Pubmed
Hashimoto,
A small nuclear ribonucleoprotein associates with the AAUAAA polyadenylation signal in vitro.
1986,
Pubmed
Hentschel,
Transcriptional fidelity of histone genes injected into Xenopus oocyte nuclei.
1980,
Pubmed
,
Xenbase
Hernandez,
Formation of the 3' end of U1 snRNA is directed by a conserved sequence located downstream of the coding region.
1985,
Pubmed
Krainer,
Multiple factors including the small nuclear ribonucleoproteins U1 and U2 are necessary for pre-mRNA splicing in vitro.
1985,
Pubmed
Krämer,
The 5' terminus of the RNA moiety of U1 small nuclear ribonucleoprotein particles is required for the splicing of messenger RNA precursors.
1984,
Pubmed
Kretzner,
S. cerevisiae U1 RNA is large and has limited primary sequence homology to metazoan U1 snRNA.
1987,
Pubmed
Lerner,
Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus.
1979,
Pubmed
Liautard,
Structural organization of ribonucleoproteins containing small nuclear RNAs from HeLa cells. Proteins interact closely with a similar structural domain of U1, U2, U4 and U5 small nuclear RNAs.
1982,
Pubmed
Mattaj,
Cap trimethylation of U snRNA is cytoplasmic and dependent on U snRNP protein binding.
1986,
Pubmed
,
Xenbase
Mattaj,
Nuclear segregation of U2 snRNA requires binding of specific snRNP proteins.
1985,
Pubmed
,
Xenbase
Moore,
Accurate cleavage and polyadenylation of exogenous RNA substrate.
1985,
Pubmed
Moore,
Site-specific polyadenylation in a cell-free reaction.
1984,
Pubmed
Mowry,
Both conserved signals on mammalian histone pre-mRNAs associate with small nuclear ribonucleoproteins during 3' end formation in vitro.
1987,
Pubmed
Patterson,
An essential yeast snRNA with a U5-like domain is required for splicing in vivo.
1987,
Pubmed
Probst,
Expression of sea urchin histone genes in the oocyte of Xenopus laevis.
1979,
Pubmed
,
Xenbase
Reddy,
Primary and secondary structure of U8 small nuclear RNA.
1985,
Pubmed
Riedel,
A subset of yeast snRNA's contains functional binding sites for the highly conserved Sm antigen.
1987,
Pubmed
,
Xenbase
Schaffner,
Genes and spacers of cloned sea urchin histone DNA analyzed by sequencing.
1978,
Pubmed
Schaufele,
Compensatory mutations suggest that base-pairing with a small nuclear RNA is required to form the 3' end of H3 messenger RNA.
,
Pubmed
,
Xenbase
Schaufele,
The inability of the Psammechinus miliaris H3 RNA to be processed in the Xenopus oocyte is associated with sequences distinct from those highly conserved amongst sea urchin histone RNAs.
1987,
Pubmed
,
Xenbase
Siliciano,
An essential snRNA from S. cerevisiae has properties predicted for U4, including interaction with a U6-like snRNA.
1987,
Pubmed
Sperry,
In vitro cleavage of the simian virus 40 early polyadenylation site adjacent to a required downstream TG sequence.
1986,
Pubmed
Strub,
The cDNA sequences of the sea urchin U7 small nuclear RNA suggest specific contacts between histone mRNA precursor and U7 RNA during RNA processing.
1984,
Pubmed
,
Xenbase
Strub,
Genetic complementation in the Xenopus oocyte: co-expression of sea urchin histone and U7 RNAs restores 3' processing of H3 pre-mRNA in the oocyte.
1986,
Pubmed
,
Xenbase
Weaver,
Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates.
1979,
Pubmed
Yuo,
Sequences required for 3' end formation of human U2 small nuclear RNA.
1985,
Pubmed
,
Xenbase
Zhuang,
A compensatory base change in U1 snRNA suppresses a 5' splice site mutation.
1986,
Pubmed
Zinn,
Identification of two distinct regulatory regions adjacent to the human beta-interferon gene.
1983,
Pubmed
