Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Molecular dissection of transjunctional voltage dependence in the connexin-32 and connexin-43 junctions.
Revilla A
,
Castro C
,
Barrio LC
.
???displayArticle.abstract???
Most gap junction channels are sensitive to the voltage difference between the two cellular interiors, termed the transjunctional voltage (V(j)). In several junctions, the conductance transitions induced by V(j) show more than one kinetic component. To elucidate the structural basis of the fast and slow components that characterize the V(j )dependence of connexin-32 (Cx32) and connexin-43 (Cx43) junctions, we created deletions of both connexins, where most of the carboxy-terminal (CT) domain was removed. The wild-type and "tailless" mutants were expressed in paired Xenopus oocytes, and the macroscopic gating properties were analyzed using the dual voltage clamp technique. Truncation of the CT domain of Cx32 and Cx43 abolished the fast mechanism of conductance transitions and induced novel gating properties largely attributable to the slow mechanism of gating. The formation of hybrid junctions comprising wild-type and truncated hemichannels allowed us to infer that the fast and slow components of gating reside in each hemichannel and that both gates close at a negative V(j) on the cytoplasmic side. Thus we conclude that the two kinetic components of V(j)-sensitive conductance are a result of the action of two different gating mechanisms. They constitute separate structures in the Cx32 and Cx43 molecules, the CT domain being an integral part of fast V(j) gating.
Armstrong,
Voltage-gated ion channels and electrical excitability.
1998, Pubmed
Armstrong,
Voltage-gated ion channels and electrical excitability.
1998,
Pubmed
Banach,
Connexin43 gap junctions exhibit asymmetrical gating properties.
1996,
Pubmed
Barrio,
Species-specific voltage-gating properties of connexin-45 junctions expressed in Xenopus oocytes.
1997,
Pubmed
,
Xenbase
Barrio,
Gap junctions formed by connexins 26 and 32 alone and in combination are differently affected by applied voltage.
1991,
Pubmed
,
Xenbase
Bennett,
Biophysics of gap junctions.
1992,
Pubmed
Bennett,
Gap junctions: new tools, new answers, new questions.
1991,
Pubmed
Beyer,
Connexin43: a protein from rat heart homologous to a gap junction protein from liver.
1987,
Pubmed
Bone,
New connexin32 mutations associated with X-linked Charcot-Marie-Tooth disease.
1995,
Pubmed
Bruzzone,
Connexin40, a component of gap junctions in vascular endothelium, is restricted in its ability to interact with other connexins.
1993,
Pubmed
,
Xenbase
Bruzzone,
Connections with connexins: the molecular basis of direct intercellular signaling.
1996,
Pubmed
Castro,
Altered formation of hemichannels and gap junction channels caused by C-terminal connexin-32 mutations.
1999,
Pubmed
,
Xenbase
Dunham,
Immunolocalization and expression of functional and nonfunctional cell-to-cell channels from wild-type and mutant rat heart connexin43 cDNA.
1992,
Pubmed
,
Xenbase
Ebihara,
Cloning and expression of a Xenopus embryonic gap junction protein.
1989,
Pubmed
,
Xenbase
Ek,
Role of histidine 95 on pH gating of the cardiac gap junction protein connexin43.
1994,
Pubmed
,
Xenbase
Fairweather,
Mutations in the connexin 32 gene in X-linked dominant Charcot-Marie-Tooth disease (CMTX1).
1994,
Pubmed
Goodenough,
Topological distribution of two connexin32 antigenic sites in intact and split rodent hepatocyte gap junctions.
1988,
Pubmed
Gupta,
Bovine connexin44, a lens gap junction protein: molecular cloning, immunologic characterization, and functional expression.
1994,
Pubmed
,
Xenbase
Harris,
Kinetic properties of a voltage-dependent junctional conductance.
1981,
Pubmed
Hennemann,
Molecular cloning and functional expression of mouse connexin40, a second gap junction gene preferentially expressed in lung.
1992,
Pubmed
,
Xenbase
Homma,
A particle-receptor model for the insulin-induced closure of connexin43 channels.
1998,
Pubmed
,
Xenbase
Hoshi,
Biophysical and molecular mechanisms of Shaker potassium channel inactivation.
1990,
Pubmed
,
Xenbase
Ionasescu,
Correlation between connexin 32 gene mutations and clinical phenotype in X-linked dominant Charcot-Marie-Tooth neuropathy.
1996,
Pubmed
Kumar,
Cloning and characterization of human and rat liver cDNAs coding for a gap junction protein.
1986,
Pubmed
Lal,
Voltage-dependent gating and single-channel conductance of adult mammalian atrial gap junctions.
1992,
Pubmed
Liu,
A structural basis for the unequal sensitivity of the major cardiac and liver gap junctions to intracellular acidification: the carboxyl tail length.
1993,
Pubmed
,
Xenbase
Makowski,
Gap junction structures. V. Structural chemistry inferred from X-ray diffraction measurements on sucrose accessibility and trypsin susceptibility.
1984,
Pubmed
Makowski,
Gap junction structures. II. Analysis of the x-ray diffraction data.
1977,
Pubmed
Martin,
Assembly of chimeric connexin-aequorin proteins into functional gap junction channels. Reporting intracellular and plasma membrane calcium environments.
1998,
Pubmed
,
Xenbase
Milks,
Topology of the 32-kd liver gap junction protein determined by site-directed antibody localizations.
1988,
Pubmed
Moreno,
Phosphorylation shifts unitary conductance and modifies voltage dependent kinetics of human connexin43 gap junction channels.
1992,
Pubmed
Moreno,
Gap junction channels: distinct voltage-sensitive and -insensitive conductance states.
1994,
Pubmed
Morley,
Intramolecular interactions mediate pH regulation of connexin43 channels.
1996,
Pubmed
,
Xenbase
Oh,
Changes in permeability caused by connexin 32 mutations underlie X-linked Charcot-Marie-Tooth disease.
1997,
Pubmed
,
Xenbase
Rabadan-Diehl,
A connexin-32 mutation associated with Charcot-Marie-Tooth disease does not affect channel formation in oocytes.
1994,
Pubmed
Sosinsky,
Image analysis of gap junction structures.
1992,
Pubmed
Sosinsky,
Molecular organization of gap junction membrane channels.
1996,
Pubmed
Spray,
Equilibrium properties of a voltage-dependent junctional conductance.
1981,
Pubmed
,
Xenbase
Suchyna,
Identification of a proline residue as a transduction element involved in voltage gating of gap junctions.
1993,
Pubmed
,
Xenbase
Unger,
Projection structure of a gap junction membrane channel at 7 A resolution.
1997,
Pubmed
Unwin,
Two configurations of a channel-forming membrane protein.
,
Pubmed
Unwin,
Structure of the junction between communicating cells.
1980,
Pubmed
Valiunas,
Conductances and selective permeability of connexin43 gap junction channels examined in neonatal rat heart cells.
1997,
Pubmed
Verselis,
Opposite voltage gating polarities of two closely related connexins.
1994,
Pubmed
,
Xenbase
Wang,
Gating of mammalian cardiac gap junction channels by transjunctional voltage.
1992,
Pubmed
White,
The connexin family of intercellular channel forming proteins.
1995,
Pubmed
Wilders,
Limitations of the dual voltage clamp method in assaying conductance and kinetics of gap junction channels.
1992,
Pubmed
Willecke,
Mouse connexin37: cloning and functional expression of a gap junction gene highly expressed in lung.
1991,
Pubmed
,
Xenbase
Yancey,
The 43-kD polypeptide of heart gap junctions: immunolocalization, topology, and functional domains.
1989,
Pubmed
Yeager,
Membrane topology and quaternary structure of cardiac gap junction ion channels.
1992,
Pubmed
Zimmer,
Topological analysis of the major protein in isolated intact rat liver gap junctions and gap junction-derived single membrane structures.
1987,
Pubmed
